FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8076779
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6507618.057184677208577No Hit
CAACGGAATCCCAAAAGCAGCTG6137257.598635545184535No Hit
TAGCAGCACGTAAATATTGGCG3665574.538405718418196No Hit
AAGCTGCCAGTTGAAGAACTGT3004643.720096835632125No Hit
TTCAAGTAATCCAGGATAGGCT2417592.9932600607247024No Hit
AACATTCAACGCTGTCGGTGAGT2062052.553059827438636No Hit
TAGCTTATCAGACTGATGTTGAC1970652.4398959040478885No Hit
CCCCCACTGCTAAATTTGACTG1781522.205730774607056No Hit
TAGCTTATCAGACTGATGTTGACT1361201.685325301088466No Hit
TGTAAACATCCTTGACTGGAAGCT1236341.5307339720450441No Hit
GCCTGTCTGAGCGTCGCT1222081.5130784190083695No Hit
AACATTCAACGCTGTCGGTGAG1033771.2799285457730118No Hit
TAGCTTATCAGACTGATGTTGA934081.1565006297683766No Hit
CATAAAGTAGAAAGCACTACT833411.0318593587864668No Hit
TATTGCACTTGTCCCGGCCTGTAA820781.0162219369875045No Hit
TATTGCACTTGTCCCGGCCTGTAT759950.9409072601837937No Hit
AGCAGCATTGTACAGGGCTATGA563190.6972952955627485No Hit
TTCAAGTAATTCAGGATAGGTT555410.6876627427839737No Hit
TCCTGTCTGAGCGTCGCT531390.6579231646674992No Hit
TGAGGTAGTAGGTTGTATAGTT516110.6390047319605996No Hit
TGTAAACATCCTACACTCTCAGCT492670.6099832618919002No Hit
TGTAAACATCCCCGACTGGAAGCT477070.5906686316414006No Hit
TTCTCACTACTGCACTTGACTAG475330.5885143074980757No Hit
TGTCAGTTTGTCAAATACCCCAA469600.5814198952329883No Hit
CAACGGAATCCCAAAAGCAGCTGT469190.5809122671302508No Hit
TATTGCACTTGTCCCGGCCTGT448140.5548498974653139No Hit
CCCCCACTGCTAAATTTGACTGGCTT435460.5391505698001642No Hit
CTTCTCACTACTGCACTTGACTAG432700.5357333659866143No Hit
TGAGGTAGTAGATTGTATAGTT417270.516629215681152No Hit
CCCATAAAGTAGAAAGCACTAC406740.5035918402620649No Hit
TGTCAGTTTGTCAAATACCCCA361530.4476165560553285No Hit
CCCCCCACTGCTAAATTTGACTGGCT348270.4311991203424038No Hit
GGAATACCGGGTGCTGTAGGCTTT313710.3884097856336047No Hit
CAAAGAATTCTCCTTTTGGGCT304780.37735339793251743No Hit
TATTGCACTTGTCCCGGCCTGTA298500.36957802113936755No Hit
ACTGGACTTGGAGTCAGAAGGC298170.3691694424225301No Hit
TAGCTTATCAGACTGATGTTGACA275580.3412003720790181No Hit
CAAAGAATTCTCCTTTTGGGCTT242840.30066441089944396No Hit
TGTAAACATCCTACACTCAGCT218250.27021910590843207No Hit
CATTGCACTTGTCTCGGTCTGA214020.2649818696289697No Hit
GGAATACCGGGTGCTGTAGGCTT205730.25471787701508236No Hit
TAGCAGCACGTAAATATTGGC202610.25085495096498245No Hit
AACCGGGCGGAAACACCA199860.24745012832467No Hit
TGAGGTAGTAGTTTGTACAGTT198650.24595200636293255No Hit
CCCCCCACTGCTAAATTTGACTGG196760.24361196462104512No Hit
AACATTCAACGCTGTCGGTGAGTT191110.23661660174185772No Hit
CTCCCACTGCTTCACTTGACTAG189610.2347594257562328No Hit
TGTAAACATCCCCGACTGGAAGC177690.2200010672571331No Hit
TGAATACCGGGTGCTGTAGGCTTT174720.21632385880559565No Hit
TGTCAGTTTGTCAAATACCCC172990.21418191583550816No Hit
CTCCCACTGCTTCACTTGACTAGCCT172060.21303046672442072No Hit
TGATGTAGTAGGTTGTATAGTT169990.21046756386425827No Hit
CACCCGGCTGTGTGCACATGTGC167860.2078303739646708No Hit
TAGCAGCACGTAAATATTGGCGT164300.2034226762921209No Hit
ATCACATTGCCAGGGATTTCCA162930.20172645555858343No Hit
CAACGGAATCCCAAAAGCAGCT152410.18870146131273371No Hit
ACCCCCCACTGCTAAATTTGACTG150110.18585379146810876No Hit
TTCAAGTAATCCAGGATAGGCTT139100.17222211973362153No Hit
CCCATAAAGTAGAAAGCACT137000.16962207335374657No Hit
TGATGTAGTAGATTGTATAGTT136330.16879253474683412No Hit
TTCACAGTGGCTAAGTTCCGC123240.15258557897894692No Hit
CGCCTGTCTGAGCGTCGCT120060.148648365889422No Hit
CTACGCCTGTCTGAGCGTCGCT116580.1443397176027721No Hit
TAGCAGCACATCATGGTTTACA116480.1442159058703971No Hit
TGAATACCGGGTGCTGTAGGCTT115250.1426930215621846No Hit
ATCACATTGCCAGGGATTTCC113880.14099680082864718No Hit
TGAGGTAGTAGTTTGTGCTGTT110800.13718339947149724No Hit
TTATAAAGCAATGAGACTGATT106250.13154996564843485No Hit
ACTTGACTTGGAGTCAGAAGGC105390.1304851847500099No Hit
TGTAAACATCCTTGACTGGAAGC102120.12643654110134744No Hit
TTCACAGTGGCTAAGTTCTGC101690.12590415065213495No Hit
TTCAAGTAATCCAGGATAGGC101430.12558224014796No Hit
AGCAGCATTGTACAGGGCTATGT98010.12134787890073506No Hit
TTCACAGTGGCTAAGTTCCG96610.1196145146474851No Hit
CCCCCACTGCTAAATTTGACTGGCT94160.11658112720429765No Hit
AACGGAATCCCAAAAGCAGCTG94100.11650684016487267No Hit
CAGTGCAATAGTATTGTCAAAGC92680.11474871356514768No Hit
CAAAGTGCTGTTCGTGCAGGTAG91730.11357250210758522No Hit
CACTAGATTGTGAGCTCCTGGAA91530.11332487864283522No Hit
TATTGCACTTGTCCCGGCCTGTAG90310.11181437550786026No Hit
TATTGCACTTGTCCCGGCCTGTT89420.11071245108972276No Hit
AGCTCGGTCTGAGGCCCCTCAGT88730.10985815013633529No Hit
TGTAGTGTTTCCTACTTTATGGA86250.10678761917343536No Hit
ATGCACCTGGGCAAGGATTCTGA84400.10449710212449789No Hit
ACTGGACTTGGAGTCAGAAGGCA83800.1037542317302479No Hit
CACTAGATTGTGAGCTCCTGGA81320.10068370076734796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)