FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5681825
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG3424466.027042367549159No Hit
CAACGGAATCCCAAAAGCAGCTG2469934.3470715835141No Hit
AACATTCAACGCTGTCGGTGAGT1833073.226199328560806No Hit
AAGCTGCCAGTTGAAGAACTGT1799023.1662714004743195No Hit
TAGCAGCACGTAAATATTGGCG1561772.748711901545718No Hit
CCCCCACTGCTAAATTTGACTG1431662.5197185763377083No Hit
TTCAAGTAATCCAGGATAGGCT1413962.4885666137200637No Hit
TAGCTTATCAGACTGATGTTGAC1145252.0156375812349028No Hit
TCGTACGACTCTTAGCGG1103241.941700069959916No Hit
TAGCTTATCAGACTGATGTTGA904821.5924812890224533No Hit
CGCCTGTCTGAGCGTCGCT803481.4141231030522763No Hit
AACATTCAACGCTGTCGGTGAG777901.3691023570771714No Hit
TATTGCACTTGTCCCGGCCTGT728991.2830208603749675No Hit
TAGCTTATCAGACTGATGTTGACT722401.271422474293031No Hit
TGAGGTAGTAGGTTGTATAGTT640891.1279650464419442No Hit
CATAAAGTAGAAAGCACTACT539350.949254860894167No Hit
TGTAAACATCCTTGACTGGAAGCT433380.7627478847025384No Hit
TATTGCACTTGTCCCGGCCTGTAA425880.7495479005425193No Hit
AGCAGCATTGTACAGGGCTATGA379390.6677255987292815No Hit
TATTGCACTTGTCCCGGCCTGTAT369600.6504952194057367No Hit
TGAGGTAGTAGATTGTATAGTT353800.6226872527752967No Hit
ACTGGACTTGGAGTCAGAAGGC349840.6157176611388067No Hit
TATTGCACTTGTCCCGGCCTGTA310880.547148143422228No Hit
TTCAAGTAATTCAGGATAGGTT305610.5378729545524545No Hit
CATTGCACTTGTCTCGGTCTGA279710.4922890092531889No Hit
TGTAAACATCCCCGACTGGAAGCT256500.45143945827265003No Hit
ACCGGGTGCTGTAGGCTT236760.41669709996348003No Hit
CCCATAAAGTAGAAAGCACTAC229960.4047291143250628No Hit
CTACGCCTGTCTGAGCGTCGCT201310.3543051748337902No Hit
TACGCCTGTCTGAGCGTCGCT197470.34754678294386043No Hit
TTCTCACTACTGCACTTGACTAG197420.3474587830494604No Hit
AACATTCAACGCTGTCGGTGAGTT190630.3355083973899231No Hit
ACGCCTGTCTGAGCGTCGCT169700.2986716415940301No Hit
CCCATAAAGTAGAAAGCACT168940.2973340431991482No Hit
CAACACCAGTCGATGGGCTGT166980.2938844473386632No Hit
CTCCCACTGCTTCACTTGACTAG163780.2882524540970551No Hit
CAACGGAATCCCAAAAGCAGCTGT155560.2737852714576743No Hit
GCCTGTCTGAGCGTCGCT149580.2632604840874191No Hit
CTTCTCACTACTGCACTTGACTAG148740.261782085861497No Hit
TGTAAACATCCTACACTCTCAGCT143160.2519612976464428No Hit
TGATGTAGTAGGTTGTATAGTT142980.2516444980266024No Hit
TGAGGTAGTAGTTTGTGCTGTT134990.2375821149014621No Hit
TGAGGTAGTAGTTTGTACAGTT134200.23619171656994012No Hit
GCGTTCGCGCTTTCCCCTG133130.2343085188297774No Hit
ACCGGGTGCTGTAGGCTTT129350.22765572681312782No Hit
ATCCCGGACGAGCCCCCA117240.20634215238941714No Hit
