FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6913632
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG70819810.243501534359943No Hit
CCCCCCACTGCTAAATTTGACTG5795768.38309010372551No Hit
TTCAAGTAATCCAGGATAGGCT2700353.9058341548986117No Hit
TAGCAGCACGTAAATATTGGCG2464043.5640311778237543No Hit
AAGCTGCCAGTTGAAGAACTGT2322863.359825920731679No Hit
CCCCCACTGCTAAATTTGACTG1619762.342849604954386No Hit
GCCTGTCTGAGCGTCGCT1600782.3153965961740517No Hit
GGAATACCGGGTGCTGTAGGCTTT1428022.0655134667277633No Hit
TAGCTTATCAGACTGATGTTGAC1368161.9789309005744014No Hit
AACATTCAACGCTGTCGGTGAGT1282601.8551753984013035No Hit
TAGCTTATCAGACTGATGTTGACT1205161.7431648083091493No Hit
AACATTCAACGCTGTCGGTGAG1180831.7079734645986364No Hit
TATTGCACTTGTCCCGGCCTGTAA985561.4255314717358403No Hit
TGTAAACATCCTTGACTGGAAGCT920101.33084896621631No Hit
TGAGGTAGTAGGTTGTATAGTT865641.2520770558803245No Hit
TATTGCACTTGTCCCGGCCTGTAT865461.251816700686412No Hit
TAGCTTATCAGACTGATGTTGA840501.2157141137972054No Hit
GGAATACCGGGTGCTGTAGGCTT790671.143639117615748No Hit
CATAAAGTAGAAAGCACTACT782331.1315759936311334No Hit
TGAGGTAGTAGATTGTATAGTT567860.821362780084332No Hit
TATTGCACTTGTCCCGGCCTGT529300.7655889118772882No Hit
TTCAAGTAATTCAGGATAGGTT505810.7316125590717006No Hit
CCCATAAAGTAGAAAGCACTAC461840.6680135708698409No Hit
CAACGGAATCCCAAAAGCAGCTGT459370.6644409190422632No Hit
AGCAGCATTGTACAGGGCTATGA404720.5853941893349255No Hit
TGTAAACATCCCCGACTGGAAGCT399950.578494776696243No Hit
TATTGCACTTGTCCCGGCCTGTA398070.5757755113376009No Hit
TGTAAACATCCTACACTCTCAGCT282120.4080633739256009No Hit
ACTGGACTTGGAGTCAGAAGGC277710.4016846716747435No Hit
TTAATGCTAATCGTGATAGGGGTT244630.3538371727045929No Hit
CAAAGAATTCTCCTTTTGGGCT242750.3511179073459507No Hit
CCCCCACTGCTAAATTTGACTGGCTT235680.3408917338961634No Hit
CATTGCACTTGTCTCGGTCTGA233700.33802782676312537No Hit
GGAATACCGGGTGCTGTAGGCTTA232610.3364512314222105No Hit
TTCTCACTACTGCACTTGACTAG224530.324764176051025No Hit
TGAGGTAGTAGTTTGTACAGTT223860.3237950761625727No Hit
CACCCGGCTGTGTGCACATGTGC222460.3217700913210307No Hit
AACCGGGCGGAAACACCA207380.29995811174213494No Hit
TGATGTAGTAGGTTGTATAGTT199220.28815534295143275No Hit
TAGCTTATCAGACTGATGTTGACA198390.28695481622394714No Hit
TAGCAGCACGTAAATATTGGC188330.2724038537197236No Hit
CTCCCACTGCTTCACTTGACTAG186230.2693663764574105No Hit
CCCATAAAGTAGAAAGCACT175330.25360042304826175No Hit
CTTCTCACTACTGCACTTGACTAG170620.24678779547421675No Hit
TGAGAACTGAATTCCATGGGTT167810.24272336161369307No Hit
CAACGGAATCCCAAAAGCAGCT162110.23447878047312903No Hit
