FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8523055
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6210317.286483543752798No Hit
CCCCCCACTGCTAAATTTGACTG5222186.127122258392091No Hit
CCCCCACTGCTAAATTTGACTG2500872.9342413019744678No Hit
AACATTCAACGCTGTCGGTGAGT2384362.797541491871166No Hit
TTCAAGTAATCCAGGATAGGCT2252272.6425618513549427No Hit
TAGCTTATCAGACTGATGTTGAC2042692.3966641069428745No Hit
AACATTCAACGCTGTCGGTGAG2023222.3738201853678054No Hit
TAGCTTATCAGACTGATGTTGACT1943882.280731498271453No Hit
TAGCAGCACGTAAATATTGGCG1941842.278337990309813No Hit
TAGCTTATCAGACTGATGTTGA1716932.014453737538946No Hit
AAGCTGCCAGTTGAAGAACTGT1701401.9962325715368494No Hit
GCCTGTCTGAGCGTCGCT1521481.7851345556258877No Hit
TATTGCACTTGTCCCGGCCTGTAA1377511.6162162510977578No Hit
TGAGGTAGTAGGTTGTATAGTT1282191.5043784183018882No Hit
TATTGCACTTGTCCCGGCCTGTAT1114001.3070430731703597No Hit
TGAGGTAGTAGATTGTATAGTT812770.9536134637169418No Hit
TCCTGTCTGAGCGTCGCT774640.9088759840221611No Hit
CATAAAGTAGAAAGCACTACT730260.8568054529743149No Hit
TATTGCACTTGTCCCGGCCTGT716450.8406023427045819No Hit
TATTGCACTTGTCCCGGCCTGTA625410.7337861834752915No Hit
TGTAAACATCCTTGACTGGAAGCT559910.656935805295167No Hit
TTCAAGTAATTCAGGATAGGTT550590.646000759117476No Hit
TCGTACGACTCTTAGCGG517090.6066956038650461No Hit
TTAATGCTAATCGTGATAGGGGTT497740.5839924768759558No Hit
AGCAGCATTGTACAGGGCTATGA497240.5834058327677106No Hit
CAACACCAGTCGATGGGCTGT438570.5145690131062159No Hit
CATTGCACTTGTCTCGGTCTGA415070.4869967400186905No Hit
TGTAAACATCCCCGACTGGAAGCT378390.44396052823782084No Hit
TGATGTAGTAGGTTGTATAGTT343160.4026255843708623No Hit
CAACGGAATCCCAAAAGCAGCT333220.39096309949894725No Hit
CAACGGAATCCCAAAAGCAGCTGT296350.34770396295694445No Hit
GGAATACCGGGTGCTGTAGGCTTT286050.3356190943270928No Hit
TAGCTTATCAGACTGATGTTGACA278320.32654957641362164No Hit
CCCATAAAGTAGAAAGCACT260680.3058527722747301No Hit
TGAGGTAGTAGTTTGTACAGTT251600.2951993152689969No Hit
CCCATAAAGTAGAAAGCACTAC251340.2948942603327094No Hit
ACTGGACTTGGAGTCAGAAGGC248520.2915855875622063No Hit
CACCCGGCTGTGTGCACATGTGC238300.2795945819896739No Hit
TGATGTAGTAGATTGTATAGTT216950.25454487856760283No Hit
ACCGGGTGCTGTAGGCTT215990.253418521879772No Hit
TCGTTTCCCGGCCAATGCACCA213170.2501098491092689No Hit
TGAGAACTGAATTCCATGGGTT206780.24261253740589495No Hit
CAAAGAATTCTCCTTTTGGGCT197850.23213507363263525No Hit
GGAATACCGGGTGCTGTAGGCTT195160.22897892833027592No Hit
CCCCCACTGCTAAATTTGACTGGCTT182780.21445362021012418No Hit
TGAGGTAGTAGTTTGTGCTGTT182380.21398430492352802No Hit
TTCTCACTACTGCACTTGACTAG180880.21222437259879232No Hit
AACATTCAACGCTGTCGGTGAGTT170810.20040936025873352No Hit
TTATAAAGCAATGAGACTGATT166740.1956340772176174No Hit
TAGCAGCACGTAAATATTGGC162870.19109345181979936No Hit
TTCACAGTGGCTAAGTTCTG162870.19109345181979936No Hit
CACTAGATTGTGAGCTCCTGGA159870.18757358717032802No Hit
TGAATACCGGGTGCTGTAGGCTTT157860.18521527785518221No Hit
TATTGCACTTGTCCCGGCCTGTAG157610.18492195580105958No Hit
ACCGGGTGCTGTAGGCTTT157210.18445264051446342No Hit
TGTAAACATCCTACACTCTCAGCT145230.17039664768090784No Hit
TATTGCACTTGTCCCGGCCTGTT144130.16910603064276836No Hit
CACTAGATTGTGAGCTCCTGGAA142020.1666303925059735No Hit
ACCGGGTGCTGTAGGCTTA138670.16269987698073052No Hit
TTCACAGTGGCTAAGTTCCG125510.1472594040517162No Hit
AACGGAATCCCAAAAGCAGCTG122120.14328195699781357No Hit
ACCCCCCACTGCTAAATTTGACTG120010.14080631886101874No Hit
CTTCTCACTACTGCACTTGACTAG119590.14031353781009273No Hit
TTATGTAGTAGGTTGTATAGTT119000.13962129776236337No Hit
TTAGGTAGTAGGTTGTATAGTT117960.1384010780172133No Hit
CAAAGAATTCTCCTTTTGGGCTT114730.13461135707794916No Hit
CCCATAAAGTAGAAAGCACTA113190.13280449322455387No Hit
TGAATACCGGGTGCTGTAGGCTT111680.13103282801765329No Hit
ATCCCGGACGAGCCCCCA110340.12946062180755608No Hit
TGAGGTAGTAGGTTGTATAGT109310.12825213494457094No Hit
TATTGCACTTGTCCCGGCCTGTAAT108190.12693805214210163No Hit
TTCAAGTAATCCAGGATAGGCTT107980.12669166161663864No Hit
CGCCTGTCTGAGCGTCGCT106410.12484959911674863No Hit
TGAGGTAGTAAGTTGTATTGTT106330.12475573605942938No Hit
AGCAGCATTGTACAGGGCTATGT105290.12353551631427934No Hit
TATTGCACTTGTCCCGGCCTGTAAA101980.11965193231769594No Hit
TTTAAACATCCTTGACTGGAAGCT97490.11438386822565383No Hit
CGCGACCTCAGATCAGACGTGG96660.11341003900596676No Hit
CCCCCCACTGCTAAATTTGACTGT95760.11235407961112534No Hit
TGAGAACTGAATTCCATAGGCTGT95630.11220155214298158No Hit
TTCAAGTAATCCAGGATAGGC91040.10681615922929044No Hit
CACCCGTAGAACCGACCTTGCG90050.10565460389496488No Hit
AGCAGCATTGTACAGGGCTATG88070.1033314932263138No Hit
TTCACAGTGGCTAAGTTCTGC87870.10309683558301572No Hit
CAACGGAATCCCAAAAGCAGCTGA87150.1022520680671426No Hit
CCCCCCACTGCTAAATTTGACTGG87140.10224033518497769No Hit
ACTTGACTTGGAGTCAGAAGGC87120.10221686942064787No Hit
CCCCCCACTGCTAAATTTGACTGTA86030.10093798526467329No Hit
TTCACAGTGGCTAAGTTCCGC85780.10064466321055068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)