CCCCCCACTGCTAAATTTGACTGG117050.20600775279069664No Hit
TTATAAAGCAATGAGACTGATT115360.20303335635997238No Hit
CCCCCCACTGCTAAATTTGACTGGCT110430.19435656677211988No Hit
CCCCCACTGCTAAATTTGACTGGCTT109630.19294856846171785No Hit
TACCTGGTTGATCCTGCC107450.18911177306587232No Hit
TAGCAGCACGTAAATATTGGC106810.1879853744175507No Hit
TAGCTTATCAGACTGATGTTGACA103900.1828637805634633No Hit
TGTCAGTTTGTCAAATACCCCAA103660.18244138107034272No Hit
TATTGCACTTGTCCCGGCCTGTT102010.1795373845551385No Hit
TTCACAGTGGCTAAGTTCCG100190.1763341883989739No Hit
CACCCGGCTGTGTGCACATGTGC100120.17621098854681375No Hit
TAGTGGGTTATCAGAACT97620.17181099382680742No Hit
ATCCCACCGCTGCCACCA97470.17154699414360702No Hit
CCTCGACACAAGGGTTTGT96810.17038539553752535No Hit
ACCACAGGGTAGAACCACGGAC95040.16727019927576087No Hit
ACCGGGTGCTGTAGGCTTA92250.1623598051682338No Hit
TTCAAGTAATCCAGGATAGGCTT86580.15238061714325943No Hit
TGAGGTAGTAGGTTGTGTGGTT86530.1522926172488593No Hit
ACTGGACTTGGAGTCAGAAGG84480.1486846215784541No Hit
ACTTGACTTGGAGTCAGAAGGC84260.14829742204309354No Hit
TAGCAGCACGTAAATATTGGCGT82150.1445838264994082No Hit
CAACGGAATCCCAAAAGCAGCT80760.14213742943508467No Hit
TGATGTAGTAGATTGTATAGTT80520.14171502994196405No Hit
TCCTGTCTGAGCGTCGCT78800.1386878335745997No Hit
TGTAAACATCCTACACTCAGCT77090.13567823718611538No Hit
CCAAGTCCTTCTGATCGAGGCCC76150.134023839171393No Hit
TCGTTTCCCGGCCAATGCACCA76020.13379503944595267No Hit
AGCAGCATTGTACAGGGCTATG74980.13196464164243002No Hit
TGTAAACATCCCCGACTGGAAGC73450.12927184487378615No Hit
CTCCCACTGCTTCACTTGACTAGCCT73150.1287438455073854No Hit
CAAAGTGCTGTTCGTGCAGGTAG72840.12819824616210462No Hit
TTCAAGTAATCCAGGATAGGC71980.12668464797842244No Hit
TCGTTCGCGCTTTCCCCTG71730.12624464850642178No Hit
CACTAGATTGTGAGCTCCTGGA71150.12522384973138032No Hit
TTCACAGTGGCTAAGTTCCGC69500.12231985321617615No Hit
TGTCAGTTTGTCAAATACCCCA68260.12013745583505299No Hit
ACTGGACTTGGAGTCAGAAGGCA66690.11737425915088902No Hit
AGCAGCATTGTACAGGGCTATGT66520.1170750595099286No Hit
TTCACCACCTTCTCCACCCAGC66380.11682865980560822No Hit
TGAGGTAGTAGGTTGTATAGT65210.11476946227664526No Hit
AGCTCGGTCTGAGGCCCCTCAGT64960.11432946280464465No Hit
AACGGAATCCCAAAAGCAGCTG64940.11429426284688458No Hit
CTCGACACAAGGGTTTGT64870.1141710629947244No Hit
CAAAGTGCTGTTCGTGCAGGT63690.11209426548688141No Hit
GTCAGGATGGCCGAGCGGTCTAAGG63620.11197106563472124No Hit
TAGCTTATCAGACTGATGTTG59730.10512467385039138No Hit
GGGCTACGCCTGTCTGAGCGTCGCT57060.10042547948942461No Hit
ACTGGACTTGGAGTCAGAAGGCT56840.10003827995406404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)