FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6941686
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3859265.559542739328745No Hit
CCCCCCACTGCTAAATTTGACTG3041144.380981796065106No Hit
TAGCAGCACGTAAATATTGGCG2528893.643048677223372No Hit
TTCAAGTAATCCAGGATAGGCT2075472.9898644219862436No Hit
AAGCTGCCAGTTGAAGAACTGT2072042.984923259277357No Hit
AACATTCAACGCTGTCGGTGAGT2063522.972649583977149No Hit
TAGCTTATCAGACTGATGTTGAC1542122.2215352293376567No Hit
CCCCCACTGCTAAATTTGACTG1371451.9756727688345455No Hit
TAGCTTATCAGACTGATGTTGACT1086001.564461429111026No Hit
AACATTCAACGCTGTCGGTGAG1018091.4666321697639448No Hit
TAGCTTATCAGACTGATGTTGA994711.4329515912992896No Hit
GCCTGTCTGAGCGTCGCT903551.3016290278759368No Hit
CATAAAGTAGAAAGCACTACT741251.0678241568402835No Hit
TATTGCACTTGTCCCGGCCTGTAA561520.8089101120390636No Hit
TATTGCACTTGTCCCGGCCTGT530490.7642091561041511No Hit
TGAGGTAGTAGGTTGTATAGTT518120.7463892777633561No Hit
TGTAAACATCCTTGACTGGAAGCT501570.7225478075499238No Hit
TATTGCACTTGTCCCGGCCTGTAT474960.6842141808200486No Hit
TCCTGTCTGAGCGTCGCT442520.6374820180572847No Hit
AGCAGCATTGTACAGGGCTATGA390920.5631484915912359No Hit
CGCCTGTCTGAGCGTCGCT342940.49402983655555727No Hit
TTCAAGTAATTCAGGATAGGTT312000.4494585321202947No Hit
CCCATAAAGTAGAAAGCACTAC289770.4174346117067237No Hit
ACTGGACTTGGAGTCAGAAGGC277370.3995715161993787No Hit
TATTGCACTTGTCCCGGCCTGTA276790.39873598431274476No Hit
ACCGGGTGCTGTAGGCTT274720.3957539998207928No Hit
TGTAAACATCCCCGACTGGAAGCT272430.3924550894408073No Hit
CAACGGAATCCCAAAAGCAGCTGT266130.38337948446530135No Hit
CATTGCACTTGTCTCGGTCTGA242600.3494828201678958No Hit
TCGTACGACTCTTAGCGG242440.34925232861296235No Hit
TGAGGTAGTAGATTGTATAGTT242010.3486328825590786No Hit
CCCATAAAGTAGAAAGCACT225910.3254396698438967No Hit
TTCTCACTACTGCACTTGACTAG193630.2789379986360662No Hit
TGTAAACATCCTACACTCTCAGCT191670.276114477088131No Hit
CAACACCAGTCGATGGGCTGT183130.2638119903435563No Hit
AACCGGGCGGAAACACCA177950.25634982625258473No Hit
TAGCTTATCAGACTGATGTTGACA177530.25574478592088434No Hit
CTTCTCACTACTGCACTTGACTAG172460.24844108477392957No Hit
CTACGCCTGTCTGAGCGTCGCT171460.24700051255559527No Hit
AACATTCAACGCTGTCGGTGAGTT170170.24514217439394406No Hit
CTCCCACTGCTTCACTTGACTAG155550.22408100856189692No Hit
TGTCAGTTTGTCAAATACCCCAA149880.21591296408394156No Hit
CCCCCACTGCTAAATTTGACTGGCTT141220.2034376086731667No Hit
ACCGGGTGCTGTAGGCTTT138150.1990150519628805No Hit
GGAATACCGGGTGCTGTAGGCTTT135980.1958890102490951No Hit
TACGCCTGTCTGAGCGTCGCT134610.19391542630997713No Hit
CGCGACCTCAGATCAGACGTGGCG126230.1818434311203359No Hit
CACCCGGCTGTGTGCACATGTGC124010.17864536079563378No Hit
GGAATACCGGGTGCTGTAGGCTT123840.17840046351851696No Hit
CAACGGAATCCCAAAAGCAGCT122180.17600911363608207No Hit
TTCAAGTAATCCAGGATAGGCTT118770.17109676237156218No Hit
TAGCAGCACGTAAATATTGGC117060.16863338387821059No Hit
TGAGGTAGTAGTTTGTACAGTT115030.16570902227499199No Hit
ACCGGGTGCTGTAGGCTTA114930.16556496505315854No Hit
TGATGTAGTAGGTTGTATAGTT113950.16415320427919095No Hit
CCCCCCACTGCTAAATTTGACTGGCT112400.16192031734077283No Hit
ACGCCTGTCTGAGCGTCGCT108040.1556394224688354No Hit
TGTCAGTTTGTCAAATACCCCA107090.15427087886141783No Hit
CAAAGAATTCTCCTTTTGGGCT103310.14882551587611426No Hit
TTCACAGTGGCTAAGTTCCG102750.14801879543384705No Hit
TGTAAACATCCTACACTCAGCT102480.14762984093489678No Hit
TTCAAGTAATCCAGGATAGGC99580.1434521815017274No Hit
CGCGACCTCAGATCAGACGTGG99120.14278951828129363No Hit
GCGTTCGCGCTTTCCCCTG97520.14048460273195879No Hit
TAGCAGCACGTAAATATTGGCGT95420.1374594010734568No Hit
CCCCCCACTGCTAAATTTGACTGG93350.13447741658150483No Hit
AACGGAATCCCAAAAGCAGCTG92940.13388678197198778No Hit
CAAAGAATTCTCCTTTTGGGCTT92600.13339698741775413No Hit
TGAGGTAGTAGTTTGTGCTGTT90850.13087598603566916No Hit
TGTAAACATCCCCGACTGGAAGC90440.13028535142615208No Hit
TTATAAAGCAATGAGACTGATT88140.12697203532398327No Hit
ACCACAGGGTAGAACCACGGAC85280.12285199877954721No Hit
GTCAGGATGGCCGAGCGGTCTAAGG84660.12195884400417996No Hit
GGGCTACGCCTGTCTGAGCGTCGCT82660.11907769956751141No Hit
TATTGCACTTGTCCCGGCCTGTT82550.11891923662349464No Hit
CTCCCACTGCTTCACTTGACTAGCCT79820.11498647446744206No Hit
AGCAGCATTGTACAGGGCTATGT79820.11498647446744206No Hit
AACATTCAACGCTGTCGGTGA78940.1137187709153079No Hit
TTCACAGTGGCTAAGTTCTGC77890.11220617008605689No Hit
ACCCCCCACTGCTAAATTTGACTG76670.11044867197968909No Hit
TGCAAGTAATCCAGGATAGGCT76660.11043426625750574No Hit
TGAATACCGGGTGCTGTAGGCTTT75710.10906572265008817No Hit
CAAAGTGCTGTTCGTGCAGGT74300.10703451582223684No Hit
CCCATAAAGTAGAAAGCACTA73880.10642947549053645No Hit
CACTAGATTGTGAGCTCCTGGA73060.10524820627150235No Hit
ACTGGACTTGGAGTCAGAAGG71880.1035483310538679No Hit
TGAATACCGGGTGCTGTAGGCTT71160.1025111190566672No Hit
TCAGTGCACTACAGAACTTTGT70800.10199251305806688No Hit
AGCTCGGTCTGAGGCCCCTCAGT70480.1015315299481999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)