FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8350694
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT7614049.117852959286976No Hit
CAACGGAATCCCAAAAGCAGCTG5060076.059460447239475No Hit
AAGCTGCCAGTTGAAGAACTGT3515464.209781845676539No Hit
TAGCAGCACGTAAATATTGGCG3150473.772704400376783No Hit
TTCAAGTAATCCAGGATAGGCT3016703.6125141215807934No Hit
TCCTGTCTGAGCGTCGCT2794403.346308702007282No Hit
AACATTCAACGCTGTCGGTGAGT2158462.5847672061747202No Hit
TAGCTTATCAGACTGATGTTGAC1923982.303976172519314No Hit
CCCCCCACTGCTAAATTTGACTG1750642.0964006105360826No Hit
TAGCTTATCAGACTGATGTTGA1337631.6018189625916124No Hit
TAGCTTATCAGACTGATGTTGACT1291861.5470091467846863No Hit
TGAGGTAGTAGGTTGTATAGTT1120401.3416848946925848No Hit
AACATTCAACGCTGTCGGTGAG1099541.3167049349431317No Hit
CATAAAGTAGAAAGCACTACT1015041.2155157403684054No Hit
TGTAAACATCCTTGACTGGAAGCT1013371.2135159065821355No Hit
CCCCCACTGCTAAATTTGACTG734970.8801304418530963No Hit
TGAGGTAGTAGATTGTATAGTT729400.873460337547993No Hit
TATTGCACTTGTCCCGGCCTGTAA690120.8264223308865108No Hit
TATTGCACTTGTCCCGGCCTGT673390.806388067865976No Hit
TTCAAGTAATTCAGGATAGGTT642450.7693372550832303No Hit
AGCAGCATTGTACAGGGCTATGA608830.7290771282003627No Hit
CGCCTGTCTGAGCGTCGCT604130.7234488534725377No Hit
TATTGCACTTGTCCCGGCCTGTAT600560.7191737596899132No Hit
GGAATACCGGGTGCTGTAGGCTTT485700.5816283053839597No Hit
CCCATAAAGTAGAAAGCACTAC450160.5390689683995127No Hit
TGTAAACATCCCCGACTGGAAGCT436890.5231780735828663No Hit
TGTCAGTTTGTCAAATACCCCAA375170.44926804885917265No Hit
GGAATACCGGGTGCTGTAGGCTT360100.4312216445722954No Hit
CAACGGAATCCCAAAAGCAGCTGT356830.4273058023680427No Hit
TATTGCACTTGTCCCGGCCTGTA335380.4016193145144583No Hit
TGAGGTAGTAGTTTGTACAGTT334990.4011522874625749No Hit
TGTAAACATCCTACACTCTCAGCT317240.37989656907557623No Hit
CATTGCACTTGTCTCGGTCTGA291660.3492643844930733No Hit
TGATGTAGTAGGTTGTATAGTT289040.3461269207086261No Hit
TGTCAGTTTGTCAAATACCCCA268910.3220211397998777No Hit
ACTGGACTTGGAGTCAGAAGGC243480.29156858100656063No Hit
AACCGGGCGGAAACACCA240020.28742521280267247No Hit
CAACACCAGTCGATGGGCTGT236770.2835333207036445No Hit
TTCTCACTACTGCACTTGACTAG233130.2791744015527332No Hit
CCCATAAAGTAGAAAGCACT225350.26985781062029096No Hit
TAGCTTATCAGACTGATGTTGACA223960.2681932783071682No Hit
TTCACAGTGGCTAAGTTCCG215920.2585653360068037No Hit
TGAGGTAGTAGTTTGTGCTGTT210250.2517754811755766No Hit
TGATGTAGTAGATTGTATAGTT190050.22758587489854135No Hit
AACATTCAACGCTGTCGGTGAGTT186530.22337065637897882No Hit
TGAATACCGGGTGCTGTAGGCTTT182960.21909556259635427No Hit
