FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7221407
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5270527.298466905410539No Hit
AAGCTGCCAGTTGAAGAACTGT2811533.893327159097943No Hit
CCCCCCACTGCTAAATTTGACTG2794943.870353796704714No Hit
TAGCAGCACGTAAATATTGGCG2548563.5291737468889375No Hit
GCCTGTCTGAGCGTCGCT2349153.2530364235113742No Hit
TTCAAGTAATCCAGGATAGGCT2299063.1836732093898044No Hit
AACATTCAACGCTGTCGGTGAGT1973542.7329023277596733No Hit
TAGCTTATCAGACTGATGTTGAC1692512.343739938768165No Hit
TAGCTTATCAGACTGATGTTGACT1242441.7204957427271443No Hit
AACATTCAACGCTGTCGGTGAG1036801.435731291699803No Hit
TAGCTTATCAGACTGATGTTGA1031191.4279627224999227No Hit
CCCCCACTGCTAAATTTGACTG980811.3581979245872722No Hit
TCCTGTCTGAGCGTCGCT882261.2217286742043483No Hit
TATTGCACTTGTCCCGGCCTGTAA865441.198436814321641No Hit
TGTAAACATCCTTGACTGGAAGCT794081.099619506281809No Hit
TGAGGTAGTAGGTTGTATAGTT737091.020701367475895No Hit
TATTGCACTTGTCCCGGCCTGTAT735411.0183749510310112No Hit
CATAAAGTAGAAAGCACTACT677350.9379751065131767No Hit
TATTGCACTTGTCCCGGCCTGT552110.7645462996338526No Hit
AGCAGCATTGTACAGGGCTATGA526290.7287914945106957No Hit
TTCAAGTAATTCAGGATAGGTT485160.6718358347618407No Hit
TGAGGTAGTAGATTGTATAGTT470570.6516320157553784No Hit
CCCATAAAGTAGAAAGCACTAC439910.6091749156362465No Hit
TTAATGCTAATCGTGATAGGGGTT415640.5755665066378338No Hit
CAACGGAATCCCAAAAGCAGCTGT387500.5365990311860279No Hit
TGTAAACATCCCCGACTGGAAGCT378960.5247730809245346No Hit
TATTGCACTTGTCCCGGCCTGTA340610.47166708648328504No Hit
TGTAAACATCCTACACTCTCAGCT296240.4102247664478681No Hit
CGCCTGTCTGAGCGTCGCT293520.40645818744186557No Hit
TAGCTTATCAGACTGATGTTGACA254940.3530336955111379No Hit
CCCCCACTGCTAAATTTGACTGGCTT245600.3400999278949379No Hit
TTCTCACTACTGCACTTGACTAG222840.30858252415353404No Hit
ACTGGACTTGGAGTCAGAAGGC219360.3037635186605602No Hit
TGTCAGTTTGTCAAATACCCCAA218920.3031542191154715No Hit
CAAAGAATTCTCCTTTTGGGCT216770.30017695997469745No Hit
TGAGGTAGTAGTTTGTACAGTT214170.2965765535719009No Hit
CATTGCACTTGTCTCGGTCTGA197830.2739493841020178No Hit
CCCATAAAGTAGAAAGCACT189290.26212343384052444No Hit
AACATTCAACGCTGTCGGTGAGTT178700.24745870160759528No Hit
TGTCAGTTTGTCAAATACCCCA172110.23833305614819936No Hit
CAAAGAATTCTCCTTTTGGGCTT153060.21195315538924758No Hit
TGAGGTAGTAGTTTGTGCTGTT144610.20025183458015872No Hit
TAGCAGCACGTAAATATTGGC144270.19978101220440836No Hit
CTACGCCTGTCTGAGCGTCGCT143000.1980223521538116No Hit
TTCACAGTGGCTAAGTTCCG138850.1922755496262709No Hit
CAACACCAGTCGATGGGCTGT137150.18992143774751927No Hit
CTTCTCACTACTGCACTTGACTAG136120.1884951229033345No Hit
CCCCCCACTGCTAAATTTGACTGGCT132120.1829560361298013No Hit
TGATGTAGTAGGTTGTATAGTT128010.17726462446999594No Hit
TTCAAGTAATCCAGGATAGGCTT125520.17381654295347154No Hit
TTTAAACATCCTTGACTGGAAGCT125280.17348419774705953No Hit
AGCTCGGTCTGAGGCCCCTCAGT123620.17118547673604326No Hit
GGAATACCGGGTGCTGTAGGCTTT122080.169052928328233No Hit
CAACGGAATCCCAAAAGCAGCT117880.16323688721602314No Hit
TACGCCTGTCTGAGCGTCGCT117210.16230909018145634No Hit
TAGCAGCACGTAAATATTGGCGT117200.1622952424645225No Hit
TGTAAACATCCTACACTCAGCT115660.1601626940567122No Hit
CACCCGGCTGTGTGCACATGTGC115040.15930413560681458No Hit
TGTAAACATCCCCGACTGGAAGC113930.1577670390271591No Hit
AACCGGGCGGAAACACCA111980.15506673422506168No Hit
CCCCCCACTGCTAAATTTGACTGG110440.1529341858172514No Hit
TTCACAGTGGCTAAGTTCCGC103670.14355928145304647No Hit
TATTGCACTTGTCCCGGCCTGTT101680.14080358578321372No Hit
ACCGGGTGCTGTAGGCTT100730.13948805267449957No Hit
TTCAAGTAATCCAGGATAGGC100730.13948805267449957No Hit
ACGCCTGTCTGAGCGTCGCT98190.135970732573306No Hit
ATCACATTGCCAGGGATTTCCA97110.13447517914445206No Hit
AGCAGCATTGTACAGGGCTATGT94630.13104094534486146No Hit
AACGGAATCCCAAAAGCAGCTG94060.13025162547963298No Hit
TGAGAACTGAATTCCATGGGTT94020.13019623461189767No Hit
TTATAAAGCAATGAGACTGATT93160.12900533095558803No Hit
ACCCCCCACTGCTAAATTTGACTG92980.12875607205077902No Hit
TATTGCACTTGTCCCGGCCTGTAG90450.12525259966651928No Hit
CAAAGTGCTGTTCGTGCAGGTAG88260.12221994965800986No Hit
GGAATACCGGGTGCTGTAGGCTT86880.12030896472114092No Hit
TTCACAGTGGCTAAGTTCTGC86220.11939501540350793No Hit
CTCCCACTGCTTCACTTGACTAG85430.11830104576573512No Hit
CCCTGTCTGAGCGTCGCT84860.11751172590050665No Hit
TAGCAGCACATCATGGTTTACA84660.11723477156182999No Hit
TGATGTAGTAGATTGTATAGTT82600.11438214187346041No Hit
CGCGACCTCAGATCAGACGTGGCG81380.11269272040753275No Hit
ATCACATTGCCAGGGATTTCC81000.11216650716404711No Hit
TCGTTTCCCGGCCAATGCACCA79050.10946620236194969No Hit
CACTAGATTGTGAGCTCCTGGAA77920.10790141034842655No Hit
ATCCCGGACGAGCCCCCA77780.1077075423113529No Hit
ACCACAGGGTAGAACCACGGAC77050.10669665897518309No Hit
TGAGGTAGTAGGTTGTGTGGTT76450.10586579595915312No Hit
CACTAGATTGTGAGCTCCTGGA73700.10205767380234905No Hit
AGCAGCATTGTACAGGGCTATG73160.10130989708792205No Hit
TATTGCACTTGTCCCGGCCTGTAAT72600.1005344249396274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)