FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9989380
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6462846.4697108329045445No Hit
CCCCCCACTGCTAAATTTGACTG5583965.589896470051194No Hit
AAGCTGCCAGTTGAAGAACTGT4504824.509609204975684No Hit
TAGCAGCACGTAAATATTGGCG3643983.6478540209702706No Hit
TTCAAGTAATCCAGGATAGGCT3364933.368507354810809No Hit
AACATTCAACGCTGTCGGTGAGT2277852.280271648490697No Hit
CCCCCACTGCTAAATTTGACTG2249302.251691296156518No Hit
GCCTGTCTGAGCGTCGCT2196142.19847478021659No Hit
TAGCTTATCAGACTGATGTTGAC1878451.8804470347509052No Hit
TAGCTTATCAGACTGATGTTGA1652731.6544870652633097No Hit
TAGCTTATCAGACTGATGTTGACT1231261.232568988265538No Hit
TATTGCACTTGTCCCGGCCTGT1158971.1602021346670164No Hit
CATAAAGTAGAAAGCACTACT1151731.1529544376127447No Hit
TGAGGTAGTAGGTTGTATAGTT1110341.1115204347016532No Hit
AACATTCAACGCTGTCGGTGAG1064181.0653113606650262No Hit
TATTGCACTTGTCCCGGCCTGTAA899280.9002360506858283No Hit
TATTGCACTTGTCCCGGCCTGTAT844030.8449273128062003No Hit
TGTAAACATCCTTGACTGGAAGCT769190.7700077482286188No Hit
TCCTGTCTGAGCGTCGCT762550.763360689051773No Hit
TTCAAGTAATTCAGGATAGGTT638060.6387383401172044No Hit
TGAGGTAGTAGATTGTATAGTT608690.6093371160172102No Hit
AGCAGCATTGTACAGGGCTATGA602280.6029203013600444No Hit
CGCCTGTCTGAGCGTCGCT545220.5457996392168483No Hit
CCCATAAAGTAGAAAGCACTAC526980.5275402477431032No Hit
TATTGCACTTGTCCCGGCCTGTA519020.5195717852359205No Hit
CATTGCACTTGTCTCGGTCTGA458240.45872716825268434No Hit
CAACGGAATCCCAAAAGCAGCTGT458090.4585770087833279No Hit
TTCTCACTACTGCACTTGACTAG429370.4298264757172117No Hit
TGATGTAGTAGGTTGTATAGTT423470.4239202032558577No Hit
ACTGGACTTGGAGTCAGAAGGC408880.40931469220311967No Hit
TGTAAACATCCCCGACTGGAAGCT387930.3883424196496679No Hit
AACCGGGCGGAAACACCA332500.33285349040681206No Hit
TGTAAACATCCTACACTCTCAGCT314450.3147843009275851No Hit
CTTCTCACTACTGCACTTGACTAG307080.3074064656665379No Hit
CTCCCACTGCTTCACTTGACTAG288160.28846635126504344No Hit
CTACGCCTGTCTGAGCGTCGCT283160.28346103561982827No Hit
CCCATAAAGTAGAAAGCACT268500.2687854501480572No Hit
TGAGGTAGTAGTTTGTACAGTT235870.2361207602473827No Hit
TGATGTAGTAGATTGTATAGTT234610.2348594207047885No Hit
CACCCGGCTGTGTGCACATGTGC231050.23129563596539526No Hit
CCCCCACTGCTAAATTTGACTGGCTT228440.22868286119859293No Hit
TTAATGCTAATCGTGATAGGGGTT223600.22383771565402458No Hit
AACATTCAACGCTGTCGGTGAGTT217530.2177612624607333No Hit
TGAGGTAGTAGTTTGTGCTGTT213020.21324646774874917No Hit
CAACGGAATCCCAAAAGCAGCT208530.20875169429934592No Hit
AGCTCGGTCTGAGGCCCCTCAGT207660.20788076937707844No Hit
