FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7139852
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5315017.444145901063496No Hit
CCCCCCACTGCTAAATTTGACTG4925306.898322262142129No Hit
AACATTCAACGCTGTCGGTGAGT2485273.480842460039788No Hit
GGAATACCGGGTGCTGTAGGCTTT2284463.1995901315601496No Hit
TAGCAGCACGTAAATATTGGCG2075472.906880982967154No Hit
CCCCCACTGCTAAATTTGACTG1847542.5876446738671897No Hit
GGAATACCGGGTGCTGTAGGCTT1772322.482292350037508No Hit
AACATTCAACGCTGTCGGTGAG1618852.267343916932732No Hit
TTCAAGTAATCCAGGATAGGCT1614752.261601500983494No Hit
AAGCTGCCAGTTGAAGAACTGT1508172.11232669808842No Hit
TGAATACCGGGTGCTGTAGGCTTT1008921.4130825120744799No Hit
TGAATACCGGGTGCTGTAGGCTT788251.1040144809724346No Hit
TGAGGTAGTAGGTTGTATAGTT785631.100344937121946No Hit
TAGCTTATCAGACTGATGTTGAC768921.0769410906556607No Hit
TATTGCACTTGTCCCGGCCTGTAA747261.0466043273726122No Hit
TAGCTTATCAGACTGATGTTGA675240.9457338891618481No Hit
TAGCTTATCAGACTGATGTTGACT674400.9445573941868823No Hit
TATTGCACTTGTCCCGGCCTGTAT613840.8597377088488669No Hit
TATTGCACTTGTCCCGGCCTGT574460.8045825039510623No Hit
TGAGGTAGTAGATTGTATAGTT522370.7316258096106194No Hit
TTCAAGTAATTCAGGATAGGTT517960.7254492109920485No Hit
CATAAAGTAGAAAGCACTACT461870.6468901596279586No Hit
TGTAAACATCCTTGACTGGAAGCT427280.5984437772659713No Hit
AGCAGCATTGTACAGGGCTATGA372890.5222657276369314No Hit
GGAATACCGGGTGCTGTAGGCTTA361330.5060749158385917No Hit
TATTGCACTTGTCCCGGCCTGTA344280.4821948690252963No Hit
TTCTCACTACTGCACTTGACTAG320410.44876280348668296No Hit
TGTAAACATCCCCGACTGGAAGCT295330.4136360249484163No Hit
GGAATACCGGGTGCTGTAGGCT281110.39371964572935125No Hit
CGCCTGTCTGAGCGTCGCT279400.39132463810174223No Hit
ACTGGACTTGGAGTCAGAAGGC278450.389994078308626No Hit
CACCCGGCTGTGTGCACATGTGC239860.33594533892299167No Hit
CATTGCACTTGTCTCGGTCTGA233190.32660340858606035No Hit
TCGTACGACTCTTAGCGG232640.32583308449530884No Hit
CAACGGAATCCCAAAAGCAGCTGT232170.32517480754503036No Hit
CTTCTCACTACTGCACTTGACTAG231680.3244885188096336No Hit
TGAGGTAGTAGTTTGTACAGTT216830.30368976835934414No Hit
CCCATAAAGTAGAAAGCACT201220.28182657007456174No Hit
TGATGTAGTAGGTTGTATAGTT199140.27891334442226534No Hit
CTCCCACTGCTTCACTTGACTAG177520.24863260470945336No Hit
CAACACCAGTCGATGGGCTGT171080.2396128099013817No Hit
CTACGCCTGTCTGAGCGTCGCT170290.23850634438921142No Hit
CCCATAAAGTAGAAAGCACTAC166910.23377235270422972No Hit
AACATTCAACGCTGTCGGTGAGTT165550.23186755131618975No Hit
TGAATACCGGGTGCTGTAGGCTTA165420.23168547471292122No Hit
CCCCCACTGCTAAATTTGACTGGCTT152890.214136091336347No Hit
ACCGGGTGCTGTAGGCTT145140.20328152460303098No Hit
TAGCAGCACGTAAATATTGGC140310.19651667849697724No Hit
