FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4205591
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG2417365.7479674081478676No Hit
CAACGGAATCCCAAAAGCAGCTG2101204.99620624069245No Hit
TTCAAGTAATCCAGGATAGGCT1574943.744872004909655No Hit
TAGCAGCACGTAAATATTGGCG1426453.391794399407836No Hit
AACATTCAACGCTGTCGGTGAGT1280113.0438290361568683No Hit
CCCCCACTGCTAAATTTGACTG1180562.8071203310069857No Hit
AAGCTGCCAGTTGAAGAACTGT1160082.75842325133376No Hit
TAGCTTATCAGACTGATGTTGA751211.7862174424474468No Hit
TAGCTTATCAGACTGATGTTGAC729211.7339061263922242No Hit
AACATTCAACGCTGTCGGTGAG727881.7307436695579765No Hit
ACCGGGTGCTGTAGGCTT653381.5535985310982452No Hit
TAGCTTATCAGACTGATGTTGACT625581.487496049901191No Hit
TGAGGTAGTAGGTTGTATAGTT586311.3941203507426185No Hit
CATAAAGTAGAAAGCACTACT533501.26854941433915No Hit
TATTGCACTTGTCCCGGCCTGT446691.062133716759428No Hit
TGAGGTAGTAGATTGTATAGTT430141.0227813403633401No Hit
TATTGCACTTGTCCCGGCCTGTAA402110.9561319681347996No Hit
ACCGGGTGCTGTAGGCTTA397920.9461690402133731No Hit
ACCGGGTGCTGTAGGCTTT375950.893929057770953No Hit
TATTGCACTTGTCCCGGCCTGTAT374830.8912659362263234No Hit
TTCAAGTAATTCAGGATAGGTT337390.8022415874487081No Hit
TGTAAACATCCTTGACTGGAAGCT297340.7070112143572687No Hit
GGAATACCGGGTGCTGTAGGCTTT295050.7015660819133387No Hit
GGAATACCGGGTGCTGTAGGCTT277100.6588848035864638No Hit
GCCTGTCTGAGCGTCGCT251540.5981085654786688No Hit
TTCTCACTACTGCACTTGACTAG238310.5666504422327326No Hit
TATTGCACTTGTCCCGGCCTGTA198430.4718242929471744No Hit
CGCCTGTCTGAGCGTCGCT198080.4709920674644776No Hit
TGAGGTAGTAGTTTGTACAGTT195550.46497626611812704No Hit
CCCATAAAGTAGAAAGCACT187830.4466197497569307No Hit
CATTGCACTTGTCTCGGTCTGA176060.41863319566738655No Hit
AGCAGCATTGTACAGGGCTATGA164790.39183553512455205No Hit
CAACACCAGTCGATGGGCTGT161920.3850112861664389No Hit
TGTAAACATCCCCGACTGGAAGCT158820.37764014617683933No Hit
CTTCTCACTACTGCACTTGACTAG158690.37733103385469485No Hit
ACTGGACTTGGAGTCAGAAGGC136240.3239497136074335No Hit
CTCCCACTGCTTCACTTGACTAG121280.2883780186898821No Hit
CAACGGAATCCCAAAAGCAGCTGT118950.28283777476221533No Hit
ATCCCGGACGAGCCCCCA107960.2567058946055382No Hit
CCCATAAAGTAGAAAGCACTAC100860.23982360624226182No Hit
TGAGGTAGTAGTTTGTGCTGTT100510.23899138075956505No Hit
TGAGAACTGAATTCCATGGGTT97070.23081179315820297No Hit
TGTAAACATCCTACACTCTCAGCT92100.21899419130390949No Hit
AACCGGGCGGAAACACCA89140.21195594150738864No Hit
CACCCGGCTGTGTGCACATGTGC88980.21157549557244154No Hit
CCCCCCACTGCTAAATTTGACTGG83170.19776055255967592No Hit
GAATACCGGGTGCTGTAGGCTTT82050.1950974310150464No Hit
TTCACAGTGGCTAAGTTCCG79540.18912918541056417No Hit
CTACGCCTGTCTGAGCGTCGCT76360.1815678224534911No Hit
AATGACACGATCACTCCCGTTG75630.17983203787529506No Hit
