FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8532385
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8464999.9210127062949No Hit
CCCCCCACTGCTAAATTTGACTG7157378.388475203591963No Hit
TAGCAGCACGTAAATATTGGCG3296073.863011338564774No Hit
AACATTCAACGCTGTCGGTGAGT3072153.600575923378985No Hit
CCCCCACTGCTAAATTTGACTG2734793.205188232832907No Hit
AAGCTGCCAGTTGAAGAACTGT2727653.196820115360476No Hit
TTCAAGTAATCCAGGATAGGCT2608863.057597611922106No Hit
AACATTCAACGCTGTCGGTGAG2085962.444756067617671No Hit
TAGCTTATCAGACTGATGTTGAC1285611.5067416671891856No Hit
TAGCTTATCAGACTGATGTTGACT1191241.3961395319128238No Hit
TATTGCACTTGTCCCGGCCTGTAA1145531.3425671720157963No Hit
TAGCTTATCAGACTGATGTTGA1065291.2485254708970586No Hit
CATAAAGTAGAAAGCACTACT1062051.2447281738927627No Hit
TATTGCACTTGTCCCGGCCTGTAT1011581.185577069014115No Hit
TGAGGTAGTAGGTTGTATAGTT906301.0621883564794603No Hit
TATTGCACTTGTCCCGGCCTGT864321.012987576158366No Hit
TGTAAACATCCTTGACTGGAAGCT768280.9004281921174443No Hit
TGAGGTAGTAGATTGTATAGTT640030.7501185190307282No Hit
TTCAAGTAATTCAGGATAGGTT614310.7199745440460082No Hit
TATTGCACTTGTCCCGGCCTGTA486060.5696648709592921No Hit
CAACGGAATCCCAAAAGCAGCTGT446760.5236050647034797No Hit
TGTAAACATCCCCGACTGGAAGCT429120.502930892124535No Hit
AGCAGCATTGTACAGGGCTATGA428240.5018995275060841No Hit
CACCCGGCTGTGTGCACATGTGC404990.47465040548451576No Hit
CATTGCACTTGTCTCGGTCTGA380370.4457956362728593No Hit
TTCTCACTACTGCACTTGACTAG362610.42498082306412566No Hit
TGATGTAGTAGGTTGTATAGTT346790.40643970003697677No Hit
CCCATAAAGTAGAAAGCACTAC343800.4029354043447407No Hit
CCCATAAAGTAGAAAGCACT339340.3977082609375925No Hit
ACTGGACTTGGAGTCAGAAGGC314200.3682440489968514No Hit
TGAGAACTGAATTCCATGGGTT291660.3418270507015331No Hit
TGTCAGTTTGTCAAATACCCCAA291380.341498889232026No Hit
TGAGGTAGTAGTTTGTACAGTT271000.31761342227290495No Hit
CAACGGAATCCCAAAAGCAGCT253960.29764245284290386No Hit
TCGTACGACTCTTAGCGG250700.2938217157336431No Hit
CTTCTCACTACTGCACTTGACTAG248520.29126674429248095No Hit
TGATGTAGTAGATTGTATAGTT240410.2817617817292586No Hit
CAACACCAGTCGATGGGCTGT238010.27894896913348377No Hit
TGTAAACATCCTACACTCTCAGCT209020.24497253698702062No Hit
CCCCCACTGCTAAATTTGACTGGCTT197940.23198671883652694No Hit
AACATTCAACGCTGTCGGTGAGTT197070.2309670742705586No Hit
TGTCAGTTTGTCAAATACCCCA194730.22822458198967815No Hit
TAGCAGCACGTAAATATTGGC188100.22045418719385027No Hit
CAAAGAATTCTCCTTTTGGGCT166190.19477555220492276No Hit
CTCCCACTGCTTCACTTGACTAG162710.1906969739410493No Hit
AACGGAATCCCAAAAGCAGCTG161690.18950152858784503No Hit
GGAATACCGGGTGCTGTAGGCTTT158520.1857862719509258No Hit
TGAGGTAGTAGTTTGTGCTGTT155670.1824460569934432No Hit
CGCCTGTCTGAGCGTCGCT153360.17973872487000997No Hit
TGAGAACTGAATTCCATAGGCTGT152910.1792113225083022No Hit
TTAATGCTAATCGTGATAGGGGTT149000.17462878198768575No Hit
TGTAAACATCCCCGACTGGAAGC146440.17162844855219261No Hit
CAAAGAATTCTCCTTTTGGGCTT144170.16896799663868894No Hit
TATTGCACTTGTCCCGGCCTGTT140680.1648776983223331No Hit
TAGCTTATCAGACTGATGTTGACA138320.16211176593648788No Hit
ACCCCCCACTGCTAAATTTGACTG135900.1592755132357483No Hit
CTACGCCTGTCTGAGCGTCGCT135010.15823242856481512No Hit
ACTTGACTTGGAGTCAGAAGGC133600.15657990116479742No Hit
CCCCCCACTGCTAAATTTGACTGGCT131640.154282770878248No Hit
TTCACAGTGGCTAAGTTCCGC130500.15294668489525498No Hit
TTATAAAGCAATGAGACTGATT129440.1517043593321211No Hit
GGAATACCGGGTGCTGTAGGCTT128910.15108319655055416No Hit
TTCAAGTAATCCAGGATAGGCTT128600.15071987492359992No Hit
TATTGCACTTGTCCCGGCCTGTAG128500.15060267439877595No Hit
CCCCCCACTGCTAAATTTGACTGG128300.15036827334912806No Hit
CACTAGATTGTGAGCTCCTGGA124950.14644205576752573No Hit
ACCGGGTGCTGTAGGCTT122150.14316044107245512No Hit
TTCACAGTGGCTAAGTTCTGC121210.14205875613910998No Hit
GCCTGTCTGAGCGTCGCT118060.13836693960715557No Hit
TTCAAGTAATCCAGGATAGGC112340.13166306958722562No Hit
AACCGGGCGGAAACACCA110790.1298464614524544No Hit
TGATAACTGAATTCCATGGGTT104070.12197058618428494No Hit
AACATTCAACGCTGTCGGTGA101580.11905229311616858No Hit
TGCAAGTAATCCAGGATAGGCT101370.11880617201403829No Hit
TGATGTAGTAGTTTGTACAGTT100090.1173060052962917No Hit
CCCATAAAGTAGAAAGCACTA99220.11628636073032333No Hit
ACCGGGTGCTGTAGGCTTA97460.11422363149342182No Hit
TCGTTTCCCGGCCAATGCACCA96990.11367278902674927No Hit
CACTAGATTGTGAGCTCCTGGAA96230.11278206503808726No Hit
ATCCCGGACGAGCCCCCA96120.1126531444607809No Hit
ACCGGGTGCTGTAGGCTTT94570.11083653632600968No Hit
AGCTCGGTCTGAGGCCCCTCAGT93820.10995753238983003No Hit
CTCCCACTGCTTCACTTGACTAGCCT91610.10736740079122076No Hit
AAGCTGCCAGTTGAAGCACTGT91290.1069923591117841No Hit
TATTGCACTTGTCCCGGCCTGTAAT89330.10469522882523467No Hit
ACTGGACTTGGAGTCAGAAGGCT88090.1032419423174177No Hit
TGTAAACATCCTACACTCAGCT87140.10212853733159016No Hit
TTCACAGTGGCTAAGTTCTG86060.1008627716634915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)