FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6734190
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5561908.259196725961102No Hit
CCCCCCACTGCTAAATTTGACTG3560835.287688645553511No Hit
GCCTGTCTGAGCGTCGCT2618233.8879657390124125No Hit
AAGCTGCCAGTTGAAGAACTGT2445263.6311122792793196No Hit
TTCAAGTAATCCAGGATAGGCT2141683.180308247911033No Hit
TAGCAGCACGTAAATATTGGCG2052023.0471667713563173No Hit
AACATTCAACGCTGTCGGTGAGT1931312.8679172996306903No Hit
CCCCCACTGCTAAATTTGACTG1466832.1781832707422866No Hit
AACATTCAACGCTGTCGGTGAG1464532.1747678636925896No Hit
TGAGGTAGTAGGTTGTATAGTT1088981.6170912908605193No Hit
TAGCTTATCAGACTGATGTTGAC873911.2977210325220998No Hit
TATTGCACTTGTCCCGGCCTGT797921.18487895351928No Hit
CATAAAGTAGAAAGCACTACT775621.1517643547330858No Hit
TATTGCACTTGTCCCGGCCTGTAA743061.103414070586069No Hit
TAGCTTATCAGACTGATGTTGACT726171.0783331031645975No Hit
TAGCTTATCAGACTGATGTTGA719891.0690075569593374No Hit
TATTGCACTTGTCCCGGCCTGTAT654190.9714457121049451No Hit
TGAGGTAGTAGATTGTATAGTT585190.8689835006140308No Hit
TCCTGTCTGAGCGTCGCT553690.8222072736290482No Hit
TGTAAACATCCTTGACTGGAAGCT493380.7326493609476418No Hit
CGCCTGTCTGAGCGTCGCT444240.6596784468510689No Hit
TATTGCACTTGTCCCGGCCTGTA413240.6136446996594987No Hit
TTCAAGTAATTCAGGATAGGTT405950.6028193442715457No Hit
AACCGGGCGGAAACACCA397800.5907169236389231No Hit
AGCAGCATTGTACAGGGCTATGA394990.5865441871999454No Hit
CAACGGAATCCCAAAAGCAGCT391300.5810646863245617No Hit
CCCATAAAGTAGAAAGCACTAC330050.49011091163153997No Hit
ACTGGACTTGGAGTCAGAAGGC326940.4854926873165147No Hit
CAACACCAGTCGATGGGCTGT321700.47771149908155247No Hit
CCCATAAAGTAGAAAGCACT321310.4771323648426908No Hit
CAACGGAATCCCAAAAGCAGCTGT310950.46174818352318536No Hit
CATTGCACTTGTCTCGGTCTGA301960.44839839683763005No Hit
TCGTACGACTCTTAGCGG297600.44192397303907377No Hit
TGTAAACATCCCCGACTGGAAGCT294520.4373502975116532No Hit
TGAGGTAGTAGTTTGTACAGTT278480.4135315457389827No Hit
CACCCGGCTGTGTGCACATGTGC264870.39332124576229655No Hit
TCGTTTCCCGGCCAATGCACCA209640.3113069277819604No Hit
ATCCCGGACGAGCCCCCA196810.29225489628299767No Hit
TGAGAACTGAATTCCATGGGTT182220.2705893359112232No Hit
TTAATGCTAATCGTGATAGGGGTT176530.2621399158621898No Hit
CTACGCCTGTCTGAGCGTCGCT167060.24807734857495856No Hit
ACCGGGTGCTGTAGGCTT166850.2477655070617253No Hit
AACGGAATCCCAAAAGCAGCTG166320.24697847848070814No Hit
TGTAAACATCCTACACTCTCAGCT162210.24087529457885803No Hit
TGAGGTAGTAGTTTGTGCTGTT161880.24038525791520585No Hit
TGATGTAGTAGGTTGTATAGTT160050.23766778187131637No Hit
TAGCAGCACGTAAATATTGGC149730.222342998935284No Hit
