FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9815804
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8093308.245172784623653No Hit
CAACGGAATCCCAAAAGCAGCTG7228397.364032533656947No Hit
GCCTGTCTGAGCGTCGCT4764314.85371346045622No Hit
TTCAAGTAATCCAGGATAGGCT3422043.486255430528156No Hit
CCCCCACTGCTAAATTTGACTG3319183.381465237080936No Hit
TAGCAGCACGTAAATATTGGCG2472432.518825763024608No Hit
AAGCTGCCAGTTGAAGAACTGT2403152.4482457066176138No Hit
AACATTCAACGCTGTCGGTGAGT2257752.3001172395047824No Hit
AACATTCAACGCTGTCGGTGAG1873111.908259374372186No Hit
TGAGGTAGTAGGTTGTATAGTT1733981.766518565366627No Hit
TAGCTTATCAGACTGATGTTGAC1594731.6246555045312643No Hit
TAGCTTATCAGACTGATGTTGACT1435851.4627940818704204No Hit
CATAAAGTAGAAAGCACTACT1433241.4601351045721778No Hit
TATTGCACTTGTCCCGGCCTGTAT1402591.4289099497096722No Hit
TAGCTTATCAGACTGATGTTGA1398291.424529259141686No Hit
TGAGGTAGTAGATTGTATAGTT1300361.3247615783689244No Hit
TATTGCACTTGTCCCGGCCTGTAA1238131.2613638169629304No Hit
TATTGCACTTGTCCCGGCCTGT1137221.1585602157500292No Hit
TTCAAGTAATTCAGGATAGGTT900040.9169294741419042No Hit
TGTAAACATCCTTGACTGGAAGCT804910.8200143360645751No Hit
TATTGCACTTGTCCCGGCCTGTA794120.809021859034675No Hit
TCCTGTCTGAGCGTCGCT734620.7484053267567282No Hit
CATTGCACTTGTCTCGGTCTGA546320.5565718304888728No Hit
AGCAGCATTGTACAGGGCTATGA543560.5537600384033747No Hit
TGAGGTAGTAGTTTGTACAGTT489690.49887915447374453No Hit
ACCGGGTGCTGTAGGCTTA435190.443356448437642No Hit
ACCGGGTGCTGTAGGCTT417300.4251307381443231No Hit
TCGTACGACTCTTAGCGG413060.42081117349123925No Hit
CCCATAAAGTAGAAAGCACT401380.408911995390291No Hit
ACTGGACTTGGAGTCAGAAGGC395280.402697527375241No Hit
TTAATGCTAATCGTGATAGGGGTT380900.38804768310369686No Hit
TGTAAACATCCCCGACTGGAAGCT373660.3806718227055063No Hit
CCCATAAAGTAGAAAGCACTAC366510.37338765117966904No Hit
CAACGGAATCCCAAAAGCAGCTGT359450.3661951685261849No Hit
CACCCGGCTGTGTGCACATGTGC351260.357851481142044No Hit
CAACACCAGTCGATGGGCTGT323530.3296011208047756No Hit
ACCGGGTGCTGTAGGCTTT320570.32658557566960383No Hit
GGAATACCGGGTGCTGTAGGCTTT288050.29345532979264866No Hit
ATCCCGGACGAGCCCCCA284600.28994058968577613No Hit
TTCTCACTACTGCACTTGACTAG271630.2767272044144321No Hit
TGTAAACATCCTACACTCTCAGCT267350.2723668891514134No Hit
TGAGAACTGAATTCCATGGGTT250570.255272008283784No Hit
TTATAAAGCAATGAGACTGATT247180.25181839409181356No Hit
CTCCCACTGCTTCACTTGACTAG246840.2514720139073682No Hit
TGAGGTAGTAGTTTGTGCTGTT241970.24651062714781186No Hit
TCAGTGCACTACAGAACTTTGT230430.23475407618163527No Hit
TATTGCACTTGTCCCGGCCTGTT223670.22786722310266178No Hit
AACCGGGCGGAAACACCA214220.2182398915055761No Hit
GGAATACCGGGTGCTGTAGGCTT212390.21637555110106108No Hit
CACTAGATTGTGAGCTCCTGGA209340.2132683170935361No Hit
AACGGAATCCCAAAAGCAGCTG209340.2132683170935361No Hit
CAACGGAATCCCAAAAGCAGCT199890.2036409854964504No Hit
CTTCTCACTACTGCACTTGACTAG173840.17710215077644176No Hit
TATTGCACTTGTCCCGGCCTGTAG171750.17497293140735082No Hit
CACTAGATTGTGAGCTCCTGGAA171220.17443298582571534No Hit
CGCCTGTCTGAGCGTCGCT168530.17169250730760313No Hit
TTCACAGTGGCTAAGTTCCG164220.16730162908713336No Hit
TTCAAGTAATCCAGGATAGGC162270.1653150368528141No Hit
CAAAGAATTCTCCTTTTGGGCT160690.1637053877603913No Hit
AACATTCAACGCTGTCGGTGAGTT160600.16361369888803812No Hit
AGCTCGGTCTGAGGCCCCTCAGT160270.16327750635607638No Hit
TAGCTTATCAGACTGATGTTGACA157630.16058796610038262No Hit
TTCAAGTAATCCAGGATAGGCTT154900.15780673697233563No Hit
TGAGGTAGTAGGTTGTATAGT154260.15715472721337956No Hit
TTCACAGTGGCTAAGTTCTG153390.15626840144729864No Hit
TAGCAGCACGTAAATATTGGC146280.1490249805313961No Hit
TGTCAGTTTGTCAAATACCCCA144730.14744589439642436No Hit
CCCCCACTGCTAAATTTGACTGGCTT140430.1430652038284383No Hit
TGTCAGTTTGTCAAATACC129490.13191991201128303No Hit
AGCAGCATTGTACAGGGCTATGT128070.13047326535859924No Hit
ACCCCCCACTGCTAAATTTGACTG127940.1303408258763113No Hit
CCCATAAAGTAGAAAGCACTA126150.12851723608173105No Hit
TATTGCACTTGTCCCGGCCTGTTT125310.1276614732731012No Hit
TGAGGTAGTAGATTGTATAGT123550.12586844643597203No Hit
TGATGTAGTAGGTTGTATAGTT123020.12532850085433653No Hit
TGAGGTAGTAAGTTGTATTGTT122890.12519606137204858No Hit
TTCACAGTGGCTAAGTTCTGC113600.11573173221470193No Hit
TGAGGTAGTAGGTTGTGTGGTT112060.11416283373221389No Hit
TATTGCACTTGTCCCGGCCTGA111940.11404058190240964No Hit
TGTCAGTTTGTCAAATACCCCAA111750.11384701650521954No Hit
TTCACAGTGGCTAAGTTCCGC109710.11176873539854708No Hit
CCCCCCACTGCTAAATTTGACTGGCT107300.10931351114997813No Hit
AGAGGTAGTAGGTTGCATAGTT107060.10906900749036962No Hit
AGCTACATTGTCTGCTGGGTTT103840.1057885833906219No Hit
CCCCCCACTGCTAAATTTGACTGG102360.10428081082303599No Hit
TGTCAGTTTGTCAAATACCCC102320.10424006021310123No Hit
AGCAGCATTGTACAGGGCTATG101650.10355748749669412No Hit
ACTGGACTTGGAGTCAGAAGG101580.10348617392930828No Hit
TGTAAACATCCTACACTCAGCT100260.1021414038014614No Hit
TCAGTGCACTACAGAACTTTGTC99660.10153014465244009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)