FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8088684
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4562035.640015112470706No Hit
TAGCAGCACGTAAATATTGGCG3510024.33942035564747No Hit
AACATTCAACGCTGTCGGTGAGT2944893.640752933357268No Hit
TTCAAGTAATCCAGGATAGGCT2868663.5465101616035435No Hit
CCCCCCACTGCTAAATTTGACTG2775693.4315718106925677No Hit
AAGCTGCCAGTTGAAGAACTGT2709043.3491727455294336No Hit
TAGCTTATCAGACTGATGTTGAC2403962.9720038513063436No Hit
TAGCTTATCAGACTGATGTTGA1941422.4001679383197563No Hit
TAGCTTATCAGACTGATGTTGACT1880522.3248775697010786No Hit
AACATTCAACGCTGTCGGTGAG1495731.8491635969460547No Hit
CATAAAGTAGAAAGCACTACT1259691.55734851305849No Hit
CCCCCACTGCTAAATTTGACTG1119981.3846257314539672No Hit
TGTAAACATCCTTGACTGGAAGCT927191.146280408531227No Hit
TATTGCACTTGTCCCGGCCTGTAA875261.0820796065219014No Hit
TGAGGTAGTAGGTTGTATAGTT784620.970021823080244No Hit
TATTGCACTTGTCCCGGCCTGTAT701910.8677678593946803No Hit
TATTGCACTTGTCCCGGCCTGT659900.8158311042933559No Hit
AGCAGCATTGTACAGGGCTATGA582640.7203149486368858No Hit
TTCAAGTAATTCAGGATAGGTT572670.707989086976324No Hit
CCCATAAAGTAGAAAGCACTAC460090.5688069901111231No Hit
TGAGGTAGTAGATTGTATAGTT458940.5673852508022319No Hit
CGCCTGTCTGAGCGTCGCT423840.5239912945047674No Hit
TATTGCACTTGTCCCGGCCTGTA418370.5172287605746497No Hit
TGTAAACATCCCCGACTGGAAGCT417410.5160419173254883No Hit
TGTAAACATCCTACACTCTCAGCT339950.42027850265877614No Hit
CATTGCACTTGTCTCGGTCTGA320150.3957998606448218No Hit
CCCATAAAGTAGAAAGCACT303570.3753020886957631No Hit
TGATGTAGTAGGTTGTATAGTT292620.36176465788501566No Hit
CAACGGAATCCCAAAAGCAGCTGT289190.35752416585936597No Hit
AACATTCAACGCTGTCGGTGAGTT287760.35575626393613596No Hit
TAGCTTATCAGACTGATGTTGACA285040.35239354139684526No Hit
TTCACAGTGGCTAAGTTCCG281830.34842503428246174No Hit
ACTGGACTTGGAGTCAGAAGGC275890.3410814416782755No Hit
CCCCCACTGCTAAATTTGACTGGCTT252080.3116452565089698No Hit
TTCTCACTACTGCACTTGACTAG252020.3115710788058972No Hit
CAACACCAGTCGATGGGCTGT246270.3044623822614408No Hit
TGTCAGTTTGTCAAATACCCCAA230880.28543580142332176No Hit
CAAAGAATTCTCCTTTTGGGCT217890.2693763287081063No Hit
TCGTACGACTCTTAGCGG194810.2408426389261838No Hit
TGAGGTAGTAGTTTGTACAGTT184520.2281211628492348No Hit
TTATAAAGCAATGAGACTGATT182540.2256732986478394No Hit
TGATGTAGTAGATTGTATAGTT172510.21327325928420493No Hit
CTTCTCACTACTGCACTTGACTAG163790.2024927664376554No Hit
TGTCAGTTTGTCAAATACCCCA162480.20087321992057053No Hit
CTACGCCTGTCTGAGCGTCGCT161620.19981000617653008No Hit
TAGCAGCACGTAAATATTGGC159260.19689234985567494No Hit
CAAAGAATTCTCCTTTTGGGCTT155770.19257768012695264No Hit
TTCAAGTAATCCAGGATAGGCTT154310.19077268935218636No Hit
CCCCCCACTGCTAAATTTGACTGGCT147720.18262550496471366No Hit
TGTAAACATCCTACACTCAGCT146470.1810801361507014No Hit
TGAGGTAGTAGTTTGTGCTGTT143580.17750724345270502No Hit
TGCAAGTAATCCAGGATAGGCT143350.17722289559092677No Hit
TAGCAGCACGTAAATATTGGCGT142910.17667892576839447No Hit
CAACGGAATCCCAAAAGCAGCT142890.17665419986737027No Hit
TTCAAGTAATCCAGGATAGGC141860.17538081596462418No Hit
TACGCCTGTCTGAGCGTCGCT138050.17067053181951478No Hit
AGCAGCATTGTACAGGGCTATGT132700.16405635329554227No Hit
TGTAAACATCCCCGACTGGAAGC127780.15797378164359No Hit
TTCACAGTGGCTAAGTTCTG127430.15754107837566655No Hit
ACCGGGTGCTGTAGGCTT126920.15691056789954955No Hit
TATTGCACTTGTCCCGGCCTGTT125130.15469759975788397No Hit
ACGCCTGTCTGAGCGTCGCT123490.15267007587389989No Hit
CCCATAAAGTAGAAAGCACTA122900.1519406617936861No Hit
CACTAGATTGTGAGCTCCTGGA118190.14611771210248786No Hit
CACCCGGCTGTGTGCACATGTGC117010.14465888394206028No Hit
ACTTGACTTGGAGTCAGAAGGC113960.14088818403587036No Hit
TTTAAACATCCTTGACTGGAAGCT112110.1386010381911322No Hit
AGCAGCATTGTACAGGGCTATG110980.1372040247832651No Hit
TTCACAGTGGCTAAGTTCCGC105300.13018186889239336No Hit
AACATTCAACGCTGTCGGTGA104640.12936591415859489No Hit
CACTAGATTGTGAGCTCCTGGAA102600.12684387225412688No Hit
TAGCAGCACATAATGGTTTGT97650.1207242117506383No Hit
ACCACAGGGTAGAACCACGGAC97080.1200195235714487No Hit
CAGTGCAATAGTATTGTCAAAGC97040.11997007176940032No Hit
CAAAGTGCTGTTCGTGCAGGT97040.11997007176940032No Hit
TCGTTTCCCGGCCAATGCACCA96520.11932719834277122No Hit
CAAAGTGCTGTTCGTGCAGGTAG93200.11522269877275464No Hit
TTCACAGTGGCTAAGTTCTGC92950.11491362500995218No Hit
TATTGCACTTGTCCCGGCCTGTAG92560.11443146993998035No Hit
ATCCCGGACGAGCCCCCA92390.11422129978127468No Hit
GTCAGGATGGCCGAGCGGTCTAAGG92280.1140853073256416No Hit
CTCCCACTGCTTCACTTGACTAG90120.1114149100150284No Hit
ACTGGACTTGGAGTCAGAAGG87940.10871978680339102No Hit
CCCCCCACTGCTAAATTTGACTGG85550.10576504163099956No Hit
ATCACATTGCCAGGGATTTCCA83970.10381169545008805No Hit
TAGCAGCACATCATGGTTTACA82990.10260012629990244No Hit
TGTCAGTTTGTCAAATACCCC81960.10132674239715632No Hit
AACATTCAACGCTGTCGGTGAGA81520.100782772574624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)