FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6846990
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG79416111.59868789059134No Hit
CAACGGAATCCCAAAAGCAGCTG5820788.501224625711444No Hit
CCCCCACTGCTAAATTTGACTG2391213.4923521138485674No Hit
AACATTCAACGCTGTCGGTGAGT1929302.8177345081561387No Hit
TTCAAGTAATCCAGGATAGGCT1771112.5866986807341616No Hit
TAGCAGCACGTAAATATTGGCG1764872.577585187067602No Hit
AACATTCAACGCTGTCGGTGAG1595202.3297828680924026No Hit
AAGCTGCCAGTTGAAGAACTGT1547072.259489206205939No Hit
TATTGCACTTGTCCCGGCCTGTAT1511802.207977520048956No Hit
TATTGCACTTGTCCCGGCCTGTAA1362031.9892390670937157No Hit
TAGCTTATCAGACTGATGTTGACT932801.3623504634883357No Hit
TAGCTTATCAGACTGATGTTGAC912801.3331405478903868No Hit
TATTGCACTTGTCCCGGCCTGT690931.009100349204541No Hit
TGAGGTAGTAGGTTGTATAGTT617160.9013595755215065No Hit
TAGCTTATCAGACTGATGTTGA557610.8143870518286137No Hit
TATTGCACTTGTCCCGGCCTGTA554800.8102830586871019No Hit
TGTAAACATCCTTGACTGGAAGCT527470.7703677090225047No Hit
AGCAGCATTGTACAGGGCTATGA456300.6664242243672037No Hit
TTCAAGTAATTCAGGATAGGTT413840.6044115735527582No Hit
TGTAAACATCCCCGACTGGAAGCT406650.5939106088952956No Hit
TGAGGTAGTAGATTGTATAGTT382410.5585081911905815No Hit
CAACGGAATCCCAAAAGCAGCTGT355520.5192354596691393No Hit
CATAAAGTAGAAAGCACTACT355390.5190455952177526No Hit
CCCATAAAGTAGAAAGCACTAC258550.37761118389248416No Hit
ACTGGACTTGGAGTCAGAAGGC230120.3360892888699998No Hit
CTCCCACTGCTTCACTTGACTAG226100.3302180958348121No Hit
CATTGCACTTGTCTCGGTCTGA212410.3102239086080161No Hit
TTCTCACTACTGCACTTGACTAG211430.3087926227437166No Hit
CACCCGGCTGTGTGCACATGTGC207760.30343260323149296No Hit
CCCCCACTGCTAAATTTGACTGGCTT205810.30058463646069294No Hit
CCCATAAAGTAGAAAGCACT198320.2896455230692611No Hit
TATTGCACTTGTCCCGGCCTGTAG193200.2821677846761862No Hit
TGTAAACATCCTACACTCTCAGCT188030.2746170214941164No Hit
CACTAGATTGTGAGCTCCTGGAA187430.2737407240261779No Hit
TTAATGCTAATCGTGATAGGGGTT176410.2576460605317081No Hit
ACCCCCCACTGCTAAATTTGACTG176130.2572371217133368No Hit
TGATGTAGTAGGTTGTATAGTT173750.2537611417571809No Hit
CAACGGAATCCCAAAAGCAGCT168570.24619577361731215No Hit
AGCTCGGTCTGAGGCCCCTCAGT165880.242267039969388No Hit
TGAGAACTGAATTCCATGGGTT160480.2343803627579418No Hit
CACTAGATTGTGAGCTCCTGGA159760.23332880579641566No Hit
TATTGCACTTGTCCCGGCCTGTT156970.22925402257050176No Hit
CCCCCCACTGCTAAATTTGACTGGCT156280.22824628048237253No Hit
TATTGCACTTGTCCCGGCCTGTTT151330.22101682637188017No Hit
TGAGGTAGTAGTTTGTACAGTT148700.21717572247074995No Hit
TAGCTTATCAGACTGATGTTGACA148230.21648928945419812No Hit
CAAAGAATTCTCCTTTTGGGCT148220.21647468449639914No Hit
CTTCTCACTACTGCACTTGACTAG147320.21516023829449143No Hit
TCGTACGACTCTTAGCGG137990.20153381266804832No Hit
ACCGGGTGCTGTAGGCTTA135460.19783875834490774No Hit
TATTGCACTTGTCCCGGCCTGTAAT126820.18522007480659383No Hit
AACATTCAACGCTGTCGGTGAGTT125200.18285407164315998No Hit
CCCCCCACTGCTAAATTTGACTGG124600.18197777417522154No Hit
ATCCCGGACGAGCCCCCA122690.1791882272356174No Hit
AACGGAATCCCAAAAGCAGCTG121700.17774233641351894No Hit
TAGCAGCACGTAAATATTGGC116090.16954895508829426No Hit
TGAGAACTGAATTCCATAGGCTGT112090.16370697196870448No Hit
TATTGCACTTGTCCCGGCCTGTAAA111490.16283067450076602No Hit
TGTAAACATCCCCGACTGGAAGC110430.16128254897407474No Hit
ACCGGGTGCTGTAGGCTT110270.16104886964929116No Hit
GCCTGTCTGAGCGTCGCT109620.16009954739235782No Hit
TGATGTAGTAGATTGTATAGTT107660.15723697566375883No Hit
CAACACCAGTCGATGGGCTGT104940.15326442714243776No Hit
TTCAAGTAATCCAGGATAGGCTT98680.14412172356027977No Hit
TCGTTTCCCGGGCGGCGCACCA93630.1367462198717977No Hit
CTCCCACTGCTTCACTTGACTAGCCT92300.13480376048453407No Hit
CAAAGAATTCTCCTTTTGGGCTT90560.13226249782751254No Hit
TGTCAGTTTGTCAAATACCCCA87610.12795403527681506No Hit
AGCAGCATTGTACAGGGCTATGT86690.12661037915930942No Hit
TGAGGTAGTAGTTTGTGCTGTT85670.12512067346381403No Hit
CCCCCCACTGCTAAATTTGACTGT83940.12259401576459146No Hit
TTCAAGTAATCCAGGATAGGC82690.12076839603971964No Hit
GGAATACCGGGTGCTGTAGGCTTT79430.11600717979725397No Hit
CGCGACCTCAGATCAGACGTGGCG79390.11594875996605808No Hit
ACCGGGTGCTGTAGGCTTT78410.11451747410175857No Hit
TGTCAGTTTGTCAAATACCCCAA77290.11288171882827346No Hit
TGAGGTAGTAGGTTGTGTGGTT74660.10904061492714318No Hit
CGCGACCTCAGATCAGACGTGG72180.10541858539299752No Hit
GGAATACCGGGTGCTGTAGGCTT71350.10420637389568263No Hit
CGCCTGTCTGAGCGTCGCT70270.10262903845339338No Hit
TATTGCACTTGTCCCGGCCTGA69900.10208865501483133No Hit
CCCCCCACTGCTAAATTTGACTGA69760.1018841856056457No Hit
CAAAGTGCTGTTCGTGCAGGTAG69530.10154827157626929No Hit
AACCGGGCGGAAACACCA69320.10124156746249083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)