FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11077242
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG125635211.341740119065738No Hit
CCCCCCACTGCTAAATTTGACTG9420978.504797493816602No Hit
GCCTGTCTGAGCGTCGCT8036647.2550911138350145No Hit
TAGCAGCACGTAAATATTGGCG4077183.6806815270443676No Hit
TTCAAGTAATCCAGGATAGGCT4073503.677359400471706No Hit
AAGCTGCCAGTTGAAGAACTGT3605723.255070169993578No Hit
CCCCCACTGCTAAATTTGACTG3143642.837926624695931No Hit
AACATTCAACGCTGTCGGTGAGT3002622.710620567827262No Hit
AACATTCAACGCTGTCGGTGAG2261752.0417988520969392No Hit
TAGCTTATCAGACTGATGTTGAC1772011.599685192397169No Hit
TGAGGTAGTAGGTTGTATAGTT1758911.5878591439999237No Hit
TAGCTTATCAGACTGATGTTGACT1650701.4901723732315317No Hit
TAGCTTATCAGACTGATGTTGA1369581.2363907911373606No Hit
TATTGCACTTGTCCCGGCCTGTAA1364151.2314888489391131No Hit
CATAAAGTAGAAAGCACTACT1286291.161200594877317No Hit
TATTGCACTTGTCCCGGCCTGTAT1165661.0523016469261932No Hit
TGTAAACATCCTTGACTGGAAGCT1027950.9279836984693483No Hit
TATTGCACTTGTCCCGGCCTGT902680.8148959822309559No Hit
TGAGGTAGTAGATTGTATAGTT838340.756812932316546No Hit
CAACGGAATCCCAAAAGCAGCTGT785280.7089129225487716No Hit
TTCAAGTAATTCAGGATAGGTT653530.5899753747367801No Hit
AGCAGCATTGTACAGGGCTATGA631220.569834982390021No Hit
TGTAAACATCCCCGACTGGAAGCT615690.5558152471526757No Hit
CCCATAAAGTAGAAAGCACTAC614660.5548854128130449No Hit
TCGTACGACTCTTAGCGG584750.5278841068923114No Hit
ACTGGACTTGGAGTCAGAAGGC568700.5133949407262205No Hit
TATTGCACTTGTCCCGGCCTGTA561520.5069131829023867No Hit
CATTGCACTTGTCTCGGTCTGA465990.4206733047810998No Hit
CCCATAAAGTAGAAAGCACT463770.4186691958160705No Hit
CACCCGGCTGTGTGCACATGTGC452480.4084771281515742No Hit
CAACGGAATCCCAAAAGCAGCT417230.37665512769333737No Hit
AACCGGGCGGAAACACCA367330.33160781356947877No Hit
TGTAAACATCCTACACTCTCAGCT357790.3229955615305687No Hit
TGAGGTAGTAGTTTGTACAGTT355410.3208470122797714No Hit
CAACACCAGTCGATGGGCTGT351250.31709156484980644No Hit
TAGCAGCACGTAAATATTGGC314230.28367169373026246No Hit
AACGGAATCCCAAAAGCAGCTG289300.26116609170405414No Hit
CAAAGAATTCTCCTTTTGGGCT275540.2487442271280162No Hit
TTCACAGTGGCTAAGTTCCGC263990.2383174439991471No Hit
TTCTCACTACTGCACTTGACTAG258550.23340647428303904No Hit
TGATGTAGTAGGTTGTATAGTT241090.21764442809861878No Hit
CTTCTCACTACTGCACTTGACTAG240680.21727429986633856No Hit
CGCCTGTCTGAGCGTCGCT240530.21713888709843118No Hit
CCCCCACTGCTAAATTTGACTGGCTT221320.1997970252884247No Hit
AACATTCAACGCTGTCGGTGAGTT219230.1979102740555817No Hit
TGAGGTAGTAGTTTGTGCTGTT211240.19069728728504803No Hit
TAGCTTATCAGACTGATGTTGACA209560.18918066428448524No Hit
CCCCCCACTGCTAAATTTGACTGG205810.18579534508680048No Hit
CTCCCACTGCTTCACTTGACTAG203620.1838183186753526No Hit
CAAAGAATTCTCCTTTTGGGCTT191710.17306654490350576No Hit
CCCATAAAGTAGAAAGCACTA179190.1617640925421689No Hit
CCCCCCACTGCTAAATTTGACTGGCT175310.15826141561229773No Hit
TGTAAACATCCCCGACTGGAAGC174480.15751213162987682No Hit
ACCCCCCACTGCTAAATTTGACTG173930.15701561814754972No Hit
TTCAAGTAATCCAGGATAGGC169540.15305253780679343No Hit
TATTGCACTTGTCCCGGCCTGTT169500.15301642773535146No Hit
CACTAGATTGTGAGCTCCTGGA168240.15187896048492938No Hit
TTCAAGTAATCCAGGATAGGCTT167950.15161716246697507No Hit
AGCTCGGTCTGAGGCCCCTCAGT165880.14974846626985308No Hit
ATCACATTGCCAGGGATTTCC162360.14657077998295964No Hit
TTCACAGTGGCTAAGTTCTGC160700.14507221201811785No Hit
TGAGGTAGTAGGTTGTGTGGTT159060.14359169908899705No Hit
CTACGCCTGTCTGAGCGTCGCT158780.14333892858890326No Hit
TCGTTTCCCGGCCAATGCACCA156410.1411994068559665No Hit
ACTGGACTTGGAGTCAGAAGGCT151930.13715507885446576No Hit
TTCACAGTGGCTAAGTTCCG150970.13628843713985847No Hit
TGTAAACATCCTACACTCAGCT149250.1347357040678537No Hit
CACTAGATTGTGAGCTCCTGGAA146120.1319100909775195No Hit
TGTCAGTTTGTCAAATACCCCA140850.12715258906503984No Hit
TTATAAAGCAATGAGACTGATT139800.1262046996896881No Hit
TATTGCACTTGTCCCGGCCTGTAG138990.1254734707429882No Hit
AGCAGCATTGTACAGGGCTATGT135010.12188051863451209No Hit
ACTGGACTTGGAGTCAGAAGGCA133440.12046319833041473No Hit
TAGCAGCACGTAAATATTGGCGT131580.1187840800083631No Hit
TGAGGTAGTAGGTTGTATAGT127190.1148209996676068No Hit
TATTGCACTTGTCCCGGCCTGTAAT124950.11279883566685642No Hit
TGAGGTAGTAAGTTGTATTGTT122310.11041557095168636No Hit
TGTCAGTTTGTCAAATACCCCAA120840.10908852582619391No Hit
TGATGTAGTAGATTGTATAGTT120140.10845659957595943No Hit
AGAGGTAGTAGGTTGCATAGTT119460.10784272836144593No Hit
TGAGAACTGAATTCCATAGGCTGT118410.10689483898609418No Hit
ACTGGACTTGGAGTCAGAAGG117040.1056580690392067No Hit
CTCCCACTGCTTCACTTGACTAGCCT115300.10408728093148095No Hit
ACCACAGGGTAGAACCACGGAC113400.10237205253798734No Hit
GGAATACCGGGTGCTGTAGGCTTT113370.10234496998440587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)