FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12506698
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG152604612.20182977153522No Hit
CAACGGAATCCCAAAAGCAGCTG10312088.245245867454383No Hit
CCCCCACTGCTAAATTTGACTG6193804.952386313317872No Hit
AACATTCAACGCTGTCGGTGAGT3601542.8796889474743854No Hit
TAGCTTATCAGACTGATGTTGAC3474862.778399222560583No Hit
TAGCAGCACGTAAATATTGGCG3289542.6302226215104896No Hit
TTCAAGTAATCCAGGATAGGCT3034012.4259081014029444No Hit
TAGCTTATCAGACTGATGTTGACT2964102.3700100538127646No Hit
AACATTCAACGCTGTCGGTGAG2671082.1357195960116733No Hit
GCCTGTCTGAGCGTCGCT2383811.9060266746666468No Hit
AAGCTGCCAGTTGAAGAACTGT2297321.8368717306518474No Hit
TATTGCACTTGTCCCGGCCTGTAA2240451.7914000961724668No Hit
TGAGGTAGTAGGTTGTATAGTT1923871.5382717324748707No Hit
TATTGCACTTGTCCCGGCCTGTAT1847491.4772004569071708No Hit
TAGCTTATCAGACTGATGTTGA1837611.469300689918314No Hit
TGAGGTAGTAGATTGTATAGTT1315951.0521961911929112No Hit
TGTAAACATCCTTGACTGGAAGCT1156110.9243926734298693No Hit
AGCAGCATTGTACAGGGCTATGA974600.7792624400141428No Hit
TATTGCACTTGTCCCGGCCTGT876900.7011442988389102No Hit
TATTGCACTTGTCCCGGCCTGTA864450.6911896329470817No Hit
ACTGGACTTGGAGTCAGAAGGC863460.6903980571050807No Hit
CATAAAGTAGAAAGCACTACT799700.6394173745939975No Hit
TGTAAACATCCCCGACTGGAAGCT764460.6112404729049986No Hit
TTCAAGTAATTCAGGATAGGTT721520.5769068702226599No Hit
CATTGCACTTGTCTCGGTCTGA704300.563138248001191No Hit
CACCCGGCTGTGTGCACATGTGC664390.5312273471383094No Hit
CCCCCACTGCTAAATTTGACTGGCTT533390.42648347309577633No Hit
CAACGGAATCCCAAAAGCAGCTGT507250.40558267258072433No Hit
CAACGGAATCCCAAAAGCAGCT497960.3981546528108378No Hit
GGAATACCGGGTGCTGTAGGCTTT487710.38995904434567785No Hit
TAGCTTATCAGACTGATGTTGACA471310.37684607080142174No Hit
TGAGGTAGTAGTTTGTACAGTT449730.359591316588919No Hit
CCCATAAAGTAGAAAGCACTAC352220.2816250940096259No Hit
TTCTCACTACTGCACTTGACTAG335230.2680403732463997No Hit
CAACACCAGTCGATGGGCTGT330840.26453025410863845No Hit
CAAAGAATTCTCCTTTTGGGCT323970.25903719750808724No Hit
CACTAGATTGTGAGCTCCTGGA318020.2542797467405066No Hit
CTTCTCACTACTGCACTTGACTAG317990.2542557595937793No Hit
TCCTGTCTGAGCGTCGCT317830.254127828144567No Hit
TGTAAACATCCTACACTCTCAGCT317040.2534961666140815No Hit
GGAATACCGGGTGCTGTAGGCTT304210.24323766353037388No Hit
TGAGGTAGTAGTTTGTGCTGTT303740.2428618648983129No Hit
CCCATAAAGTAGAAAGCACT298500.23867210993661156No Hit
TTAATGCTAATCGTGATAGGGGTT289650.23159590165205876No Hit
CACTAGATTGTGAGCTCCTGGAA289560.23152394021187686No Hit
TGAGAACTGAATTCCATGGGTT284650.22759804386417581No Hit
TTATAAAGCAATGAGACTGATT267580.21394935737634344No Hit
TATTGCACTTGTCCCGGCCTGTAG267190.21363752446888856No Hit
CCCCCCACTGCTAAATTTGACTGGCT256900.20540993314142553No Hit
TAGCAGCACGTAAATATTGGC256400.2050101473626372No Hit
AACGGAATCCCAAAAGCAGCTG251320.20094832385014813No Hit
AACATTCAACGCTGTCGGTGAGTT250160.20002082084335931No Hit
CAAAGAATTCTCCTTTTGGGCTT249710.1996610136424498No Hit
TCAGTGCACTACAGAACTTTGT243550.19473565284777805No Hit
ACTGGACTTGGAGTCAGAAGGCT242240.19368821410735274No Hit
TATTGCACTTGTCCCGGCCTGTAAT240570.19235292960619982No Hit
TGAGAACTGAATTCCATAGGCTGT239130.19120154656328953No Hit
ACCCCCCACTGCTAAATTTGACTG237990.1902900349876522No Hit
ACTGGACTTGGAGTCAGAAGGCA233510.1867079544097091No Hit
CTCCCACTGCTTCACTTGACTAGCCT231410.18502885413879827No Hit
TCAGTGCACTACAGAACTTTGTC224380.17940786608903483No Hit
AGCAGCATTGTACAGGGCTATGT220030.17592972981357669No Hit
CTCCCACTGCTTCACTTGACTAG196920.15745163111798174No Hit
TTCAAGTAATCCAGGATAGGCTT193320.154573173510706No Hit
TGTCAGTTTGTCAAATACCCCA180270.14413876468433154No Hit
CCCCCCACTGCTAAATTTGACTGG177630.14202789577232935No Hit
TATTGCACTTGTCCCGGCCTGTT176650.14124431564590428No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC176170.14086052129826754No Hit
ATGCACCTGGGCAAGGATTCTGA175680.14046873123505502No Hit
ACCGGGTGCTGTAGGCTT172440.13787811938850686No Hit
TGTAAACATCCCCGACTGGAAGC168670.13486373461644313No Hit
TATTGCACTTGTCCCGGCCTGTAAA161890.12944263945607387No Hit
AATGCACCCGGGCAAGGATTCT161510.12913880226419477No Hit
ACTGGACTTGGAGTCAGAAGG159420.12746769770885968No Hit
TGTCAGTTTGTCAAATACCCCAA152500.12193466253042969No Hit
CAGTGCAATAGTATTGTCAAAGC152270.12175076107218707No Hit
TGAGGTAGTAAGTTGTATTGTT150810.12058338659812526No Hit
CAACGGAATCCCAAAAGCAGCTGA147610.11802475761388018No Hit
AGCAGCATTGTACAGGGCTATG143340.11461058706302815No Hit
TAGCTTATCAGACTGATGTTGACC139580.11160419800654017No Hit
CTACGGGGATGATTTTACGAACTGAA134820.10779823739247561No Hit
TCAGTGCATCACAGAACTTTGTTT133000.10634301715768622No Hit
TTCACAGTGGCTAAGTTCTGC130730.10452798972198737No Hit
ACCGGGTGCTGTAGGCTTT130580.10440805398835087No Hit
GCCCCCACTGCTAAATTTGACTG128720.10292085089125844No Hit
ACCACAGGGTAGAACCACGGAC127170.10168151497701472No Hit
AACCGGGCGGAAACACCA126510.10115379774901416No Hit
CAAAGTGCTGTTCGTGCAGGTAG125800.10058610194313479No Hit
CCCATAAAGTAGAAAGCACTA125670.10048215764064984No Hit
TTCACAGTGGCTAAGTTCCGC125500.10034623047586182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)