FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7271388
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG80498411.070568645216017No Hit
CAACGGAATCCCAAAAGCAGCTG6288078.647688721878135No Hit
TAGCAGCACGTAAATATTGGCG2442593.3591798429680826No Hit
CCCCCACTGCTAAATTTGACTG2396213.2953955970992057No Hit
AACATTCAACGCTGTCGGTGAGT2330773.205399024230312No Hit
TTCAAGTAATCCAGGATAGGCT2153322.961360334505599No Hit
AAGCTGCCAGTTGAAGAACTGT1810492.489882261818514No Hit
AACATTCAACGCTGTCGGTGAG1635572.2493229628236038No Hit
TATTGCACTTGTCCCGGCCTGTAA1360081.870454444185897No Hit
TATTGCACTTGTCCCGGCCTGTAT1177041.6187280887775484No Hit
TAGCTTATCAGACTGATGTTGACT1090121.4991910760366522No Hit
TAGCTTATCAGACTGATGTTGAC1047931.44116914129737No Hit
TAGCTTATCAGACTGATGTTGA696070.9572725317367194No Hit
TGAGGTAGTAGGTTGTATAGTT634800.8730107649323624No Hit
TATTGCACTTGTCCCGGCCTGT564370.7761516783315647No Hit
TGTAAACATCCTTGACTGGAAGCT532670.7325561502150622No Hit
TATTGCACTTGTCCCGGCCTGTA481940.6627895526961289No Hit
TTCAAGTAATTCAGGATAGGTT444730.6116163791562216No Hit
TTAATGCTAATCGTGATAGGGGTT397290.5463743648392851No Hit
CAACGGAATCCCAAAAGCAGCTGT389910.5362249958329827No Hit
TGAGGTAGTAGATTGTATAGTT378470.5204920986199609No Hit
TGTAAACATCCCCGACTGGAAGCT376410.5176590769190147No Hit
AGCAGCATTGTACAGGGCTATGA350920.48260387150293726No Hit
CATAAAGTAGAAAGCACTACT317370.4364641248685946No Hit
TTCTCACTACTGCACTTGACTAG277930.38222413657475024No Hit
ACTGGACTTGGAGTCAGAAGGC244070.3356580614320127No Hit
CCCATAAAGTAGAAAGCACTAC226900.31204496307995117No Hit
CTTCTCACTACTGCACTTGACTAG226370.31131607885592133No Hit
CCCCCACTGCTAAATTTGACTGGCTT218500.30049283575570446No Hit
CAAAGAATTCTCCTTTTGGGCT213610.29376784734908934No Hit
CAACGGAATCCCAAAAGCAGCT199440.2742805087556874No Hit
CATTGCACTTGTCTCGGTCTGA197450.2715437547824432No Hit
CACCCGGCTGTGTGCACATGTGC196890.27077361296082675No Hit
ACCCCCCACTGCTAAATTTGACTG194530.26752801528401454No Hit
GGAATACCGGGTGCTGTAGGCTTT181470.24956720780131664No Hit
TGAGAACTGAATTCCATGGGTT177840.24457503849333856No Hit
TAGCTTATCAGACTGATGTTGACA177420.24399743212712618No Hit
CCCCCCACTGCTAAATTTGACTGG175140.24086185471054494No Hit
AACATTCAACGCTGTCGGTGAGTT167080.2297773134922796No Hit
TGTAAACATCCTACACTCTCAGCT164450.22616039743718805No Hit
CCCATAAAGTAGAAAGCACT164340.22600911957937056No Hit
TATTGCACTTGTCCCGGCCTGTAG159560.219435409030573No Hit
CTCCCACTGCTTCACTTGACTAG157460.2165473771995113No Hit
TGAGGTAGTAGTTTGTACAGTT149190.20517403279813978No Hit
GGAATACCGGGTGCTGTAGGCTT147110.20231350603213583No Hit
TAGCAGCACGTAAATATTGGC146650.20168088953580804No Hit
CAAAGAATTCTCCTTTTGGGCTT141230.19422701690516309No Hit
CCCCCCACTGCTAAATTTGACTGGCT136550.18779083168165417No Hit
CACTAGATTGTGAGCTCCTGGAA135890.18688316453474907No Hit
AGCTCGGTCTGAGGCCCCTCAGT130930.18006190840043193No Hit
CACTAGATTGTGAGCTCCTGGA125950.17321314720105707No Hit
AACGGAATCCCAAAAGCAGCTG123060.16923866530021503No Hit
TATTGCACTTGTCCCGGCCTGTT122490.16845477094606973No Hit
TATTGCACTTGTCCCGGCCTGTAAT122120.1679459272425017No Hit
TATTGCACTTGTCCCGGCCTGTAAA119290.16405396053683285No Hit
TTCAAGTAATCCAGGATAGGCTT111850.15382207633535716No Hit
TGTAAACATCCCCGACTGGAAGC110020.15130536288257482No Hit
ACCGGGTGCTGTAGGCTTA104650.14392025291457422No Hit
ACCGGGTGCTGTAGGCTT104080.1431363585604289No Hit
TGAGAACTGAATTCCATAGGCTGT102140.14046836724982906No Hit
TATTGCACTTGTCCCGGCCTGTTT100670.13844674496808587No Hit
TGATGTAGTAGGTTGTATAGTT100250.13786913860187355No Hit
CGCCCCACTGCTAAATTTGACTG98990.1361363195032365No Hit
GGGAATACCGGGTGCTGTAGGCTT98900.13601254671047674No Hit
CGCGACCTCAGATCAGACGTGGCG98670.13569623846231285No Hit
CAACACCAGTCGATGGGCTGT98440.13537993021414893No Hit
CAACGGAATCCCAAAAGCAGCTGA95660.1315567261711244No Hit
TTCAAGTAATCCAGGATAGGC90000.12377279275978671No Hit
TGAGGTAGTAGTTTGTGCTGTT89970.12373153516220012No Hit
GGGAATACCGGGTGCTGTAGGCTTT89760.12344273197909396No Hit
CTCCCACTGCTTCACTTGACTAGCCT85320.1173366075362778No Hit
TTTAAACATCCTTGACTGGAAGCT82640.11365092881854193No Hit
CGCGACCTCAGATCAGACGTGG81270.11176683186208741No Hit
TTCACAGTGGCTAAGTTCCG80410.11058411406460499No Hit
ACCGGGTGCTGTAGGCTTT79980.10999275516586379No Hit
ACTGGACTTGGAGTCAGAAGGCT79890.10986898237310401No Hit
TGAATACCGGGTGCTGTAGGCTTT77270.10626581885054133No Hit
CCCGCCACTGCTAAATTTGACTG76340.10498683332535687No Hit
ATGCACCTGGGCAAGGATTCTGA75950.10445048455673112No Hit
TGTCAGTTTGTCAAATACCCCA75620.10399665098327857No Hit
ACTGGACTTGGAGTCAGAAGGCA75380.10366659020258581No Hit
AACATTCAACGCTGTCGGTGA74560.10253888253521887No Hit
CCCCCCACTGCTAAATTTGACTGT72820.1001459418751963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)