FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10780799
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7516956.972535152542961No Hit
CCCCCCACTGCTAAATTTGACTG6111415.668791339120598No Hit
TAGCAGCACGTAAATATTGGCG3937763.6525678662592633No Hit
GCCTGTCTGAGCGTCGCT3789603.5151383492076977No Hit
TTCAAGTAATCCAGGATAGGCT3786493.5122535908516612No Hit
AAGCTGCCAGTTGAAGAACTGT3774223.500872245183312No Hit
TAGCTTATCAGACTGATGTTGAC2594372.4064728412059253No Hit
AACATTCAACGCTGTCGGTGAGT2507242.3256532284851987No Hit
CCCCCACTGCTAAATTTGACTG2268772.104454410104483No Hit
TAGCTTATCAGACTGATGTTGACT1559161.4462378901600892No Hit
TAGCTTATCAGACTGATGTTGA1365511.2666129848075267No Hit
AACATTCAACGCTGTCGGTGAG1356131.2579123309877125No Hit
TCCTGTCTGAGCGTCGCT1289451.1960616277142353No Hit
TGTAAACATCCTTGACTGGAAGCT1014440.940969217587676No Hit
CATAAAGTAGAAAGCACTACT952130.8831720172131954No Hit
TGAGGTAGTAGGTTGTATAGTT900210.8350123214429653No Hit
TATTGCACTTGTCCCGGCCTGTAA878470.8148468402017328No Hit
TATTGCACTTGTCCCGGCCTGTAT792260.7348805965123735No Hit
TATTGCACTTGTCCCGGCCTGT755950.7012003470243717No Hit
AGCAGCATTGTACAGGGCTATGA734520.6813224140437086No Hit
CCCATAAAGTAGAAAGCACTAC619170.5743266338608113No Hit
TTCAAGTAATTCAGGATAGGTT584770.5424180526879316No Hit
CAACGGAATCCCAAAAGCAGCTGT537270.49835823856840294No Hit
TGAGGTAGTAGATTGTATAGTT517040.4795933956286543No Hit
TGTAAACATCCCCGACTGGAAGCT481640.4467572394216792No Hit
GGAATACCGGGTGCTGTAGGCTTT478240.4436034843057551No Hit
TGTCAGTTTGTCAAATACCCCAA461670.4282335659907953No Hit
TATTGCACTTGTCCCGGCCTGTA416720.3865390682082098No Hit
TGTAAACATCCTACACTCTCAGCT414780.3847395726420648No Hit
CGCCTGTCTGAGCGTCGCT396830.3680896007800535No Hit
GGAATACCGGGTGCTGTAGGCTT395100.36648489597106854No Hit
TTCTCACTACTGCACTTGACTAG372230.34527125494130817No Hit
CCCCCACTGCTAAATTTGACTGGCTT359810.333750773017844No Hit
CATTGCACTTGTCTCGGTCTGA359320.3332962612511373No Hit
ACTGGACTTGGAGTCAGAAGGC335500.31120142393898637No Hit
TGTCAGTTTGTCAAATACCCCA330920.3069531302828297No Hit
TAGCTTATCAGACTGATGTTGACA328390.3046063654465685No Hit
CTTCTCACTACTGCACTTGACTAG326150.30252859737019494No Hit
CTCCCACTGCTTCACTTGACTAG296300.27484048260244903No Hit
CTACGCCTGTCTGAGCGTCGCT293460.27220616950561827No Hit
CCCCCCACTGCTAAATTTGACTGGCT285270.2646093299763774No Hit
ACCGGGTGCTGTAGGCTT271930.2522354790215456No Hit
CCCATAAAGTAGAAAGCACT252840.2345280716206656No Hit
TGATGTAGTAGGTTGTATAGTT244660.22694050784176573No Hit
TGAGGTAGTAGTTTGTACAGTT242360.22480708526334642No Hit
AACCGGGCGGAAACACCA241870.22435257349663973No Hit