ACCGGGTGCTGTAGGCTT203940.20415681453703835No Hit
CCCCCCACTGCTAAATTTGACTGG198720.19893126500343364No Hit
CAACACCAGTCGATGGGCTGT196020.19622839455501742No Hit
CCCCCCACTGCTAAATTTGACTGGCT186230.18642798652168605No Hit
TAGCAGCACGTAAATATTGGC178530.1787198004280546No Hit
TTCACAGTGGCTAAGTTCCG177930.17811916255062876No Hit
TATTGCACTTGTCCCGGCCTGTT174810.17499584558801445No Hit
ACTTGACTTGGAGTCAGAAGGC174700.17488572864381974No Hit
TAGCAGCACGTAAATATTGGCGT173110.17329403826864131No Hit
TTCAAGTAATCCAGGATAGGCTT172680.1728635811231528No Hit
TAGCTTATCAGACTGATGTTGACA170760.17094153991539016No Hit
ACGCCTGTCTGAGCGTCGCT169400.1695800940598916No Hit
TACGCCTGTCTGAGCGTCGCT164310.16448468273306252No Hit
TTATAAAGCAATGAGACTGATT162050.16222228006142525No Hit
TTCAAGTAATCCAGGATAGGC156880.1570467836842727No Hit
TCGTACGACTCTTAGCGG152940.1531025949558431No Hit
TGTAAACATCCTACACTCAGCT152330.15249194644712685No Hit
CACTAGATTGTGAGCTCCTGGA149650.1498090972612915No Hit
TTCACAGTGGCTAAGTTCTGC143430.14358248459864376No Hit
TTCACAGTGGCTAAGTTCCGC142450.14260144273218156No Hit
ACCGGGTGCTGTAGGCTTT142340.14249132578798684No Hit
GGAATACCGGGTGCTGTAGGCTTT134710.13485321411338844No Hit
ACCACAGGGTAGAACCACGGAC134430.13457291643725636No Hit
ATCCCGGACGAGCCCCCA132760.1329011410117545No Hit
CAAAGTGCTGTTCGTGCAGGTAG132240.13238058818465212No Hit
TGCAAGTAATCCAGGATAGGCT130760.13089901475366839No Hit
CAAAGAATTCTCCTTTTGGGCT130250.13038847255785643No Hit
CAAAGTGCTGTTCGTGCAGGT126670.12680466655588235No Hit
CAAAGAATTCTCCTTTTGGGCTT126100.12623406057232783No Hit
ACCCCCCACTGCTAAATTTGACTG125340.1254732525942551No Hit
TGTCAGTTTGTCAAATACCCCA124930.12506281671134745No Hit
GCGTTCGCGCTTTCCCCTG123890.12402171105714269No Hit
TGTAAACATCCCCGACTGGAAGC120780.12090840472581883No Hit
AACGGAATCCCAAAAGCAGCTG116880.11700425852255096No Hit
GGGCTACGCCTGTCTGAGCGTCGCT115290.11541256814737251No Hit
CTCCCACTGCTTCACTTGACTAGCCT114210.11433141996800601No Hit
GGAATACCGGGTGCTGTAGGCTT112740.11285985716831275No Hit
AGCAGCATTGTACAGGGCTATG111560.11167860267604196No Hit
AGCAGCATTGTACAGGGCTATGT111310.11142833689378119No Hit
TAGCAGCACATAATGGTTTGT110960.11107796479861613No Hit
TCCCACTGCTTCACTTGACTAG109780.10989671030634533No Hit
ACCGGGTGCTGTAGGCTTA108980.1090958598031109No Hit
TCGTTTCCCGGCCAATGCACCA106170.10628287241049995No Hit
TGTCAGTTTGTCAAATACCCCAA103580.10369011890627848No Hit
TTCACCACCTTCTCCACCCAGC102010.10211844979368088No Hit
CCCATAAAGTAGAAAGCACTA100400.1005067381559216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)