FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6778090
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5325527.856962654671154No Hit
CAACGGAATCCCAAAAGCAGCTG4892367.2179035687044575No Hit
TAGCAGCACGTAAATATTGGCG2596763.8311087636782633No Hit
AAGCTGCCAGTTGAAGAACTGT2365543.4899802156654753No Hit
AACATTCAACGCTGTCGGTGAGT2349173.465828869194714No Hit
TTCAAGTAATCCAGGATAGGCT1771052.6129042252315915No Hit
TAGCTTATCAGACTGATGTTGAC1661032.4505871122985976No Hit
CCCCCACTGCTAAATTTGACTG1642092.4226441372126954No Hit
TAGCTTATCAGACTGATGTTGACT1334011.968120812795345No Hit
AACATTCAACGCTGTCGGTGAG1137801.6786439837771407No Hit
TATTGCACTTGTCCCGGCCTGTAA1022571.508640339682713No Hit
TGTAAACATCCTTGACTGGAAGCT861871.2715529005958905No Hit
TATTGCACTTGTCCCGGCCTGTAT860371.2693398877854971No Hit
TAGCTTATCAGACTGATGTTGA792101.1686182980751214No Hit
AGCAGCATTGTACAGGGCTATGA599040.8837887959587435No Hit
TGTAAACATCCCCGACTGGAAGCT508760.7505949316105275No Hit
TGAGGTAGTAGGTTGTATAGTT485900.7168686163801308No Hit
TATTGCACTTGTCCCGGCCTGT451020.6654086918291141No Hit
CATAAAGTAGAAAGCACTACT419960.6195845732352329No Hit
CAACGGAATCCCAAAAGCAGCTGT396050.5843091490375607No Hit
TGTAAACATCCTACACTCTCAGCT353840.522034968553088No Hit
TATTGCACTTGTCCCGGCCTGTA351550.5186564356625539No Hit
CCCATAAAGTAGAAAGCACTAC338510.49941797763086654No Hit
ACTGGACTTGGAGTCAGAAGGC338510.49941797763086654No Hit
TTAATGCTAATCGTGATAGGGGTT308440.45505444749184504No Hit
TTCAAGTAATTCAGGATAGGTT302890.4468663000933891No Hit
TAGCTTATCAGACTGATGTTGACA264500.3902279255660518No Hit
TGAGGTAGTAGATTGTATAGTT255860.3774809717781853No Hit
CAAAGAATTCTCCTTTTGGGCT241160.35579344623632914No Hit
AACATTCAACGCTGTCGGTGAGTT223750.3301077442170287No Hit
CCCCCACTGCTAAATTTGACTGGCTT216600.31955904982081973No Hit
CAACGGAATCCCAAAAGCAGCT210660.31079551909166153No Hit
CAAAGAATTCTCCTTTTGGGCTT201040.29660273026767126No Hit
TTCTCACTACTGCACTTGACTAG183270.2703859051738764No Hit
CTTCTCACTACTGCACTTGACTAG179850.2653402359661793No Hit
CATTGCACTTGTCTCGGTCTGA174630.2576389513860099No Hit
CCCATAAAGTAGAAAGCACT157070.23173194808567016No Hit
TGTAAACATCCCCGACTGGAAGC149770.2209619524084218No Hit
CGCCTGTCTGAGCGTCGCT148040.21840961096710135No Hit
CGCGACCTCAGATCAGACGTGGCG145160.21416062637114583No Hit
CCCCCCACTGCTAAATTTGACTGGCT143650.21193286014201643No Hit
TTCAAGTAATCCAGGATAGGCTT131340.19377140167805385No Hit
CACCCGGCTGTGTGCACATGTGC128960.19026008801889618No Hit
ACCCCCCACTGCTAAATTTGACTG127140.18757496580895208No Hit
TAGCAGCACGTAAATATTGGC126510.1866455004285868No Hit
AGCTCGGTCTGAGGCCCCTCAGT120420.17766066841838926No Hit
CACTAGATTGTGAGCTCCTGGAA119830.17679021671296782No Hit
CCCCCCACTGCTAAATTTGACTGG117400.17320513596013037No Hit
TGTAAACATCCTACACTCAGCT115880.17096261631226495No Hit
TATTGCACTTGTCCCGGCCTGTAG114930.16956104153234908No Hit
CTACGCCTGTCTGAGCGTCGCT114320.16866108298945573No Hit
TGAGGTAGTAGTTTGTACAGTT112590.16610874154813524No Hit
AGCAGCATTGTACAGGGCTATGT110940.16367442745670238No Hit
ATCACATTGCCAGGGATTTCCA105720.15597314287653308No Hit
CACTAGATTGTGAGCTCCTGGA105180.1551764582647914No Hit
TGTCAGTTTGTCAAATACCCCA104680.15443878732799357No Hit
TGATGTAGTAGGTTGTATAGTT102280.150897966831364No Hit
TATTGCACTTGTCCCGGCCTGTAAT100910.14887674846453794No Hit
ACTGGACTTGGAGTCAGAAGGCA97730.14418516130650375No Hit
CTCCCACTGCTTCACTTGACTAG97110.14327044934487446No Hit
CGCGACCTCAGATCAGACGTGG96730.14270981943290809No Hit
AACGGAATCCCAAAAGCAGCTG96470.14232623054577323No Hit
TATTGCACTTGTCCCGGCCTGTT94950.14008371089790783No Hit
TAGCAGCACGTAAATATTGGCGT93040.13726580791934012No Hit
TATTGCACTTGTCCCGGCCTGTAAA92890.13704450663830076No Hit
TCGTACGACTCTTAGCGG92320.13620356177035123No Hit
GCCTGTCTGAGCGTCGCT92050.1358052194644804No Hit
CAGTGCAATAGTATTGTCAAAGC82900.12230584132108012No Hit
TACGCCTGTCTGAGCGTCGCT82430.12161243064049017No Hit
TCGTTTCCCGGCCAATGCACCA82270.12137637594071486No Hit
CCCGCCACTGCTAAATTTGACTG81900.12083049944748446No Hit
ACCACAGGGTAGAACCACGGAC80760.11914860971158543No Hit
TGTCAGTTTGTCAAATACCCCAA79670.11754048706936614No Hit
ATCACATTGCCAGGGATTTCC79390.11712739134475936No Hit
ACTTGACTTGGAGTCAGAAGGC78570.11591761100841091No Hit
AACATTCAACGCTGTCGGTGAGA78150.11529796742150075No Hit
TGAGGTAGTAGTTTGTGCTGTT77500.11433899520366356No Hit
ATGCACCTGGGCAAGGATTCTGA77440.11425047469124784No Hit
CAACGGAATCCCAAAAGCAGCTGA76620.1130406943548994No Hit
CTCCCACTGCTTCACTTGACTAGCCT76280.11253907811787686No Hit
TGTAAACATCCTTGACTGGAAGC74700.11020803795759573No Hit
TTATAAAGCAATGAGACTGATT73940.10908677813366303No Hit
CAAAGTGCTGTTCGTGCAGGTAG73760.1088212165964158No Hit
CAACACCAGTCGATGGGCTGT72120.10640165592371892No Hit
GGGCTACGCCTGTCTGAGCGTCGCT72040.10628362857383128No Hit
TATTGCACTTGTCCCGGCCTGTTT71860.10601806703658405No Hit
ACTGGACTTGGAGTCAGAAGGCT68660.10129697304107795No Hit
TTCAAGTAATCCAGGATAGGC68440.1009723978288869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)