FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9436468
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7157817.5852638932278476No Hit
CAACGGAATCCCAAAAGCAGCTG5686176.025739715325692No Hit
TAGCAGCACGTAAATATTGGCG3503263.7124695383908475No Hit
AAGCTGCCAGTTGAAGAACTGT3324413.52293887925016No Hit
TTCAAGTAATCCAGGATAGGCT2741012.9046990886844526No Hit
AACATTCAACGCTGTCGGTGAGT2633902.7911926369060964No Hit
CCCCCACTGCTAAATTTGACTG2368802.5102612545287073No Hit
TAGCTTATCAGACTGATGTTGAC2093982.21902940803699No Hit
TAGCTTATCAGACTGATGTTGACT1656671.755603897559977No Hit
TAGCTTATCAGACTGATGTTGA1396781.4801936487253495No Hit
TGTAAACATCCTTGACTGGAAGCT1210131.2823971850484737No Hit
AACATTCAACGCTGTCGGTGAG1187661.2585853096730684No Hit
TATTGCACTTGTCCCGGCCTGTAA1059531.1228035743882139No Hit
TGAGGTAGTAGGTTGTATAGTT910070.964418042852474No Hit
TATTGCACTTGTCCCGGCCTGTAT900040.9537890659937596No Hit
GGAATACCGGGTGCTGTAGGCTTT852600.9035160189172475No Hit
TATTGCACTTGTCCCGGCCTGT716910.7597228115434715No Hit
GCCTGTCTGAGCGTCGCT705110.7472181328861603No Hit
CATAAAGTAGAAAGCACTACT700420.7422480529791443No Hit
AGCAGCATTGTACAGGGCTATGA672290.7124381707223508No Hit
GGAATACCGGGTGCTGTAGGCTT608790.6451460440495321No Hit
TGTAAACATCCCCGACTGGAAGCT600020.6358523125389711No Hit
TTCAAGTAATTCAGGATAGGTT533830.5657095430196977No Hit
TGAGGTAGTAGATTGTATAGTT524130.5554302732759757No Hit
ACTGGACTTGGAGTCAGAAGGC509130.5395344953217666No Hit
TATTGCACTTGTCCCGGCCTGTA470390.4984809994586958No Hit
TTCTCACTACTGCACTTGACTAG453870.48097444933846006No Hit
TGAATACCGGGTGCTGTAGGCTTT438420.46460179804562474No Hit
CAACGGAATCCCAAAAGCAGCTGT436370.4624293750585495No Hit
CCCATAAAGTAGAAAGCACTAC413200.43787569671194776No Hit
CTTCTCACTACTGCACTTGACTAG391110.41446651437804904No Hit
CCCCCACTGCTAAATTTGACTGGCTT364090.38583291968986705No Hit
TGTAAACATCCTACACTCTCAGCT361770.383374372699616No Hit
TCCTGTCTGAGCGTCGCT342840.3633139009214041No Hit
TGAATACCGGGTGCTGTAGGCTT313180.3318826493132812No Hit
CGCCTGTCTGAGCGTCGCT296820.31454565415789043No Hit
AACATTCAACGCTGTCGGTGAGTT279940.29665760536675373No Hit
CATTGCACTTGTCTCGGTCTGA275590.2920478297600331No Hit
TTAATGCTAATCGTGATAGGGGTT274240.2906172097441543No Hit
CCCCCCACTGCTAAATTTGACTGGCT272320.28858255016601553No Hit
TCGTACGACTCTTAGCGG262350.27801715641911784No Hit
CCCCCCACTGCTAAATTTGACTGG256370.27168003960803977No Hit
CAACGGAATCCCAAAAGCAGCT251870.26691130622177706No Hit
CTCCCACTGCTTCACTTGACTAG246690.26142196423492353No Hit
TAGCTTATCAGACTGATGTTGACA245050.2596840258452633No Hit
CAAAGAATTCTCCTTTTGGGCT231880.24572753280146767No Hit
