FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8166940
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG86208610.555801805817111No Hit
CCCCCCACTGCTAAATTTGACTG6986898.55508917660715No Hit
AAGCTGCCAGTTGAAGAACTGT3130583.8332349692785797No Hit
TAGCAGCACGTAAATATTGGCG2648093.242450660835025No Hit
TTCAAGTAATCCAGGATAGGCT2424442.968602683502022No Hit
AACATTCAACGCTGTCGGTGAGT2109172.5825707057967855No Hit
CCCCCACTGCTAAATTTGACTG2002492.451946506280198No Hit
TAGCTTATCAGACTGATGTTGAC1882912.305526917058286No Hit
TAGCTTATCAGACTGATGTTGACT1353091.6567894462307793No Hit
AACATTCAACGCTGTCGGTGAG1187891.454510502097481No Hit
TATTGCACTTGTCCCGGCCTGTAA1037281.2700962661657855No Hit
TATTGCACTTGTCCCGGCCTGTAT911481.116060605318516No Hit
TGTAAACATCCTTGACTGGAAGCT856471.0487036760402306No Hit
TAGCTTATCAGACTGATGTTGA790300.9676818979936181No Hit
AGCAGCATTGTACAGGGCTATGA714090.8743666538507691No Hit
CAACGGAATCCCAAAAGCAGCTGT608620.7452240373995646No Hit
TGAGGTAGTAGGTTGTATAGTT603390.7388201701004293No Hit
GGAATACCGGGTGCTGTAGGCTTT533190.6528638633319211No Hit
TATTGCACTTGTCCCGGCCTGT532000.6514067692428254No Hit
TGTAAACATCCCCGACTGGAAGCT507010.6208077933718137No Hit
CCCATAAAGTAGAAAGCACTAC434350.5318393425199647No Hit
CATAAAGTAGAAAGCACTACT418950.5129828307787249No Hit
TATTGCACTTGTCCCGGCCTGTA372840.45652349594829883No Hit
TTCAAGTAATTCAGGATAGGTT362820.4442545188283494No Hit
TGTAAACATCCTACACTCTCAGCT354230.4337365035129437No Hit
TGAGGTAGTAGATTGTATAGTT341040.41758602365145325No Hit
CCCCCACTGCTAAATTTGACTGGCTT329380.40330895047594323No Hit
ACTGGACTTGGAGTCAGAAGGC329190.4030763052012136No Hit
TAGCTTATCAGACTGATGTTGACA291130.3564737833264356No Hit
GGAATACCGGGTGCTGTAGGCTT283350.34694757155066647No Hit
CATTGCACTTGTCTCGGTCTGA250300.3064795382358631No Hit
CCCCCCACTGCTAAATTTGACTGGCT250170.3063203598899955No Hit
TTCTCACTACTGCACTTGACTAG232650.28486801666229944No Hit
CAACGGAATCCCAAAAGCAGCT227340.2783661934580149No Hit
TGATGTAGTAGGTTGTATAGTT222940.27297861867480355No Hit
CAAAGAATTCTCCTTTTGGGCT218310.267309420664288No Hit
ACCCCCCACTGCTAAATTTGACTG203980.24976306915442015No Hit
TGAATACCGGGTGCTGTAGGCTTT201580.24682439199994125No Hit
CTTCTCACTACTGCACTTGACTAG200190.2451224081479722No Hit
CTCCCACTGCTTCACTTGACTAG192070.23517988377531854No Hit
AACATTCAACGCTGTCGGTGAGTT187830.2299882208024058No Hit
CCCATAAAGTAGAAAGCACT168990.20691960513974633No Hit
AGCTCGGTCTGAGGCCCCTCAGT165260.20235241106216034No Hit
CCCCCCACTGCTAAATTTGACTGG164210.20106673980707584No Hit
CACCCGGCTGTGTGCACATGTGC159440.19522611896254902No Hit
TAGCAGCACGTAAATATTGGC159210.19494449573524478No Hit
TTCAAGTAATCCAGGATAGGCTT155970.19097728157669824No Hit
CTCCCACTGCTTCACTTGACTAGCCT148120.18136535838392348No Hit
CAAAGAATTCTCCTTTTGGGCTT143950.17625940682801639No Hit
TGTAAACATCCCCGACTGGAAGC136730.1674188863882923No Hit
ACTTGACTTGGAGTCAGAAGGC136360.1669658403269768No Hit
TGTAAACATCCTACACTCAGCT135040.1653495678920134No Hit
TGAGGTAGTAGTTTGTACAGTT134000.16407614112507254No Hit
AACGGAATCCCAAAAGCAGCTG128080.15682740414402457No Hit
TGTCAGTTTGTCAAATACCCCA127360.1559458009976809No Hit
TGATGTAGTAGATTGTATAGTT126980.15548051044822175No Hit
TGTCAGTTTGTCAAATACCCCAA122890.15047251479746393No Hit
CACTAGATTGTGAGCTCCTGGAA121230.148439929765616No Hit
AGCAGCATTGTACAGGGCTATGT119670.14652978961520471No Hit
ATCACATTGCCAGGGATTTCCA116780.14299113254168636No Hit
CAAAGTGCTGTTCGTGCAGGTAG114720.14046876798409197No Hit
TAGCAGCACGTAAATATTGGCGT111130.13607299674051726No Hit
TATTGCACTTGTCCCGGCCTGTT110860.13574239556063836No Hit
TATTGCACTTGTCCCGGCCTGTAG109700.13432203493597358No Hit
CACTAGATTGTGAGCTCCTGGA108400.1327302514772975No Hit
ATCACATTGCCAGGGATTTCC108330.13264454006029186No Hit
TGAATACCGGGTGCTGTAGGCTT106990.13100377864904114No Hit
TATTGCACTTGTCCCGGCCTGTAAT103940.12726920976522418No Hit
AAGCTGCCAGTTGAAGCACTGT101300.12403666489529737No Hit
TGAGGTAGTAGTTTGTGCTGTT99690.12206530230416779No Hit
CTACGCCTGTCTGAGCGTCGCT99470.12179592356500722No Hit
CGCGACCTCAGATCAGACGTGGCG98400.12048576333363535No Hit
GGGCTACGCCTGTCTGAGCGTCGCT97070.1188572464105283No Hit
CGCCTGTCTGAGCGTCGCT96200.1177919759420297No Hit
ACCACAGGGTAGAACCACGGAC95830.11733892988071419No Hit
GGAATACCGGGTGCTGTAGGCTTA93030.1139104732004888No Hit
TTCAAGTAATCCAGGATAGGC91680.11225746730109441No Hit
TGCAAGTAATCCAGGATAGGCT90070.1102861047099648No Hit
GCCTGTCTGAGCGTCGCT86650.10609848976483235No Hit
TAGCAGCACATCATGGTTTACA86030.10533933149992532No Hit
GTCAGGATGGCCGAGCGGTCTAAGG84940.10400468229226614No Hit
TATTGCACTTGTCCCGGCCTGTTT84280.10319654607478443No Hit
TTCACAGTGGCTAAGTTCTGC81900.10028235789659284No Hit
CCCCCACTGCTAAATTTGACTGGCT81770.10012317955072525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)