FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8048157
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6539858.125897643398359No Hit
CAACGGAATCCCAAAAGCAGCTG5612416.973534437760098No Hit
AAGCTGCCAGTTGAAGAACTGT3245634.032761786331951No Hit
TAGCAGCACGTAAATATTGGCG2839473.528099663065718No Hit
TTCAAGTAATCCAGGATAGGCT2663523.309478182396293No Hit
CCCCCACTGCTAAATTTGACTG2099852.609106656343806No Hit
AACATTCAACGCTGTCGGTGAGT2027652.519396676779541No Hit
TAGCTTATCAGACTGATGTTGAC1797922.233952444019171No Hit
TAGCTTATCAGACTGATGTTGACT1290051.6029135614526406No Hit
TAGCTTATCAGACTGATGTTGA1123621.3961208758725756No Hit
TGAGGTAGTAGGTTGTATAGTT1105181.373208797989403No Hit
AACATTCAACGCTGTCGGTGAG969421.2045242159167622No Hit
TGTAAACATCCTTGACTGGAAGCT923891.1479522578896013No Hit
TATTGCACTTGTCCCGGCCTGTAA773460.9610399001908139No Hit
TATTGCACTTGTCCCGGCCTGTAT666480.8281150578946211No Hit
TGAGGTAGTAGATTGTATAGTT655980.8150685927225325No Hit
GGAATACCGGGTGCTGTAGGCTTT643930.8000962207869454No Hit
GCCTGTCTGAGCGTCGCT626990.7790479236426426No Hit
TATTGCACTTGTCCCGGCCTGT605240.7520231029290309No Hit
CATAAAGTAGAAAGCACTACT604420.7510042361251154No Hit
AGCAGCATTGTACAGGGCTATGA598960.7442200742356293No Hit
TTCAAGTAATTCAGGATAGGTT501590.623235853873129No Hit
TGTAAACATCCCCGACTGGAAGCT498490.6193840403461314No Hit
GGAATACCGGGTGCTGTAGGCTT466680.5798594634771663No Hit
ACTGGACTTGGAGTCAGAAGGC455660.5661668876489363No Hit
TTCTCACTACTGCACTTGACTAG411650.5114835607704968No Hit
CAACGGAATCCCAAAAGCAGCTGT407600.5064513527755485No Hit
CCCATAAAGTAGAAAGCACTAC407240.5060040453982197No Hit
CCCCCACTGCTAAATTTGACTGGCTT382780.47561199414971655No Hit
TATTGCACTTGTCCCGGCCTGTA334030.41503912013644867No Hit
CGCCTGTCTGAGCGTCGCT328120.40769582402530163No Hit
CTTCTCACTACTGCACTTGACTAG326940.40622964984405746No Hit
CCCCCCACTGCTAAATTTGACTGGCT316550.3933198619261528No Hit
TGTAAACATCCTACACTCTCAGCT296260.3681091211317075No Hit
TGAGGTAGTAGTTTGTACAGTT291570.362281700021508No Hit
CCCCCCACTGCTAAATTTGACTGG271380.3371952112763208No Hit
CTCCCACTGCTTCACTTGACTAG240970.2994101630969674No Hit
CATTGCACTTGTCTCGGTCTGA239130.2971239253906205No Hit
TAGCTTATCAGACTGATGTTGACA217470.27021093152134085No Hit
TGAGGTAGTAGTTTGTGCTGTT205180.25494035466753445No Hit
AACATTCAACGCTGTCGGTGAGTT200150.2486904765898578No Hit
CTACGCCTGTCTGAGCGTCGCT192810.23957037617432167No Hit
CAAAGAATTCTCCTTTTGGGCT184580.22934443252038947No Hit
ACCCCCCACTGCTAAATTTGACTG179820.22343003497570935No Hit
CACCCGGCTGTGTGCACATGTGC169150.2101723413198823No Hit
TGTCAGTTTGTCAAATACCCCA163970.20373608516831865No Hit
