FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7389281
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5662467.663073037823301No Hit
CCCCCCACTGCTAAATTTGACTG5563907.529690642431923No Hit
GCCTGTCTGAGCGTCGCT3954965.352293409873031No Hit
AAGCTGCCAGTTGAAGAACTGT2468933.341231711177312No Hit
AACATTCAACGCTGTCGGTGAGT2383953.2262272878782117No Hit
TTCAAGTAATCCAGGATAGGCT2318843.138113166896752No Hit
TAGCAGCACGTAAATATTGGCG2149592.9090651715640536No Hit
CCCCCACTGCTAAATTTGACTG1878282.5418981900945434No Hit
AACATTCAACGCTGTCGGTGAG1620652.193244511881467No Hit
TCCTGTCTGAGCGTCGCT1290701.7467193357513404No Hit
TAGCTTATCAGACTGATGTTGAC1165831.5777313110707254No Hit
TGAGGTAGTAGGTTGTATAGTT1102231.4916606906680094No Hit
TAGCTTATCAGACTGATGTTGA861301.1656073168688537No Hit
TAGCTTATCAGACTGATGTTGACT859571.1632660877289684No Hit
TATTGCACTTGTCCCGGCCTGTAA789831.0688861338471225No Hit
TATTGCACTTGTCCCGGCCTGT744701.0078111794638749No Hit
CATAAAGTAGAAAGCACTACT688080.9311866743191929No Hit
TATTGCACTTGTCCCGGCCTGTAT681390.9221330194372092No Hit
TGAGGTAGTAGATTGTATAGTT661650.8954186476329699No Hit
TGTAAACATCCTTGACTGGAAGCT532420.7205301841951877No Hit
TATTGCACTTGTCCCGGCCTGTA479670.6491429951033124No Hit
TTCAAGTAATTCAGGATAGGTT463860.6277471380503732No Hit
TTAATGCTAATCGTGATAGGGGTT417700.5652782726763267No Hit
AGCAGCATTGTACAGGGCTATGA399140.5401608086091191No Hit
AACCGGGCGGAAACACCA387440.524327062402959No Hit
CGCCTGTCTGAGCGTCGCT381180.5158553315268427No Hit
ACTGGACTTGGAGTCAGAAGGC362870.4910761953700231No Hit
TGTAAACATCCCCGACTGGAAGCT300550.4067378138684941No Hit
CAACGGAATCCCAAAAGCAGCTGT287800.3894830904387044No Hit
CCCATAAAGTAGAAAGCACT279270.3779393421362647No Hit
CATTGCACTTGTCTCGGTCTGA273900.37067205862112973No Hit
TGAGGTAGTAGTTTGTACAGTT273680.37037433005998827No Hit
TCGTACGACTCTTAGCGG269620.3648798847952866No Hit
CCCATAAAGTAGAAAGCACTAC268450.3632965101746706No Hit
CAACACCAGTCGATGGGCTGT258410.3497092612934872No Hit
CAACGGAATCCCAAAAGCAGCT232200.31423896316840566No Hit
AACATTCAACGCTGTCGGTGAGTT205730.2784168040165207No Hit
TTCACAGTGGCTAAGTTCCG194300.26294845195357974No Hit
CACCCGGCTGTGTGCACATGTGC192600.26064782216294113No Hit
TGTAAACATCCTACACTCTCAGCT189350.2562495593278967No Hit
TGAGGTAGTAGTTTGTGCTGTT170850.23121329395918225No Hit
TTCTCACTACTGCACTTGACTAG157890.21367437508466655No Hit
AACGGAATCCCAAAAGCAGCTG156540.21184740436857116No Hit
ACCGGGTGCTGTAGGCTT145070.1963249198399682No Hit
TAGCAGCACGTAAATATTGGC144900.19609485686090433No Hit
CTACGCCTGTCTGAGCGTCGCT136790.18511949944791653No Hit
TTATAAAGCAATGAGACTGATT134190.181600889179881No Hit
