FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4956983
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG2470734.9843422904617585No Hit
TAGCAGCACGTAAATATTGGCG1872893.7782861066902993No Hit
TTCAAGTAATCCAGGATAGGCT1774183.5791528839215303No Hit
CCCCCCACTGCTAAATTTGACTG1541013.108765957034753No Hit
AAGCTGCCAGTTGAAGAACTGT1518093.062528154726373No Hit
TAGCTTATCAGACTGATGTTGAC1488503.0028345870865403No Hit
GGAATACCGGGTGCTGTAGGCTTT1276532.5752156099788923No Hit
TAGCTTATCAGACTGATGTTGACT1252722.5271823607222377No Hit
AACATTCAACGCTGTCGGTGAGT1153722.3274641046781883No Hit
GGAATACCGGGTGCTGTAGGCTT766091.5454763512402605No Hit
TGTAAACATCCTTGACTGGAAGCT762551.5383349105695945No Hit
TAGCTTATCAGACTGATGTTGA762521.5382743898859448No Hit
AACATTCAACGCTGTCGGTGAG559941.1295983867606565No Hit
TGAATACCGGGTGCTGTAGGCTTT553411.1164250512862361No Hit
CCCCCACTGCTAAATTTGACTG521751.0525555564745734No Hit
TATTGCACTTGTCCCGGCCTGTAA518721.0464429674259523No Hit
CATAAAGTAGAAAGCACTACT518411.045817587028239No Hit
TGAGGTAGTAGGTTGTATAGTT473110.9544313547171737No Hit
TATTGCACTTGTCCCGGCCTGTAT415450.8381106007424274No Hit
AGCAGCATTGTACAGGGCTATGA343260.6924776623200039No Hit
TGAATACCGGGTGCTGTAGGCTT332470.6707103897673242No Hit
TGTAAACATCCCCGACTGGAAGCT311780.6289712916102396No Hit
TGTAAACATCCTACACTCTCAGCT294260.5936272123588078No Hit
CCCATAAAGTAGAAAGCACTAC287430.57984867004789No Hit
TTCAAGTAATTCAGGATAGGTT282790.5704881376434012No Hit
TGAGGTAGTAGATTGTATAGTT279380.5636089532685506No Hit
GCCTGTCTGAGCGTCGCT251930.5082325277290642No Hit
CCCCCACTGCTAAATTTGACTGGCTT240830.48583987477867085No Hit
TAGCTTATCAGACTGATGTTGACA240410.4849925852075748No Hit
TATTGCACTTGTCCCGGCCTGT232750.4695396373156817No Hit
CAAAGAATTCTCCTTTTGGGCT225370.45465154913785255No Hit
CAACGGAATCCCAAAAGCAGCTGT206410.4164024770712347No Hit
GGAATACCGGGTGCTGTAGGCTTA188310.37988833126924176No Hit
TAGCAGCACATCATGGTTTACA185420.3740581720776529No Hit
TATTGCACTTGTCCCGGCCTGTA179440.36199438247014365No Hit
TTAATGCTAATCGTGATAGGGGTT173000.3490026090466722No Hit
ACTGGACTTGGAGTCAGAAGGC148280.2991335657193095No Hit
CAAAGAATTCTCCTTTTGGGCTT141370.2851936349186592No Hit
TTCACAGTGGCTAAGTTCCGC135000.27234307642370364No Hit
TTCTCACTACTGCACTTGACTAG128690.2596135592960476No Hit
TCGTTTCCCGGCCAATGCACCA126260.2547113839204209No Hit
ATCACATTGCCAGGGATTTCCA123270.24867948911666632No Hit
ATCACATTGCCAGGGATTTCC123120.24837688569841776No Hit
CAACGGAATCCCAAAAGCAGCT121950.24601657903607901No Hit
TGTCAGTTTGTCAAATACCCCA118350.2387540969981136No Hit
GGAATACCGGGTGCTGTAGGCT116150.23431591353046802No Hit
