FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8334302
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5256936.307582806574564No Hit
CCCCCCACTGCTAAATTTGACTG4136124.962767127949047No Hit
TTCAAGTAATCCAGGATAGGCT2979503.5749844438082516No Hit
TAGCAGCACGTAAATATTGGCG2773353.327633195917307No Hit
AACATTCAACGCTGTCGGTGAGT2705313.245994685577749No Hit
AAGCTGCCAGTTGAAGAACTGT2627173.1522375839032475No Hit
TAGCTTATCAGACTGATGTTGAC2020412.4242102098052123No Hit
GCCTGTCTGAGCGTCGCT1751232.101231752821052No Hit
AACATTCAACGCTGTCGGTGAG1451551.74165754972642No Hit
TAGCTTATCAGACTGATGTTGACT1304241.5649060953154805No Hit
CCCCCACTGCTAAATTTGACTG1301161.561210524888587No Hit
TAGCTTATCAGACTGATGTTGA1143161.3716325614310592No Hit
TATTGCACTTGTCCCGGCCTGTAA992071.190345634223478No Hit
TGAGGTAGTAGGTTGTATAGTT979631.175419369252518No Hit
TGTAAACATCCTTGACTGGAAGCT969321.1630488072066503No Hit
TATTGCACTTGTCCCGGCCTGTAT825100.9900049218278867No Hit
TATTGCACTTGTCCCGGCCTGT823810.988457101746493No Hit
TTCAAGTAATTCAGGATAGGTT818710.9823378130526108No Hit
CATAAAGTAGAAAGCACTACT796970.9562528451692776No Hit
AGCAGCATTGTACAGGGCTATGA666690.799935015553792No Hit
TGAGGTAGTAGATTGTATAGTT656980.7882843698248515No Hit
TCCTGTCTGAGCGTCGCT478980.5747091958030798No Hit
TATTGCACTTGTCCCGGCCTGTA466700.559974908516634No Hit
TGTAAACATCCCCGACTGGAAGCT465230.5582111135401622No Hit
ACTGGACTTGGAGTCAGAAGGC444610.5334699894484265No Hit
CGCCTGTCTGAGCGTCGCT438220.5258028806731506No Hit
TCGTACGACTCTTAGCGG427180.5125564204416878No Hit
TGTAAACATCCTACACTCTCAGCT381950.45828672875065No Hit
AACCGGGCGGAAACACCA363710.4364012727160595No Hit
CATTGCACTTGTCTCGGTCTGA351560.4218229672982812No Hit
CAACGGAATCCCAAAAGCAGCTGT339360.40718466885409244No Hit
CCCATAAAGTAGAAAGCACTAC336400.40363308169058426No Hit
TGAGGTAGTAGTTTGTACAGTT257760.30927604975197687No Hit
CCCATAAAGTAGAAAGCACT254580.30546049327226205No Hit
AACATTCAACGCTGTCGGTGAGTT254240.3050525406926699No Hit
TTCTCACTACTGCACTTGACTAG248950.2987052784984273No Hit
CTCCCACTGCTTCACTTGACTAG244950.29390583638557854No Hit
TGATGTAGTAGGTTGTATAGTT244950.29390583638557854No Hit
TAGCTTATCAGACTGATGTTGACA232090.27847562999276965No Hit
CCCCCACTGCTAAATTTGACTGGCTT208730.2504468880537326No Hit
CTACGCCTGTCTGAGCGTCGCT207920.24947500102588077No Hit
CACCCGGCTGTGTGCACATGTGC205600.24669132460042845No Hit
TTCACAGTGGCTAAGTTCCG199480.23934817816776977No Hit
TGTCAGTTTGTCAAATACCCCA189730.22764953801770083No Hit
CTTCTCACTACTGCACTTGACTAG180400.216454839289481No Hit
CAAAGAATTCTCCTTTTGGGCT178750.21447506941793087No Hit
TTCAAGTAATCCAGGATAGGCTT178640.2143430847598275No Hit
CCCCCCACTGCTAAATTTGACTGGCT176890.21224332883545616No Hit