TATTGCACTTGTCCCGGCCTGTT131300.1776898185357953No Hit
CAAAGAATTCTCCTTTTGGGCT130230.1762417750793345No Hit
TACGCCTGTCTGAGCGTCGCT126790.17158638303239518No Hit
TGATGTAGTAGGTTGTATAGTT122100.16523935143351565No Hit
ATCCCGGACGAGCCCCCA122070.1651987520842691No Hit
CTTCTCACTACTGCACTTGACTAG119500.1617207411654801No Hit
TAGCTTATCAGACTGATGTTGACA117330.158784054903312No Hit
AGCTCGGTCTGAGGCCCCTCAGT117030.15837806141084634No Hit
TTCACAGTGGCTAAGTTCTG115900.15684881925589242No Hit
GGAATACCGGGTGCTGTAGGCTTT114740.15527897775169194No Hit
CTCCCACTGCTTCACTTGACTAG109090.14763276697692237No Hit
ACCGGGTGCTGTAGGCTTT109020.14753803516201375No Hit
TTCAAGTAATCCAGGATAGGC106440.14404649112680923No Hit
ACTGGACTTGGAGTCAGAAGG105580.14288264311507437No Hit
CACTAGATTGTGAGCTCCTGGA105130.14227365287637594No Hit
TGAGGTAGTAGGTTGTATAGT103320.13982415880516658No Hit
CCCTGTCTGAGCGTCGCT102420.13860617832776964No Hit
TGAGGTAGTAGGTTGTGTGGTT101270.1370498699399847No Hit
CCCCCCACTGCTAAATTTGACTGG100580.13611608490731372No Hit
TCGTTTCCCGGCCAATGCACCA100100.1354664953193687No Hit
GGAATACCGGGTGCTGTAGGCTT99520.13468157456726845No Hit
TTCAAGTAATCCAGGATAGGCTT98640.13349066032270257No Hit
TGTCAGTTTGTCAAATACCCCA97500.13194788505133315No Hit
ACGCCTGTCTGAGCGTCGCT96020.12994498382183597No Hit
TGTCAGTTTGTCAAATACCCCAA95390.12909239748765813No Hit
ACTGGACTTGGAGTCAGAAGGCA94820.12832100985197342No Hit
ACCGGGTGCTGTAGGCTTA94490.12787441701026123No Hit
AGCTACATTGTCTGCTGGGTTT93090.12597978071208824No Hit
CAACGGAATCCCAAAAGCAGCTGA91060.1232325580797374No Hit
ACTGGACTTGGAGTCAGAAGGCT89880.12163565034270588No Hit
CCCATAAAGTAGAAAGCACTA89840.1215815178770438No Hit
TATTGCACTTGTCCCGGCCTGTAG89180.12068833219361938No Hit
TGAGAACTGAATTCCATGGGTT88860.1202552724683227No Hit
AGAGGTAGTAGGTTGCATAGTT84670.11458489669021925No Hit
AGCAGCATTGTACAGGGCTATG84620.11451723110814163No Hit
ACCCCCCACTGCTAAATTTGACTG83600.11313685323375847No Hit
TAGCAGCACGTAAATATTGGCGT81760.11064675981330253No Hit
CACTAGATTGTGAGCTCCTGGAA81680.11053849488197837No Hit
TTCACAGTGGCTAAGTTCTGC81660.11051142864914733No Hit
CCCCCACTGCTAAATTTGACTGGCTT81140.10980770659554022No Hit
TGAGAACTGAATTCCATAGGCTGT80960.10956411050006083No Hit
CAAAGAATTCTCCTTTTGGGCTT80810.10936111375382802No Hit
ACCACAGGGTAGAACCACGGAC78760.10658682488864614No Hit
AGCAGCATTGTACAGGGCTATGT78390.10608609958127184No Hit
TGTAAACATCCTACACTCAGCT75630.10235095945058796No Hit
TGAGGTAGTAAGTTGTATTGTT74950.1014307075343325No Hit
AATGACACGATCACTCCCGTTG74600.10095704845978926No Hit
TGTAAACATCCCCGACTGGAAGC74300.10055105496732362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)