TAGCAGCACGTAAATATTGGC115370.2327423757555755No Hit
TGAGGTAGTAGTTTGTACAGTT114040.23005929211377163No Hit
AACATTCAACGCTGTCGGTGAGTT110080.22207056187200963No Hit
CCCCCCACTGCTAAATTTGACTGGCT110020.22194952050471023No Hit
CATTGCACTTGTCTCGGTCTGA106560.21496946832377678No Hit
TGTCAGTTTGTCAAATACCCCAA103150.2080902839489262No Hit
TCCTGTCTGAGCGTCGCT101780.20532650606225603No Hit
TGATGTAGTAGGTTGTATAGTT100260.20226012475733726No Hit
TGTAAACATCCTACACTCAGCT98350.19840697456497228No Hit
GTCAGGATGGCCGAGCGGTCTAAGG97080.1958449322904678No Hit
TTCACAGTGGCTAAGTTCCG95970.19360566699542847No Hit
TTCAAGTAATCCAGGATAGGCTT92820.18725099521220873No Hit
CTTCTCACTACTGCACTTGACTAG91020.18361975419322601No Hit
TAGCAGCACGTAAATATTGGCGT87170.17585293312484632No Hit
CCCATAAAGTAGAAAGCACT86810.17512668492104974No Hit
CGCCTGTCTGAGCGTCGCT85720.17292776674844357No Hit
TGAATACCGGGTGCTGTAGGCTTA82350.16612927661845925No Hit
TGTAAACATCCCCGACTGGAAGC77650.15664770284667104No Hit
TGAGGTAGTAGTTTGTGCTGTT75310.15192708952199352No Hit
CTACGCCTGTCTGAGCGTCGCT74410.15011146901250216No Hit
TGTAGTGTTTCCTACTTTATGGA74190.14966765066573762No Hit
CGCGACCTCAGATCAGACGTGGCG71580.14440235118821268No Hit
CCCCCCACTGCTAAATTTGACTGG70960.1431515903927853No Hit
ATCACATTGCCAGGGATTTCCAA70040.1412956227608608No Hit
AGCTCGGTCTGAGGCCCCTCAGT69760.14073076304679683No Hit
TTCACAGTGGCTAAGTTCTGC67520.13621188533428497No Hit
AGCAGCATTGTACAGGGCTATGT67460.13609084396698556No Hit
TTCAAGTAATCCAGGATAGGC65920.13298411553963368No Hit
TTATAAAGCAATGAGACTGATT64980.13108780078527604No Hit
AACCGGGCGGAAACACCA64100.12931252739821783No Hit
TTTAAACATCCTTGACTGGAAGCT62410.1259031955526174No Hit
CAGTGCAATAGTATTGTCAAAGC61780.12463226119597344No Hit
CAACACCAGTCGATGGGCTGT61590.12424896353285861No Hit
TGCAAGTAATCCAGGATAGGCT61320.12370427738001119No Hit
TGTCAGTTTGTCAAATACCCC60490.12202987179903582No Hit
TGATGTAGTAGATTGTATAGTT58550.11811620092302111No Hit
TGTAAACATCCTTGACTGGAAGC58270.11755134120895715No Hit
ACCCCCCACTGCTAAATTTGACTG57290.11557433220973322No Hit
GTAATACCGGGTGCTGTAGGCTTT57080.11515068742418523No Hit
TAGCAGCACATAATGGTTTGT55190.11133788435425339No Hit
TACGCCTGTCTGAGCGTCGCT54890.11073267751775626No Hit
TAGCTTATCAGACTGATGTTGACC54770.11049059478315741No Hit
TATTGCACTTGTCCCGGCCTGTAG54720.11038972697707457No Hit
TATTGCACTTGTCCCGGCCTGTAAT52300.10550772516266446No Hit
CACCCGGCTGTGTGCACATGTGC51860.10462008846913536No Hit
TATTGCACTTGTCCCGGCCTGTT51660.10421661724480395No Hit
TGAATACCGGGTGCTGTAGGCT50060.10098884745015264No Hit
CACTAGATTGTGAGCTCCTGGAA49760.10038364061365553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)