TGAGAACTGAATTCCATAGGCTGT143880.21365598535235866No Hit
AACATTCAACGCTGTCGGTGAGTT127240.18894625782759322No Hit
TTCACAGTGGCTAAGTTCCGC125310.18608028582502129No Hit
TACGCCTGTCTGAGCGTCGCT122850.18242728524143215No Hit
TATTGCACTTGTCCCGGCCTGTT122700.18220454130340843No Hit
TGAGGTAGTAGGTTGTGTGGTT118780.17638349972305506No Hit
ACGCCTGTCTGAGCGTCGCT117980.17519553205359517No Hit
ACCGGGTGCTGTAGGCTTT114910.1706367061220429No Hit
TTCTCACTACTGCACTTGACTAG111930.16621152655330484No Hit
TTCACAGTGGCTAAGTTCTGC111120.16500870928797673No Hit
TTCACAGTGGCTAAGTTCCG106850.15866793185223466No Hit
TTATAAAGCAATGAGACTGATT102760.15259444714212103No Hit
TGAGGTAGTAGGTTGTATAGT99680.1480207716147005No Hit
TTCAAGTAATCCAGGATAGGC95780.14222942922608361No Hit
TTCAAGTAATCCAGGATAGGCTT93930.13948225399045766No Hit
TAGCTTATCAGACTGATGTTGACA93770.13924466045656567No Hit
AGCTCGGTCTGAGGCCCCTCAGT93200.13839823349207553No Hit
ACCGGGTGCTGTAGGCTTA93180.13836853430033902No Hit
CCCTGTCTGAGCGTCGCT92730.13770030248626786No Hit
AGAGGTAGTAGGTTGCATAGTT91770.13627474128291597No Hit
CAACGGAATCCCAAAAGCAGCTGA90400.13424034664896595No Hit
AGCAGCATTGTACAGGGCTATGT89700.13320087493818855No Hit
CACTAGATTGTGAGCTCCTGGA89230.13250294393238088No Hit
TGTAAACATCCCCGACTGGAAGC88640.13162681777615423No Hit
CTCCCACTGCTTCACTTGACTAG87490.12991911425130565No Hit
AATGCACCCGGGCAAGGATTCT87380.12975576869675493No Hit
ACCACAGGGTAGAACCACGGAC87070.12929543122483922No Hit
TGATGTAGTAGATTGTATAGTT85480.12693434548178772No Hit
ACTGGACTTGGAGTCAGAAGGCT85260.12660765437268626No Hit
AGCAGCATTGTACAGGGCTATG81150.12050447047083612No Hit
ACTGGACTTGGAGTCAGAAGG80890.12011838097826168No Hit
CAAAGAATTCTCCTTTTGGGCT80590.11967289310221421No Hit
CCCATAAAGTAGAAAGCACTA80200.11909375886335254No Hit
TATTGCACTTGTCCCGGCCTGTAG79770.11845522624101786No Hit
CCCCCCACTGCTAAATTTTACTG77800.11552985585497291No Hit
TTCACAGTGGCTAAGTTCTG77430.1149804208078477No Hit
CTTCTCACTACTGCACTTGACTAG76810.11405974586401631No Hit
CAAAGTGCTGTTCGTGCAGGT76750.11397064828880682No Hit
ATCCCACCGCTGCCACCA76680.11386670111772908No Hit
TAGCAGCACGTAAATATTGGCGT74780.11104527790276188No Hit
ACTGGACTTGGAGTCAGAAGGCA73810.10960486710354178No Hit
TGTAAACATCCTACACTCAGCT71810.10663494792989209No Hit
TGAGGTAGTAAGTTGTATTGTT70670.10494209400091176No Hit
CCCCCCACTGCTAAATTTGACTGG70280.10436295976205007No Hit
AATGACACGATCACTCCCGTTG69210.10277405300414749No Hit
CAAAGAATTCTCCTTTTGGGCTT68610.10188307725205259No Hit
CCCCCACTGCTAAATTTGACTGGCTT67670.10048721524043723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)