TGTCAGTTTGTCAAATACCCCAA163710.2034130298402479No Hit
CTCCCACTGCTTCACTTGACTAGCCT161960.20123861897823314No Hit
CAAAGAATTCTCCTTTTGGGCTT159860.19862932594381547No Hit
AGCTCGGTCTGAGGCCCCTCAGT158930.1974737818857162No Hit
TAGCAGCACGTAAATATTGGC150390.18686265687908427No Hit
TTCAAGTAATCCAGGATAGGCTT148900.18501130134514027No Hit
CCCATAAAGTAGAAAGCACT148160.18409183618063116No Hit
TACGCCTGTCTGAGCGTCGCT147660.18347057593434124No Hit
TGTAAACATCCCCGACTGGAAGC144920.18006606978467243No Hit
TGAATACCGGGTGCTGTAGGCTTT128640.1598378361654724No Hit
TGTAAACATCCTACACTCAGCT128240.15934082796844046No Hit
CAACGGAATCCCAAAAGCAGCT127950.1589804970255923No Hit
TAGCAGCACGTAAATATTGGCGT127390.1582846855497476No Hit
CGCGACCTCAGATCAGACGTGGCG126100.1566818341143196No Hit
AACGGAATCCCAAAAGCAGCTG124340.15449499804737904No Hit
CAACACCAGTCGATGGGCTGT118310.14700259947712252No Hit
TTATAAAGCAATGAGACTGATT116830.14516366914810436No Hit
TTCACAGTGGCTAAGTTCCGC116120.14428147959837265No Hit
TCCTGTCTGAGCGTCGCT115880.14398327468015348No Hit
AACCGGGCGGAAACACCA114930.1428028802122026No Hit
GGGCTACGCCTGTCTGAGCGTCGCT110400.13717426238081587No Hit
CAAAGTGCTGTTCGTGCAGGTAG108410.13470164660058198No Hit
TATTGCACTTGTCCCGGCCTGTT107490.1335585277474085No Hit
ACTGGACTTGGAGTCAGAAGGCA107420.1334715513129279No Hit
TTCAAGTAATCCAGGATAGGC105970.13166989659868714No Hit
TTCACAGTGGCTAAGTTCCG105340.13088710868836181No Hit
CCCCCACTGCTAAATTTGACTGGCT104640.13001734434355594No Hit
ATCACATTGCCAGGGATTTCCA102600.127482602538693No Hit
TGAGGTAGTAGGTTGTGTGGTT102200.12698559434166107No Hit
GTGCGGTAACGCGACCGATCCCGGA101730.12640160971014855No Hit
GTCAGGATGGCCGAGCGGTCTAAGG100100.12437630130724339No Hit
GGAATACCGGGTGCTGTAGGCTTA99860.12407809638902423No Hit
ACGCCTGTCTGAGCGTCGCT99680.12385444270035986No Hit
TTCACAGTGGCTAAGTTCTGC97200.12077299187876181No Hit
ACCACAGGGTAGAACCACGGAC97140.12069844064920701No Hit
CACTAGATTGTGAGCTCCTGGA96240.11958017220588515No Hit
AGAGGTAGTAGGTTGCATAGTT95320.1184370533527117No Hit
TGAATACCGGGTGCTGTAGGCTT94200.11704543040102224No Hit
AGCAGCATTGTACAGGGCTATGT91290.11342969576761489No Hit
TGAGGTAGTAGGTTGTATAGT91150.1132557428986537No Hit
ACCGGGTGCTGTAGGCTT90920.11296996318536033No Hit
GCGTTCGCGCTTTCCCCTG90640.11262205744743796No Hit
ACTGGACTTGGAGTCAGAAGG89490.11119315888097113No Hit
ATCACATTGCCAGGGATTTCC86560.10755257383771215No Hit
TGATGTAGTAGGTTGTATAGTT83950.10430959535207875No Hit
CACTAGATTGTGAGCTCCTGGAA83780.10409836686834018No Hit
CGCGACCTCAGATCAGACGTGG83710.10401139043385958No Hit
TATTGCACTTGTCCCGGCCTGTAG83120.10327830334323747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)