CCCCCCACTGCTAAATTTGACTGG138310.19371549998515375No Hit
TGAGGTAGTAGTTTGTGCTGTT133610.18713273048236856No Hit
TGATGTAGTAGATTGTATAGTT131390.18402342233424446No Hit
ATCCCGGACGAGCCCCCA128610.1801297842028098No Hit
TGAATACCGGGTGCTGTAGGCT127640.17877121262457543No Hit
AACGGAATCCCAAAAGCAGCTG123410.17284672007206872No Hit
ACCGGGTGCTGTAGGCTTT123100.17241253740273607No Hit
ACCGGGTGCTGTAGGCTTA120260.1684348639159467No Hit
TGTCAGTTTGTCAAATACCCCAA118440.16588579147018734No Hit
ATACCGGGTGCTGTAGGCTTA115900.1623282947601715No Hit
CCCCCCACTGCTAAATTTGACTGGCT114800.16078764657866856No Hit
AACCGGGCGGAAACACCA114580.160479516942368No Hit
CAACGGAATCCCAAAAGCAGCT112790.15797246217428595No Hit
GAATACCGGGTGCTGTAGGCTTT110810.1551992954475807No Hit
GCCTGTCTGAGCGTCGCT106760.14952690896113813No Hit
ATACCGGGTGCTGTAGGCTTT102730.1438825342598138No Hit
TGTAAACATCCTACACTCTCAGCT101490.14214580358248322No Hit
TGTAAACATCCCCGACTGGAAGC101200.1417396326982688No Hit
ATACCGGGTGCTGTAGGCTT99670.13959673113672383No Hit
CAAAGAATTCTCCTTTTGGGCT96940.13577312246808476No Hit
TGTCAGTTTGTCAAATACCCCA96440.13507282784012892No Hit
AGCTCGGTCTGAGGCCCCTCAGT96050.13452659803032332No Hit
GGGAATACCGGGTGCTGTAGGCTTT96030.13449858624520508No Hit
TAGCTTATCAGACTGATGTTGACA95570.1338543151874857No Hit
TTCAAGTAATCCAGGATAGGCTT95330.13351817376606687No Hit
TACGCCTGTCTGAGCGTCGCT94080.13176743719617717No Hit
TATTGCACTTGTCCCGGCCTGTT92660.1297786004527825No Hit
TTATAAAGCAATGAGACTGATT91400.12801385799033368No Hit
CAAAGAATTCTCCTTTTGGGCTT91360.12795783442009723No Hit
ACCCCCCACTGCTAAATTTGACTG90550.1268233571228087No Hit
AGAATTACTCTGAGACCT90400.12661326873442194No Hit
GGGAATACCGGGTGCTGTAGGCTT90250.12640318034603518No Hit
GAATACCGGGTGCTGTAGGCTT86580.12126301777683907No Hit
TATTGCACTTGTCCCGGCCTGTAG86050.12052070547120584No Hit
ACTGGACTTGGAGTCAGAAGG82190.1151144309433865No Hit
AATGACACGATCACTCCCGTTG79110.11080061603517832No Hit
TGAGGTAGTAGGTTGTGTGGTT78970.11060453353935068No Hit
ACTTGACTTGGAGTCAGAAGGC78270.10962412106021245No Hit
CTCCCACTGCTTCACTTGACTAGCCT77420.10843362019268747No Hit
AACATTCAACGCTGTCGGTGA76250.10679493076327072No Hit
TTCACAGTGGCTAAGTTCCG75700.10602460667251926No Hit
TTCAAGTAATCCAGGATAGGC75180.10529630025944517No Hit
ACGCCTGTCTGAGCGTCGCT75070.10514223544129486No Hit
CACTAGATTGTGAGCTCCTGGA74680.10459600563148927No Hit
AGCAGCATTGTACAGGGCTATG74130.1038256815407378No Hit
TTCACAGTGGCTAAGTTCTG73730.10326544583837312No Hit
GTAATACCGGGTGCTGTAGGCTTT72660.10176681533454754No Hit
CAAAGTGCTGTTCGTGCAGGT72240.10117856784706462No Hit
TGAGGTAGTAGGTTGTATAGT71970.10080040874796843No Hit
ACTGGACTTGGAGTCAGAAGGCT71720.10045026143399051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)