CTACGCCTGTCTGAGCGTCGCT179540.21500009460291564No Hit
CAAAGAATTCTCCTTTTGGGCT171020.20479734977715625No Hit
TAGCAGCACGTAAATATTGGC170110.20370761998942843No Hit
TGTAAACATCCTACACTCAGCT167420.2004863308366945No Hit
TTCACAGTGGCTAAGTTCCGC164730.19726504168396064No Hit
TACGCCTGTCTGAGCGTCGCT154800.18537381443985373No Hit
ACGCCTGTCTGAGCGTCGCT145970.17479984298311013No Hit
TTATAAAGCAATGAGACTGATT145430.1741531901420409No Hit
TTCAAGTAATCCAGGATAGGCTT143860.1722731068818951No Hit
TAGCAGCACGTAAATATTGGCGT143810.17221323161883312No Hit
TGAATACCGGGTGCTGTAGGCTT138290.1656030025767918No Hit
TTCAAGTAATCCAGGATAGGC137970.16521980089319524No Hit
TGTAAACATCCCCGACTGGAAGC135800.16262121447630581No Hit
CCCCCACTGCTAAATTTGACTGGCTT135650.16244158868711991No Hit
CCCTGTCTGAGCGTCGCT134430.16098063226840786No Hit
CAAAGAATTCTCCTTTTGGGCTT132510.1586814221668283No Hit
AGCTCGGTCTGAGGCCCCTCAGT129620.15522063196184652No Hit
TTCACAGTGGCTAAGTTCTGC125800.15064616186391214No Hit
CACCCGGCTGTGTGCACATGTGC119050.14256300135054642No Hit
CTTCTCACTACTGCACTTGACTAG118850.14232350029829854No Hit
CAACGGAATCCCAAAAGCAGCT117420.14061106777472626No Hit
AGCAGCATTGTACAGGGCTATGT117320.14049131724860234No Hit
TGAGGTAGTAGGTTGTATAGT113410.13580907167715642No Hit
TATTGCACTTGTCCCGGCCTGTT109320.13091127515868742No Hit
ACCGGGTGCTGTAGGCTT106220.12719900884884539No Hit
AGCAGCATTGTACAGGGCTATG101420.12145098359489642No Hit
CAAAGTGCTGTTCGTGCAGGT101040.12099593159562547No Hit
TGTCAGTTTGTCAAATACCCC100180.11996607707095962No Hit
AACGGAATCCCAAAAGCAGCTG99810.11952300012430105No Hit
TAGCAGCACATAATGGTTTGT99490.11913979844070445No Hit
ATCCCACCGCTGCCACCA98000.1173555156014578No Hit
CGCGACCTCAGATCAGACGTGGCG97700.116996264023086No Hit
GCGTTCGCGCTTTCCCCTG96960.11611011012976886No Hit
GCCTGTCTGAGCGTCGCA96850.11597838455103253No Hit
ACCACAGGGTAGAACCACGGAC96380.11541555707825003No Hit
CGCGACCTCAGATCAGACGTGG96050.11502038034204103No Hit
CAAAGTGCTGTTCGTGCAGGTAG94950.11370312455467772No Hit
AGAGGTAGTAGGTTGCATAGTT93900.11244574403037641No Hit
ATCCCGGACGAGCCCCCA91640.10973938213997543No Hit
TAGCAGCACATCATGGTTTACA88610.10611094119842016No Hit
TCGTACGACTCTTAGCGG88350.10579958983049792No Hit
TTCACAGTGGCTAAGTTCTG88250.10567983930437398No Hit
CCCCCCACTGCTAAATTTGACTGGCT87730.10505713656852951No Hit
TGAGGTAGTAGGTTGTGTGGTT87400.10466195983232052No Hit
TCGTTTCCCGGCCAATGCACCA85710.102638175940826No Hit
CTCCCACTGCTTCACTTGACTAG84730.10146462078481143No Hit
TGATGTAGTAGTTTGTACAGTT84600.10130894510085028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)