CCCCCCACTGCTAAATTTGACTGG236420.21929728956082015No Hit
TGTAAACATCCTACACTCAGCT231860.2150675474053454No Hit
TTCACAGTGGCTAAGTTCCGC218100.2023041149361935No Hit
TAGCAGCACGTAAATATTGGC217880.20210004842869256No Hit
CAACACCAGTCGATGGGCTGT216760.20106116439050575No Hit
CTCCCACTGCTTCACTTGACTAGCCT207640.19260168007955625No Hit
AACATTCAACGCTGTCGGTGAGTT205670.19077435726238845No Hit
GGGCTACGCCTGTCTGAGCGTCGCT201730.1871197116280528No Hit
TAGCAGCACATCATGGTTTACA199490.18504194355167924No Hit
TGAATACCGGGTGCTGTAGGCTTT197480.1831775177331476No Hit
TAGCAGCACGTAAATATTGGCGT194460.1803762411301797No Hit
CACCCGGCTGTGTGCACATGTGC191040.1772039345135736No Hit
CAAAGAATTCTCCTTTTGGGCT184720.1713416602980911No Hit
ACCCCCCACTGCTAAATTTGACTG184430.17107266353820344No Hit
TGAGGTAGTAGTTTGTGCTGTT178400.16547938608260854No Hit
TTCAAGTAATCCAGGATAGGCTT178080.165182562071698No Hit
ATCACATTGCCAGGGATTTCC164540.15262319611004715No Hit
TGTAAACATCCCCGACTGGAAGC164200.1523078205984547No Hit
TTCAAGTAATCCAGGATAGGC163310.15148227881810986No Hit
TGAATACCGGGTGCTGTAGGCTT161310.14962712874991918No Hit
TTCACAGTGGCTAAGTTCCG160240.14863462346343717No Hit
TACGCCTGTCTGAGCGTCGCT156920.14555507435024065No Hit
GTCAGGATGGCCGAGCGGTCTAAGG155470.1442100905508024No Hit
AACGGAATCCCAAAAGCAGCTG150680.13976700613748572No Hit
CAAAGAATTCTCCTTTTGGGCTT150240.13935887312248377No Hit
ACCGGGTGCTGTAGGCTTT149520.13869101909793513No Hit
TGATGTAGTAGATTGTATAGTT143170.13280091763142973No Hit
ATCACATTGCCAGGGATTTCCA142590.13226292411165444No Hit
ACCGGGTGCTGTAGGCTTA136820.1269108161649243No Hit
TTCACAGTGGCTAAGTTCTGC136450.12656761340230904No Hit
CGCGACCTCAGATCAGACGTGGCG136110.12625223789071663No Hit
AGCTCGGTCTGAGGCCCCTCAGT131690.12215235624001522No Hit
CAACGGAATCCCAAAAGCAGCT130760.12128971145830655No Hit
TATTGCACTTGTCCCGGCCTGTT127330.11810812909135955No Hit
TGTCAGTTTGTCAAATACCCC126610.1174402750668109No Hit
AGCAGCATTGTACAGGGCTATGT125610.11651270003271558No Hit
ACGCCTGTCTGAGCGTCGCT125470.11638283952794222No Hit
CCCTGTCTGAGCGTCGCT119600.11093797407780259No Hit
TCGTACGACTCTTAGCGG119140.11051128956211874No Hit
TAGCAGCACATAATGGTTTGT116710.10825728222926706No Hit
CAAAGTGCTGTTCGTGCAGGTAG116360.10793263096733369No Hit
TGAGGTAGTAGGTTGTGTGGTT114990.10666185317062307No Hit
AGCAGCATTGTACAGGGCTATG114010.10575282963720964No Hit
TCCCACTGCTTCACTTGACTAG108980.10108712721571009No Hit
TTATAAAGCAATGAGACTGATT108740.1008645092075272No Hit
ACCACAGGGTAGAACCACGGAC108240.10040072169047952No Hit
ATCCCACCGCTGCCACCA108230.10039144594013857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)