FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6638491
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3764695.671002642016084No Hit
CCCCCCACTGCTAAATTTGACTG2725424.105481200471614No Hit
AACATTCAACGCTGTCGGTGAGT2636683.9718062433164407No Hit
TAGCAGCACGTAAATATTGGCG2571163.8731091147069416No Hit
TTCAAGTAATCCAGGATAGGCT2352113.543139547827963No Hit
AAGCTGCCAGTTGAAGAACTGT2195733.3075739652279412No Hit
AACATTCAACGCTGTCGGTGAG1218461.8354472424531416No Hit
GCCTGTCTGAGCGTCGCT1087571.6382789401989097No Hit
CCCCCACTGCTAAATTTGACTG1065341.604792414420687No Hit
CATAAAGTAGAAAGCACTACT931991.4039184507443032No Hit
TAGCTTATCAGACTGATGTTGAC853691.2859699591367977No Hit
CGCCTGTCTGAGCGTCGCT767961.1568291649412494No Hit
TAGCTTATCAGACTGATGTTGA652050.9822262318349154No Hit
TGAGGTAGTAGGTTGTATAGTT639280.9629899325012267No Hit
TAGCTTATCAGACTGATGTTGACT575780.8673356640839011No Hit
TATTGCACTTGTCCCGGCCTGT542210.8167669429694189No Hit
TATTGCACTTGTCCCGGCCTGTAA472400.7116075023676315No Hit
CCCATAAAGTAGAAAGCACTAC437510.6590503775632143No Hit
TATTGCACTTGTCCCGGCCTGTAT401890.6053936052636057No Hit
AGCAGCATTGTACAGGGCTATGA378310.569873484802495No Hit
TGTAAACATCCTTGACTGGAAGCT352070.5303464296328789No Hit
CTACGCCTGTCTGAGCGTCGCT343460.5173766146553486No Hit
TACGCCTGTCTGAGCGTCGCT265760.4003319429069046No Hit
TCCTGTCTGAGCGTCGCT263840.3974397193579083No Hit
TATTGCACTTGTCCCGGCCTGTA256470.38633779875577146No Hit
TGAGGTAGTAGATTGTATAGTT255410.3847410503380964No Hit
ACTGGACTTGGAGTCAGAAGGC253160.38135172586661636No Hit
ACGCCTGTCTGAGCGTCGCT236420.35613515179880484No Hit
ACCGGGTGCTGTAGGCTT230340.34697644389364996No Hit
CATTGCACTTGTCTCGGTCTGA228230.3437980107226175No Hit
CCCATAAAGTAGAAAGCACT224450.3381039456105311No Hit
TGTAAACATCCCCGACTGGAAGCT214770.32352231855100805No Hit
CAACGGAATCCCAAAAGCAGCTGT208230.31367068208723936No Hit
TTCAAGTAATTCAGGATAGGTT206390.31089896785278465No Hit
TCCACCACGTTCCCGTGG203680.30681671482269085No Hit
TAGCAGCACATCATGGTTTACA201880.30410525524550686No Hit
AACCGGGCGGAAACACCA194740.2933497989226768No Hit
TTCACAGTGGCTAAGTTCCG187800.28289561588620066No Hit
AACATTCAACGCTGTCGGTGAGTT186510.28095240318921877No Hit
GTCAGGATGGCCGAGCGGTCTAAGG185880.28000339233720434No Hit
TTCACAGTGGCTAAGTTCCGC183210.27598139396438137No Hit
TGTAAACATCCTACACTCTCAGCT179890.2709802574109086No Hit
CAACACCAGTCGATGGGCTGT169390.25516340987733505No Hit
TCAGGCTCAGTCCCCTCCCGAT167620.2524971412931041No Hit
GCGTTCGCGCTTTCCCCTG165980.25002670034500313No Hit
TCGTACGACTCTTAGCGG155600.23439061678324188No Hit
TTCTCACTACTGCACTTGACTAG141900.21375339666800786No Hit
CGCGACCTCAGATCAGACGTGG137350.2068994294034593No Hit
TGTCAGTTTGTCAAATACCCCAA128700.19386935976865827No Hit