TGTCAGTTTGTCAAATACCCCA156210.22594491578377326No Hit
CCCCCCACTGCTAAATTTGACTGGCT145650.21067074440757044No Hit
ACCCCCCACTGCTAAATTTGACTG143560.20764773132269698No Hit
AACGGAATCCCAAAAGCAGCTG135480.19596067595151145No Hit
TTCACAGTGGCTAAGTTCCGC135460.19593174759663226No Hit
TCGTACGACTCTTAGCGG131800.1906378586537438No Hit
TGATGTAGTAGATTGTATAGTT131310.1899291139592041No Hit
TGTCAGTTTGTCAAATACCCCAA130010.18804877089205788No Hit
CAAAGAATTCTCCTTTTGGGCTT128940.18650110390602218No Hit
ATCACATTGCCAGGGATTTCC125830.18200274472231093No Hit
GGAATACCGGGTGCTGTAGGCT124750.18044061355883564No Hit
TTCACAGTGGCTAAGTTCCG123790.17905205252463538No Hit
CCCCCCACTGCTAAATTTGACTGG122690.17746099300628093No Hit
TGAGAACTGAATTCCATAGGCTGT121570.1758410051330473No Hit
CAACACCAGTCGATGGGCTGT121110.17517565297082632No Hit
TGAGGTAGTAGTTTGTGCTGTT120920.1749008335994742No Hit
TTCAAGTAATCCAGGATAGGC116010.16779892247663744No Hit
TTCAAGTAATCCAGGATAGGCTT114140.16509412129543488No Hit
TATTGCACTTGTCCCGGCCTGTT112850.16322824240572828No Hit
AGCTCGGTCTGAGGCCCCTCAGT111980.1619698589684843No Hit
TCGTTTCCCGGCCAATGCACCA110580.15994487412694225No Hit
TGTAAACATCCCCGACTGGAAGC110240.15945309209399633No Hit
CTCCCACTGCTTCACTTGACTAGCCT108660.15716775205854172No Hit
AACATTCAACGCTGTCGGTGAGTT105370.15240903768091793No Hit
TATTGCACTTGTCCCGGCCTGTAG105110.1520329690674887No Hit
TTCACAGTGGCTAAGTTCTGC101580.14692711443131484No Hit
CCCATAAAGTAGAAAGCACTA99650.14413552818547473No Hit
ATCACATTGCCAGGGATTTCCA99160.14342678349093502No Hit
TTATAAAGCAATGAGACTGATT98270.14213947169881186No Hit
TGTAAACATCCTACACTCAGCT97870.14156090460122842No Hit
CACTAGATTGTGAGCTCCTGGAA90220.1304958088599451No Hit
TATTGCACTTGTCCCGGCCTGTAAT89560.12954117314893243No Hit
GGAATACCAGGTGCTGTAGGCTTT89540.12951224479405327No Hit
TAGCAGCACATCATGGTTTACA88710.1283117180665676No Hit
AGCAGCATTGTACAGGGCTATGT87920.12716904804884033No Hit
TATTGCACTTGTCCCGGCCTGTAAA86720.12543334675609No Hit
CACTAGATTGTGAGCTCCTGGA86540.12517299156217743No Hit
TAGCAGCACGTAAATATTGGCGT85600.12381335888285636No Hit
GGGAATACCGGGTGCTGTAGGCTTT84270.12188962328339142No Hit
TATTGCACTTGTCCCGGCCTGTTT79520.11501913899958807No Hit
CTACGCCTGTCTGAGCGTCGCT79040.11432485848248794No Hit
ATGCACCTGGGCAAGGATTCTGA76870.11118613197809775No Hit
CAAAGTGCTGTTCGTGCAGGTAG75640.10940703815302869No Hit
TGTCAGTTTGTCAAATACCCC75430.10910329042679737No Hit
CGCCTGTCTGAGCGTCGCT71110.1028547657728962No Hit
TCGTTTCCCGGGCGGCGCACCA70640.10217494943323567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)