TGAGGTAGTAGTTTGTACAGTT214090.22687514014777563No Hit
CAAAGAATTCTCCTTTTGGGCTT208270.22070757830154247No Hit
TGATGTAGTAGGTTGTATAGTT200580.21255834280368457No Hit
CTACGCCTGTCTGAGCGTCGCT199400.2113078749379535No Hit
CACCCGGCTGTGTGCACATGTGC191740.203190430996004No Hit
CCCATAAAGTAGAAAGCACT183370.19432058689755533No Hit
TGTAAACATCCCCGACTGGAAGC178120.1887570646135821No Hit
TTCAAGTAATCCAGGATAGGCTT177060.18763376297148468No Hit
ACCCCCCACTGCTAAATTTGACTG176040.18655285007059844No Hit
CGCGACCTCAGATCAGACGTGGCG165110.1749701265346314No Hit
TCGTTTCCCGGCCAATGCACCA163950.17374085303950587No Hit
TAGCAGCACGTAAATATTGGC163740.17351831214814695No Hit
TGAGGTAGTAGTTTGTGCTGTT162340.17203470620575412No Hit
TGTCAGTTTGTCAAATACCCCAA157220.16660894733071738No Hit
AACCGGGCGGAAACACCA151020.1600386924429776No Hit
TACGCCTGTCTGAGCGTCGCT147880.1567111762578965No Hit
TGTCAGTTTGTCAAATACCCCA145710.15441158704718758No Hit
AGCTCGGTCTGAGGCCCCTCAGT142690.15121123708574014No Hit
CTCCCACTGCTTCACTTGACTAGCCT141580.15003494951712865No Hit
TTATAAAGCAATGAGACTGATT141390.14983360299637533No Hit
CAACACCAGTCGATGGGCTGT135830.14394156796801513No Hit
CACTAGATTGTGAGCTCCTGGA134660.14270169728758683No Hit
TGTAAACATCCTACACTCAGCT133260.14121809134519397No Hit
GGAATACCGGGTGCTGTAGGCTTA132510.14042330244748352No Hit
TATTGCACTTGTCCCGGCCTGTT131040.13886551620797102No Hit
ACTTGACTTGGAGTCAGAAGGC126150.13368349259489887No Hit
ACTGGACTTGGAGTCAGAAGGCA125560.1330582586620333No Hit
TAGCAGCACGTAAATATTGGCGT125130.13260257969401262No Hit
TTCACAGTGGCTAAGTTCCG121170.1284060943141014No Hit
ATCACATTGCCAGGGATTTCCA119930.12709204333655347No Hit
TGATGTAGTAGATTGTATAGTT118580.12566142332067465No Hit
TATTGCACTTGTCCCGGCCTGTAG117770.12480305131114734No Hit
AACGGAATCCCAAAAGCAGCTG115950.12287436358603664No Hit
AGCAGCATTGTACAGGGCTATGT114860.12171927038803078No Hit
CACTAGATTGTGAGCTCCTGGAA114170.12098806460213715No Hit
ACCACAGGGTAGAACCACGGAC111980.11866728102082262No Hit
CAAAGTGCTGTTCGTGCAGGTAG108360.11483109994120681No Hit
TTCAAGTAATCCAGGATAGGC106300.11264807976882876No Hit
GTGCGGTAACGCGACCGATCCCGGA104890.11115387664113312No Hit
TTCACAGTGGCTAAGTTCTGC103540.10972325662525427No Hit
CCCCCACTGCTAAATTTGACTGGCT102100.1081972619416502No Hit
TGTAAACATCCTTGACTGGAAGC100260.10624737984593388No Hit
TTCACAGTGGCTAAGTTCCGC100110.1060884220663918No Hit
GGAATACCGGGTGCTGTAGGCT100050.10602483895457496No Hit
CCCGCCACTGCTAAATTTGACTG99910.10587647836033567No Hit
ACTGGACTTGGAGTCAGAAGG98400.10427630337961195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)