AACGGAATCCCAAAAGCAGCTG75600.17976070426249247No Hit
TTAATGCTAATCGTGATAGGGGTT74230.17650313594450817No Hit
GAATACCGGGTGCTGTAGGCTT74140.17628913510610042No Hit
GGGAATACCGGGTGCTGTAGGCTT72820.17315045614278707No Hit
TTCAAGTAATCCAGGATAGGCTT71980.17115311498431493No Hit
AACATTCAACGCTGTCGGTGAGTT71630.1703208895016182No Hit
TGTCAGTTTGTCAAATACCCCA71440.16986910995386856No Hit
AACATTCAACGCTGTCGGTGA71120.1691082180839744No Hit
TTATAAAGCAATGAGACTGATT70900.16858510492342219No Hit
TTCAAGTAATCCAGGATAGGC70220.16696820969989712No Hit
TAGCTTATCAGACTGATGTTGACA69280.16473308983208304No Hit
CGCGACCTCAGATCAGACGTGG68200.16216507977119032No Hit
TGTCAGTTTGTCAAATACCCCAA68160.16206996828745354No Hit
TAGCAGCACGTAAATATTGGC65640.1560779448120371No Hit
TTCACAGTGGCTAAGTTCTG64120.15246370843003992No Hit
TGAGGTAGTAGGTTGTATAGT64120.15246370843003992No Hit
AGTTCTACAGTCCGACGATC63590.15120348127052774No Hit
CGCGACCTCAGATCAGACGTGGCG62360.14827880314562208No Hit
AGCTCGGTCTGAGGCCCCTCAGT62160.14780324572693826No Hit
TCGTACGACTCTTAGCGG60740.14442678805428297No Hit
TGAGGTAGTAGGTTGTGTGGTT60130.14297633792729725No Hit
TATTGCACTTGTCCCGGCCTGTT59660.14185877799339022No Hit
TACGCCTGTCTGAGCGTCGCT57980.13786409567644595No Hit
CAACGGAATCCCAAAAGCAGCT57910.1376976505799066No Hit
GTTCTACAGTCCGACGATC57670.137126981677486Illumina DpnII expression Sequencing Primer (100% over 19bp)
ATCCCACCGCTGCCACCA57350.13636608980759185No Hit
ATACCGGGTGCTGTAGGCTT56100.13339385594081782No Hit
TGTAAACATCCCCGACTGGAAGC55370.1316580713626218No Hit
TCAGTGCACTACAGAACTTTGT55170.13118251394393798No Hit
TGAGGTAGTAGATTGTATAGT53280.12668849633737564No Hit
GGAATACCGGGTGCTGTAGGCTTA52460.1247387109207719No Hit
TTCACAGTGGCTAAGTTCTGC51290.12195670002147142No Hit
GGGAATACCGGGTGCTGTAGGCTTT51080.1214573647318534No Hit
CAAAGTGCTGTTCGTGCAGGT50710.12057758350728827No Hit
ACTGGACTTGGAGTCAGAAGG48250.11472822725747701No Hit
CCGGGTGCTGTAGGCTTT47850.11377711242010934No Hit
ACGCCTGTCTGAGCGTCGCT47680.11337288861422805No Hit
AGAGGTAGTAGGTTGCATAGTT47640.1132777771304913No Hit
ACCCCCCACTGCTAAATTTGACTG47400.11270710822807069No Hit
CAAAGAATTCTCCTTTTGGGCT46700.11104265726267723No Hit
TATTGCACTTGTCCCGGCCTGTAG46680.11099510152080885No Hit
ATACCGGGTGCTGTAGGCTTA46510.11059087771492758No Hit
CACTAGATTGTGAGCTCCTGGA45260.10761864384815356No Hit
TTCTACAGTCCGACGATC44900.10676264049452265Illumina DpnII expression Sequencing Primer (100% over 18bp)
TGTCAGTTTGTCAAATACC44690.10626330520490462No Hit
TGTAAACATCCTACACTCAGCT43920.10443240914297183No Hit
CAAAGAATTCTCCTTTTGGGCTT43850.10426596404643249No Hit
TGAGAACTGAATTCCATAGGCTGT42970.10217351140422357No Hit
GGAATACCGGGTGCTGTAGGCT42630.10136506379246103No Hit
ATACCGGGTGCTGTAGGCTTT42160.100247503858554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)