FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7564184
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG84651511.191094769772919No Hit
CAACGGAATCCCAAAAGCAGCTG5075366.709725728512157No Hit
CCCCCACTGCTAAATTTGACTG3798275.021387634145336No Hit
GCCTGTCTGAGCGTCGCT2464903.25864627301504No Hit
AACATTCAACGCTGTCGGTGAGT2186442.890516677013674No Hit
TTCAAGTAATCCAGGATAGGCT1581802.0911707065824943No Hit
AACATTCAACGCTGTCGGTGAG1524192.0150091536641623No Hit
TAGCTTATCAGACTGATGTTGAC1481751.958902639068537No Hit
TAGCAGCACGTAAATATTGGCG1427841.8876325589118403No Hit
AAGCTGCCAGTTGAAGAACTGT1305501.725896673058191No Hit
TCCTGTCTGAGCGTCGCT1243561.6440107749890802No Hit
TAGCTTATCAGACTGATGTTGACT1229311.6251719947584564No Hit
TATTGCACTTGTCCCGGCCTGTAA1087751.4380268909376082No Hit
TGAGGTAGTAGGTTGTATAGTT1065771.4089688986941618No Hit
TAGCTTATCAGACTGATGTTGA1028581.3598029873413973No Hit
TATTGCACTTGTCCCGGCCTGTAT890581.1773642735290417No Hit
TGAGGTAGTAGATTGTATAGTT665600.8799362892282895No Hit
TATTGCACTTGTCCCGGCCTGT662760.8761817533788179No Hit
GGAATACCGGGTGCTGTAGGCTTT560370.7408201598480417No Hit
TATTGCACTTGTCCCGGCCTGTA514840.6806286044866174No Hit
GGAATACCGGGTGCTGTAGGCTT429980.5684420156886718No Hit
CATAAAGTAGAAAGCACTACT428150.5660227197011601No Hit
TTCAAGTAATTCAGGATAGGTT403260.5331176502316707No Hit
ACTGGACTTGGAGTCAGAAGGC401090.5302488675579548No Hit
AGCAGCATTGTACAGGGCTATGA395170.5224225111393377No Hit
TGTAAACATCCTTGACTGGAAGCT385380.5094799386159828No Hit
CATTGCACTTGTCTCGGTCTGA378700.5006488472517326No Hit
TGTAAACATCCCCGACTGGAAGCT320150.4232445958480121No Hit
CACCCGGCTGTGTGCACATGTGC317180.41931819744205057No Hit
TGAATACCGGGTGCTGTAGGCTTT313970.41507451431641534No Hit
CAACACCAGTCGATGGGCTGT294890.38985037910235926No Hit
CAACGGAATCCCAAAAGCAGCT254480.33642756442730637No Hit
TGAATACCGGGTGCTGTAGGCTT243200.3215151826026442No Hit
CAACGGAATCCCAAAAGCAGCTGT239740.3169409945606823No Hit
TTAATGCTAATCGTGATAGGGGTT235490.31132241098312785No Hit
TTCTCACTACTGCACTTGACTAG233700.30895599578222843No Hit
TGATGTAGTAGGTTGTATAGTT229310.30315232945153103No Hit
TGAGGTAGTAGTTTGTACAGTT213440.28217187736311017No Hit
ACCGGGTGCTGTAGGCTT206430.2729045195093086No Hit
CCCCCACTGCTAAATTTGACTGGCTT199790.26412630893167066No Hit
CACTAGATTGTGAGCTCCTGGA193700.25607520916995147No Hit
CTTCTCACTACTGCACTTGACTAG176940.2339181595794074No Hit
TGAGGTAGTAGTTTGTGCTGTT176200.23293986502708025No Hit
TGTCAGTTTGTCAAATACCCCAA174050.23009752274667036No Hit
CCCATAAAGTAGAAAGCACTAC169710.22435995739923828No Hit