TGTCAGTTTGTCAAATACCCCAA176510.21178738183473553No Hit
TGAGGTAGTAGTTTGTGCTGTT174360.20920768169907933No Hit
TGTAAACATCCTACACTCAGCT173230.20785183930219953No Hit
CAACGGAATCCCAAAAGCAGCT168240.20186453526642062No Hit
TTATAAAGCAATGAGACTGATT167100.20049669426425873No Hit
TGATGTAGTAGATTGTATAGTT166610.19990876260543472No Hit
AGAATTACTCTGAGACCT162310.1947493623341223No Hit
CAACACCAGTCGATGGGCTGT162210.19462937628130106No Hit
TTCAAGTAATCCAGGATAGGC161210.19342951575308887No Hit
TAGCAGCACGTAAATATTGGC157630.18913401506208918No Hit
TACGCCTGTCTGAGCGTCGCT153660.18437056876508673No Hit
AGCAGCATTGTACAGGGCTATGT150400.18045902344311496No Hit
GGAATACCGGGTGCTGTAGGCTTT144690.1736078198270233No Hit
TATTGCACTTGTCCCGGCCTGTT139500.16738054368560198No Hit
AGCTCGGTCTGAGGCCCCTCAGT139390.16724855902749863No Hit
CAAAGTGCTGTTCGTGCAGGTAG135590.16268908902029228No Hit
CAAAGAATTCTCCTTTTGGGCTT130730.15685776685318098No Hit
ACGCCTGTCTGAGCGTCGCT125560.1506544879223239No Hit
TAGCAGCACGTAAATATTGGCGT118860.14261542238330216No Hit
TGTAAACATCCCCGACTGGAAGC117930.14149955209206483No Hit
CAAAGTGCTGTTCGTGCAGGT117810.14135556882867936No Hit
TTAATGCTAATCGTGATAGGGGTT117660.14117558974944752No Hit
AACGGAATCCCAAAAGCAGCTG116000.13918382127261528No Hit
GGAATACCGGGTGCTGTAGGCTT115420.1384879021662522No Hit
TCGTTTCCCGGCCAATGCACCA112600.13510429547669378No Hit
ATCACATTGCCAGGGATTTCCA112010.1343963777650486No Hit
AGCAGCATTGTACAGGGCTATG111260.13349648236888945No Hit
GTCAGGATGGCCGAGCGGTCTAAGG110130.13214063997200964No Hit
TTCACAGTGGCTAAGTTCTGC110040.13203265252447055No Hit
ACCACAGGGTAGAACCACGGAC109570.1314687180762108No Hit
TGTCAGTTTGTCAAATACCCC108520.130208864521588No Hit
ACTTGACTTGGAGTCAGAAGGC107810.12935696354655735No Hit
CTCCCACTGCTTCACTTGACTAGCCT107670.12918898307260762No Hit
GCGTTCGCGCTTTCCCCTG104040.12483348935519735No Hit
CCCCCCACTGCTAAATTTGACTGG103830.12458151864427279No Hit
ATCACATTGCCAGGGATTTCC103500.12418556466996276No Hit
TATTGCACTTGTCCCGGCCTGTAG101970.12234977806179809No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC99610.11951810721521729No Hit
TGTAAACATCCTTGACTGGAAGC99080.11888218113526483No Hit
TTCACAGTGGCTAAGTTCTG98460.11813826760777328No Hit
AGAGGTAGTAGGTTGCATAGTT95850.11500663162913943No Hit
TTCACAGTGGCTAAGTTCCGC94940.11391475854846632No Hit
TGAGGTAGTAGGTTGTATAGT94460.11333882549492448No Hit
CACTAGATTGTGAGCTCCTGGA91960.11033917417439397No Hit
TTCAAGTAATCCAGGATAGGCTA91390.10965525367331301No Hit
TGAGGTAGTAGGTTGTGTGGTT91290.1095352676204918No Hit
CACTAGATTGTGAGCTCCTGGAA90160.10817942522361201No Hit
ACCCCCCACTGCTAAATTTGACTG88310.10595968324641943No Hit
AACATTCATTGCTGTCGGTGGGTT84900.10186815884521583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)