FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8900880
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG95722710.754296204420237No Hit
CAACGGAATCCCAAAAGCAGCTG6152456.912181716863951No Hit
TAGCAGCACGTAAATATTGGCG4039414.538214199045488No Hit
TTCAAGTAATCCAGGATAGGCT2887573.2441399052677933No Hit
CCCCCACTGCTAAATTTGACTG2813713.1611593460421887No Hit
AACATTCAACGCTGTCGGTGAGT2575002.8929723802590304No Hit
AAGCTGCCAGTTGAAGAACTGT2400712.697160280781226No Hit
AACATTCAACGCTGTCGGTGAG1550411.7418614788650109No Hit
TAGCTTATCAGACTGATGTTGAC1421971.5975611400221104No Hit
TAGCTTATCAGACTGATGTTGA1308621.470214181069737No Hit
TAGCTTATCAGACTGATGTTGACT1288901.4480590683168406No Hit
TATTGCACTTGTCCCGGCCTGTAA1060941.1919495600435013No Hit
TATTGCACTTGTCCCGGCCTGTAT929081.0438069044858487No Hit
CATAAAGTAGAAAGCACTACT900701.0119224166599259No Hit
TGTAAACATCCTTGACTGGAAGCT771320.8665660024626778No Hit
TTCTCACTACTGCACTTGACTAG738820.8300527588283405No Hit
TTCAAGTAATTCAGGATAGGTT652730.7333319851520299No Hit
TATTGCACTTGTCCCGGCCTGT622210.6990432406683385No Hit
TGAGGTAGTAGGTTGTATAGTT614460.6903362364170734No Hit
CTTCTCACTACTGCACTTGACTAG548300.6160065072217579No Hit
TGAGGTAGTAGATTGTATAGTT478090.5371266661273941No Hit
TATTGCACTTGTCCCGGCCTGTA424710.4771550678135196No Hit
TGTAAACATCCCCGACTGGAAGCT419580.4713915927413919No Hit
AGCAGCATTGTACAGGGCTATGA394240.442922497550804No Hit
CAACGGAATCCCAAAAGCAGCTGT372230.41819460547721127No Hit
CTCCCACTGCTTCACTTGACTAG350150.39338806949425226No Hit
CCCCCCACTGCTAAATTTGACTGG337970.3797040292645222No Hit
CCCCCACTGCTAAATTTGACTGGCTT311860.35036985107090535No Hit
GGAATACCGGGTGCTGTAGGCTTT307980.34601073152317524No Hit
GGAATACCGGGTGCTGTAGGCTT255410.28694915558911027No Hit
CATTGCACTTGTCTCGGTCTGA249630.2804554156442958No Hit
CAAAGAATTCTCCTTTTGGGCT245250.2755345538868067No Hit
TGTAAACATCCTACACTCTCAGCT244200.27435489524631274No Hit
CCCATAAAGTAGAAAGCACT243740.2738380924133344No Hit
CCCATAAAGTAGAAAGCACTAC231020.25954737059706456No Hit
TAGCAGCACGTAAATATTGGC219990.24715533744978027No Hit
CCCCCCACTGCTAAATTTGACTGGCT219400.2464924816422646No Hit
ACTGGACTTGGAGTCAGAAGGC214020.24044813546525737No Hit
TGATGTAGTAGGTTGTATAGTT203550.22868525359290318No Hit
CAACGGAATCCCAAAAGCAGCT203450.22857290515095136No Hit
TAGCTTATCAGACTGATGTTGACA200130.22484293687815138No Hit
TGAGGTAGTAGTTTGTACAGTT198900.22346105104214414No Hit
ACCCCCCACTGCTAAATTTGACTG194090.21805709098426224No Hit
TGTCAGTTTGTCAAATACCCCA183120.20573246690214897No Hit
AACATTCAACGCTGTCGGTGAGTT174970.19657606888307672No Hit
TGAATACCGGGTGCTGTAGGCTTT174760.19634013715497792No Hit
CACCCGGCTGTGTGCACATGTGC172940.19429539551145505No Hit
CAAAGAATTCTCCTTTTGGGCTT166780.18737473148722372No Hit
TGTAAACATCCCCGACTGGAAGC164770.1851165278039924No Hit
TGATGTAGTAGATTGTATAGTT161470.1814090292195828No Hit
TGTCAGTTTGTCAAATACCCCAA157680.1771510232696093No Hit
TTCACAGTGGCTAAGTTCCG150070.1686013068370768No Hit
AGCTCGGTCTGAGGCCCCTCAGT148120.16641051221901654No Hit
CTCCCACTGCTTCACTTGACTAGCCT143920.16169187765704066No Hit
GACCCGCGGGCGCTCTCCAGTCCT143720.16146718077313704No Hit
TGAATACCGGGTGCTGTAGGCTT142140.15969207539029848No Hit
TTCAAGTAATCCAGGATAGGCTT134070.15062555612478767No Hit
TTCAAGTAATCCAGGATAGGC131210.14741239068496598No Hit
CAACACCAGTCGATGGGCTGT129420.14540135357402864No Hit
TGAGGTAGTAGTTTGTGCTGTT124990.1404243175955636No Hit
TATTGCACTTGTCCCGGCCTGTT121120.13607643289202867No Hit
TATTGCACTTGTCCCGGCCTGTAG120590.13548098614968407No Hit
AACGGAATCCCAAAAGCAGCTG119510.13426762297660458No Hit
CACTAGATTGTGAGCTCCTGGA118000.13257116150313228No Hit
ACCGGGTGCTGTAGGCTT116690.1310993969135636No Hit
CACTAGATTGTGAGCTCCTGGAA111440.12520110371109372No Hit
CCCCCACTGCTAAATTTGACTGG109370.12287549096269133No Hit
ACCGGGTGCTGTAGGCTTA104630.11755017481417566No Hit
AACCGGGCGGAAACACCA102850.11555037254743351No Hit
TTCACAGTGGCTAAGTTCTGC101820.11439318359532989No Hit
TTATAAAGCAATGAGACTGATT97150.10914651135618052No Hit
TGTCAGTTTGTCAAATACCCC95550.1073489362849516No Hit
CGCGACCTCAGATCAGACGTGGCG94000.10560753543469859No Hit
CAACGGAATCCCAAAAGCAGCTGA92800.1042593541312769No Hit
CTACGCCTGTCTGAGCGTCGCT92030.10339427112824799No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC91460.10275388500912269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)