TGAGGTAGTAGTTTGTACAGTT124970.18825061297816026No Hit
ACCGGGTGCTGTAGGCTTT110500.16645349071046417No Hit
TTCAAGTAATCCAGGATAGGC108590.16357633082578557No Hit
ATCCCACCGCTGCCACCA106690.16071423460542464No Hit
TGAGGTAGTAGGTTGTGTGGTT105410.15878608557276044No Hit
CCCCCACTGCTAAATTTGACTGGCTT105280.15859025793663048No Hit
GGAATACCGGGTGCTGTAGGCTT104680.15768643807756916No Hit
CTCCCACTGCTTCACTTGACTAG104180.15693325486168466No Hit
ATCACATTGCCAGGGATTTCC103660.15614994431716486No Hit
TGTAAACATCCTACACTCAGCT102910.15502016949333816No Hit
GGAATACCGGGTGCTGTAGGCTTT102530.154447750249266No Hit
CAACGGAATCCCAAAAGCAGCT101840.15340835741134543No Hit
TAGCAGCACGTAAATATTGGC99870.1504408155407607No Hit
ACCGGGTGCTGTAGGCTTA98980.14910014941648636No Hit
TAGCAGCACATAATGGTTTGT98560.14846747551514342No Hit
TAGCAGCACGTAAATATTGGCGT97500.14687072709746837No Hit
CACCCGGCTGTGTGCACATGTGC97030.146162734874537No Hit
CTTCTCACTACTGCACTTGACTAG95830.14435509515641431No Hit
ACCACAGGGTAGAACCACGGAC95680.144129140191649No Hit
CGCGACCTCAGATCAGACGTGGCG93040.14015233281177905No Hit
ATCCCGGACGAGCCCCCA91840.13834469309365638No Hit
TCAGGCTCAGTCCCCTCCCGA91580.13795303782139645No Hit
TTCACAGTGGCTAAGTTCTGC90550.1364014803966745No Hit
TGAGGTAGTAGTTTGTGCTGTT88660.13355444784063125No Hit
GGGCTACGCCTGTCTGAGCGTCGCT88580.13343393852608973No Hit
AACATTCAACGCTGTCGGTGA88150.13278620096042912No Hit
TGATGTAGTAGGTTGTATAGTT87240.1314154075075194No Hit
AGCTCGGTCTGAGGCCCCTCAGT87090.13118945254275408No Hit
AACGGAATCCCAAAAGCAGCTG86140.1297584044325736No Hit
TAGCTTATCAGACTGATGTTGACA85670.12905041220964222No Hit
TCGTTTCCCGGCCAATGCACCA85600.1289449665594184No Hit
CAAAGTGCTGTTCGTGCAGGT85030.12808633769331013No Hit
AACATTCAACGCTGTCGGTGAGA83160.12526943246590228No Hit
TGTCAGTTTGTCAAATACCCCA82990.12501335017250156No Hit
TTCAAGTAATCCAGGATAGGCTT81610.12293456449666046No Hit
TATTGCACTTGTCCCGGCCTGTT78460.11818951023658841No Hit
ACTGGACTTGGAGTCAGAAGG77970.11745139068502163No Hit
AGCAGCATTGTACAGGGCTATG77820.1172254357202563No Hit
TTATAAAGCAATGAGACTGATT75490.11371560193423474No Hit
CATAAAGTAGAAAGCACTAC73500.11071793273501464No Hit
AGCAGCATTGTACAGGGCTATGT73470.11067274174206157No Hit
CCCCCCACTGCTAAATTTGACTGG73050.11004006784071863No Hit
CCCCCCACTGCTAAATTTGACTGGCT72560.10930194828915187No Hit
TGTAAACATCCCCGACTGGAAGC70740.10656036138333244No Hit
TACCTGGTTGATCCTGCC69850.10521969525905811No Hit
CAAAGTGCTGTTCGTGCAGGTAG69650.10491842197270433No Hit
TGAGGTAGTAGGTTGTATAGT67920.10231240804574412No Hit
TCAGGCTCAGTCCCCTCCCGATA67710.10199607109507265No Hit
GTCAGGATGGCCGAGTGGTCTAAGG67350.10145377917963586No Hit
CCCATAAAGTAGAAAGCACTA66610.10033906802012686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)