FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10655157
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7182956.741289687237832No Hit
CAACGGAATCCCAAAAGCAGCTG6936606.510087087407534No Hit
AAGCTGCCAGTTGAAGAACTGT3485083.2707917865499305No Hit
AACATTCAACGCTGTCGGTGAGT3434113.2229557950201952No Hit
TAGCAGCACGTAAATATTGGCG3260603.060114459129978No Hit
TAGCTTATCAGACTGATGTTGAC3053512.8657578672937434No Hit
TTCAAGTAATCCAGGATAGGCT2985982.8023801057084374No Hit
CCCCCACTGCTAAATTTGACTG2929332.7492133621306567No Hit
TAGCTTATCAGACTGATGTTGACT2294012.152957483404515No Hit
AACATTCAACGCTGTCGGTGAG1747721.6402573889807537No Hit
TAGCTTATCAGACTGATGTTGA1538491.4438923799996566No Hit
TATTGCACTTGTCCCGGCCTGTAA1274761.1963784297124858No Hit
TGTAAACATCCTTGACTGGAAGCT1088271.0213551991772623No Hit
TATTGCACTTGTCCCGGCCTGTAT1074011.0079720082960768No Hit
TGAGGTAGTAGGTTGTATAGTT863660.8105558651083226No Hit
TATTGCACTTGTCCCGGCCTGT815440.7653007834610039No Hit
AGCAGCATTGTACAGGGCTATGA777950.7301159429185323No Hit
CATAAAGTAGAAAGCACTACT684520.6424307028042853No Hit
TGTAAACATCCCCGACTGGAAGCT608560.5711412792885173No Hit
TTCAAGTAATTCAGGATAGGTT556480.5222635386789701No Hit
TGAGGTAGTAGATTGTATAGTT542030.5087020303877268No Hit
TATTGCACTTGTCCCGGCCTGTA513300.4817385609616076No Hit
CAACGGAATCCCAAAAGCAGCTGT482510.4528417554053873No Hit
ACTGGACTTGGAGTCAGAAGGC469720.44083817817043897No Hit
CCCATAAAGTAGAAAGCACTAC402820.37805167957637786No Hit
TAGCTTATCAGACTGATGTTGACA402600.3778452067857846No Hit
CGCCTGTCTGAGCGTCGCT399860.37527368203021316No Hit
TGTAAACATCCTACACTCTCAGCT382050.35855877111899903No Hit
CATTGCACTTGTCTCGGTCTGA345040.32382441666509465No Hit
ACCGGGTGCTGTAGGCTT323810.3038997923728388No Hit
CCCCCACTGCTAAATTTGACTGGCTT298060.27973309074657465No Hit
CTTCTCACTACTGCACTTGACTAG291540.2736139880435361No Hit
AACATTCAACGCTGTCGGTGAGTT291060.27316350195496886No Hit
TTCTCACTACTGCACTTGACTAG288770.2710143079074292No Hit
CCCATAAAGTAGAAAGCACT278890.2617418025844199No Hit
TCGTACGACTCTTAGCGG259820.24384436569071674No Hit
CTCCCACTGCTTCACTTGACTAG252910.2373592430407173No Hit
CAACGGAATCCCAAAAGCAGCT250120.2347407926509201No Hit
TGAGGTAGTAGTTTGTACAGTT232550.21825112478398956No Hit
CAACACCAGTCGATGGGCTGT228940.21486309399289003No Hit
CCCCCCACTGCTAAATTTGACTGGCT228380.21433752688956156No Hit
CACCCGGCTGTGTGCACATGTGC227060.2130986901460016No Hit
CTACGCCTGTCTGAGCGTCGCT222760.2090630856025866No Hit
TTCAAGTAATCCAGGATAGGCTT217540.20416404938941773No Hit
CCCCCCACTGCTAAATTTGACTGG194250.18230608896706074No Hit
CGCGACCTCAGATCAGACGTGGCG193600.1816960557221259No Hit
ACCGGGTGCTGTAGGCTTT188640.17704103280693095No Hit
ACCGGGTGCTGTAGGCTTA186940.17544556124325525No Hit
ACCCCCCACTGCTAAATTTGACTG186780.17529539921373286No Hit
TGATGTAGTAGGTTGTATAGTT185430.17402840708963743No Hit
TTAATGCTAATCGTGATAGGGGTT184630.17327759694202535No Hit
CAAAGAATTCTCCTTTTGGGCT183460.17217953710114267No Hit
CAAAGAATTCTCCTTTTGGGCTT182250.17104393675287938No Hit
TAGCAGCACGTAAATATTGGC180220.16913875600331368No Hit
GGAATACCGGGTGCTGTAGGCTTT179910.16884781707111401No Hit
TACGCCTGTCTGAGCGTCGCT177120.16622936668131685No Hit
CTCCCACTGCTTCACTTGACTAGCCT169900.15945330509911773No Hit
TGTCAGTTTGTCAAATACCCCA165640.15545524106308334No Hit
TGAGGTAGTAGTTTGTGCTGTT164850.1547138160423164No Hit
AACGGAATCCCAAAAGCAGCTG158510.14876364562249061No Hit
GGAATACCGGGTGCTGTAGGCTT156070.1464736746722737No Hit
TGTAAACATCCCCGACTGGAAGC153060.14364875149188325No Hit
AGCTCGGTCTGAGGCCCCTCAGT151840.14250376601677478No Hit
AGCAGCATTGTACAGGGCTATGT150050.14082382831149273No Hit
TATTGCACTTGTCCCGGCCTGTT144350.13547430600975657No Hit
TATTGCACTTGTCCCGGCCTGTAG138950.130406337513375No Hit
TGTAAACATCCTACACTCAGCT138490.12997462167849802No Hit
TTATAAAGCAATGAGACTGATT137590.12912996026243442No Hit
CGCGACCTCAGATCAGACGTGG136950.12852931214434477No Hit
TTTAAACATCCTTGACTGGAAGCT135650.1273092456544751No Hit
TAGCAGCACGTAAATATTGGCGT133210.12501927470425822No Hit
TGTCAGTTTGTCAAATACCCCAA130500.12247590532922228No Hit
ACCACAGGGTAGAACCACGGAC129280.12133091985411383No Hit
CAAAGTGCTGTTCGTGCAGGTAG117970.1107163413922479No Hit
TTCAAGTAATCCAGGATAGGC117690.11045355784058367No Hit
ACGCCTGTCTGAGCGTCGCT116620.10944934926815252No Hit
TGATGTAGTAGATTGTATAGTT114690.10763801978703832No Hit
ACTGGACTTGGAGTCAGAAGGCA114350.1073189254743032No Hit
CACTAGATTGTGAGCTCCTGGA111870.10499141401670571No Hit
TGCAAGTAATCCAGGATAGGCT111760.10488817762140906No Hit
CACTAGATTGTGAGCTCCTGGAA111420.10456908330867391No Hit
GCGTTCGCGCTTTCCCCTG109280.10256066616381157No Hit
TATTGCACTTGTCCCGGCCTGTAAT109200.10248558514905036No Hit
GGGCTACGCCTGTCTGAGCGTCGCT108090.10144383606923858No Hit
AACATTCAACGCTGTCGGTGA107770.10114351201019375No Hit
AACCGGGCGGAAACACCA107630.10101212023436164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)