AACATTCAACGCTGTCGGTGAGTT166800.22051288017319515No Hit
TAGCTTATCAGACTGATGTTGACA158950.2101350258005358No Hit
TTATAAAGCAATGAGACTGATT150180.19854091333579407No Hit
CCCATAAAGTAGAAAGCACT148190.19591009420183328No Hit
ACCGGGTGCTGTAGGCTTT147210.19461451492983248No Hit
ACCCCCCACTGCTAAATTTGACTG145600.19248606326868833No Hit
CACTAGATTGTGAGCTCCTGGAA140030.18512241373292876No Hit
TGATGTAGTAGATTGTATAGTT139060.18384005465758105No Hit
TATTGCACTTGTCCCGGCCTGTAG134310.17756046124737315No Hit
ACCGGGTGCTGTAGGCTTA130840.17297305300875812No Hit
CTCCCACTGCTTCACTTGACTAG129260.1708842619375732No Hit
AACGGAATCCCAAAAGCAGCTG122060.1613657203473633No Hit
CGCCCCACTGCTAAATTTGACTG118110.15614374266940095No Hit
CCCCCCACTGCTAAATTTGACTGG117700.1556017146066251No Hit
TAGCAGCACGTAAATATTGGC112000.14806620251437563No Hit
CAAAGAATTCTCCTTTTGGGCT110710.1463607971461297No Hit
TTAGGTAGTAGGTTGTATAGTT109830.1451974198406596No Hit
ACTGGACTTGGAGTCAGAAGGCA109020.144126583911761No Hit
TATTGCACTTGTCCCGGCCTGTT107630.14228897657698436No Hit
TGAGAACTGAATTCCATAGGCTGT106890.14131068202465727No Hit
CTCCCACTGCTTCACTTGACTAGCCT104830.13858732151412498No Hit
TGTCAGTTTGTCAAATACCCCA100910.13340500442612185No Hit
CCCCCCACTGCTAAATTTGACTGGCT95800.12664948393640346No Hit
TGAGAACTGAATTCCATGGGTT94760.12527458348448425No Hit
CGCCTGTCTGAGCGTCGCT93770.12396578401583039No Hit
ACTGGACTTGGAGTCAGAAGGCT92970.12290816828358486No Hit
TGTAAACATCCTACACTCTCAGCT91880.12146716684840031No Hit
CCCTGTCTGAGCGTCGCT91610.12111022153876744No Hit
ACTTGACTTGGAGTCAGAAGGC90080.11908753145084784No Hit
ACTGGACTTGGAGTCAGAAGG88550.11706484136292823No Hit
GGAATACCGGGTGCTGTAGGCTTA88130.11650959310349933No Hit
CTCCCCACTGCTAAATTTGACTG87570.11576926209092746No Hit
ACCACAGGGTAGAACCACGGAC87200.11528011481476388No Hit
CAAAGAATTCTCCTTTTGGGCTT86680.1145926645888043No Hit
TTCACAGTGGCTAAGTTCTG86650.11455300399884508No Hit
CAAAGTGCTGTTCGTGCAGGTAG85510.11304590158039518No Hit
TCGTTTCCCGGCCAATGCACCA85460.11297980059712984No Hit
TTCACAGTGGCTAAGTTCCGC85290.11275505725402767No Hit
TTCACAGTGGCTAAGTTCTGC83870.11087778932929183No Hit
AACCGGGCGGAAACACCA83710.11066626618284271No Hit
TCGTACGACTCTTAGCGG82570.10915916376439283No Hit
TGAGGTAGTAGGTTGTATAGT82430.10897408101124986No Hit
TGAGGTAGTAAGTTGTATTGTT81650.10794290567231045No Hit
TATTGCACTTGTCCCGGCCTGTAAT81530.10778426331247364No Hit
AATGCACCCGGGCAAGGATTCT79380.10494192103206375No Hit
TTCAAGTAATCCAGGATAGGCTT79370.10492870083541066No Hit
GGAATACCGGGTGCTGTAGGCT77350.10225822111149067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)