FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7272218
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4639206.37934671375363No Hit
CCCCCCACTGCTAAATTTGACTG4437616.102141052427196No Hit
GGAATACCGGGTGCTGTAGGCTTT3749015.155249746363489No Hit
GGAATACCGGGTGCTGTAGGCTT2305263.169954476062186No Hit
TGAATACCGGGTGCTGTAGGCTTT2039242.804151360699033No Hit
TTCAAGTAATCCAGGATAGGCT1718722.3634054974699605No Hit
TAGCAGCACGTAAATATTGGCG1694772.3304719412976893No Hit
AACATTCAACGCTGTCGGTGAGT1477842.0321723028655083No Hit
CCCCCACTGCTAAATTTGACTG1472752.0251730627437183No Hit
AAGCTGCCAGTTGAAGAACTGT1362541.8736237004996275No Hit
TATTGCACTTGTCCCGGCCTGTAT1355411.8638192639439575No Hit
TATTGCACTTGTCCCGGCCTGTAA1306491.7965495533824756No Hit
TGAATACCGGGTGCTGTAGGCTT1251761.7212905333695991No Hit
TAGCTTATCAGACTGATGTTGACT1224021.6831453622539918No Hit
AACATTCAACGCTGTCGGTGAG1194371.6423737572223496No Hit
TAGCTTATCAGACTGATGTTGAC1141771.570043692309554No Hit
TAGCTTATCAGACTGATGTTGA833251.1457989845738947No Hit
TATTGCACTTGTCCCGGCCTGT820421.1281564991588535No Hit
TGAGGTAGTAGGTTGTATAGTT713750.9814749777853194No Hit
GCCTGTCTGAGCGTCGCT663940.9129814315247425No Hit
GGAATACCGGGTGCTGTAGGCTTA611450.8408026272039699No Hit
TATTGCACTTGTCCCGGCCTGTA609480.838093687510468No Hit
TGTAAACATCCTTGACTGGAAGCT571740.7861975534836826No Hit
TGAGGTAGTAGATTGTATAGTT491710.6761485973055263No Hit
AGCAGCATTGTACAGGGCTATGA484430.6661378963061889No Hit
TTCAAGTAATTCAGGATAGGTT479990.6600324687736259No Hit
CATAAAGTAGAAAGCACTACT408180.5612868041084577No Hit
TGTAAACATCCCCGACTGGAAGCT405320.5573540287158608No Hit
GGAATACCGGGTGCTGTAGGCT403550.5549201082805824No Hit
TGAATACCGGGTGCTGTAGGCTTA334400.4598321997497875No Hit
TCCTGTCTGAGCGTCGCT309360.4253997886201981No Hit
CAACGGAATCCCAAAAGCAGCTGT293270.403274489296113No Hit
ACTGGACTTGGAGTCAGAAGGC248900.3422614668592168No Hit
CATTGCACTTGTCTCGGTCTGA237820.3270253999536318No Hit
TGATGTAGTAGGTTGTATAGTT237070.3259940777352934No Hit
TGAATACCGGGTGCTGTAGGCT221750.30492760255536894No Hit
CCCATAAAGTAGAAAGCACTAC215490.29631949977297156No Hit
TCGTACGACTCTTAGCGG213660.29380307356022606No Hit
TTCTCACTACTGCACTTGACTAG204200.2807946626462518No Hit
CACCCGGCTGTGTGCACATGTGC204020.2805471453138506No Hit
ATCCCGGACGAGCCCCCA189900.2611307856832675No Hit
CAACACCAGTCGATGGGCTGT181400.24944246720876628No Hit
TGAGGTAGTAGTTTGTACAGTT177470.24403833878467338No Hit
TAGCTTATCAGACTGATGTTGACA176610.24285575597431208No Hit
TATTGCACTTGTCCCGGCCTGTAG175630.24150816160901667No Hit
TATTGCACTTGTCCCGGCCTGTT166280.22865101128706536No Hit
CTCCCACTGCTTCACTTGACTAG163860.22532327826256032No Hit
TGATGTAGTAGATTGTATAGTT163120.22430570700713318No Hit
AGCTCGGTCTGAGGCCCCTCAGT160890.22123924227794053No Hit
CCCATAAAGTAGAAAGCACT159850.219809142135178No Hit
CCCCCACTGCTAAATTTGACTGGCTT158510.21796651310508017No Hit
CTTCTCACTACTGCACTTGACTAG153010.21040348350393234No Hit
ACCGGGTGCTGTAGGCTTA152380.20953717284052817No Hit
CACTAGATTGTGAGCTCCTGGAA146130.200942821021042No Hit
CAAAGAATTCTCCTTTTGGGCT142930.1965425128894651No Hit
TGTAAACATCCTACACTCTCAGCT133140.18308032019942197No Hit
AACCGGGCGGAAACACCA132920.18277779901537605No Hit
CACTAGATTGTGAGCTCCTGGA132870.18270904420082018No Hit
ACCGGGTGCTGTAGGCTT129790.1784737476241774No Hit
TATTGCACTTGTCCCGGCCTGTTT125350.1723683200916144No Hit
ACCCCCCACTGCTAAATTTGACTG118200.16253638161012224No Hit
CAACGGAATCCCAAAAGCAGCT117630.1617525767241851No Hit
AACATTCAACGCTGTCGGTGAGTT117270.16125754205938272No Hit
ACCGGGTGCTGTAGGCTTT116900.16074875643166914No Hit
TATTGCACTTGTCCCGGCCTGTAAT112570.15479458949112912No Hit
CCCCCCACTGCTAAATTTGACTGGCT110730.15226441231547239No Hit
CCCCCCACTGCTAAATTTGACTGG110410.1518243815023147No Hit
CGCCTGTCTGAGCGTCGCT107240.1474653262594713No Hit
TAGCAGCACGTAAATATTGGC102670.14118113620906306No Hit
TGTAAACATCCCCGACTGGAAGC100450.13812842244278156No Hit
TGAGGTAGTAGTTTGTGCTGTT99800.13723460985355498No Hit
AGCAGCATTGTACAGGGCTATGT99550.13689083578077554No Hit
CGAATACCGGGTGCTGTAGGCTTT98870.13595577030281544No Hit
TTAATACCGGGTGCTGTAGGCTTT98090.13488319519574357No Hit
TATTGCACTTGTCCCGGCCTGTAAA96580.13280679979615573No Hit
GGAATACCGGGTGCTGTAGGCTA96480.13266929016704396No Hit
CAAAGAATTCTCCTTTTGGGCTT95500.13132169580174852No Hit
TTCAAGTAATCCAGGATAGGCTT90150.12396493064426838No Hit
TCAGTGCACTACAGAACTTTGT90140.12395117968135719No Hit
TTATAAAGCAATGAGACTGATT89150.12258983435315057No Hit
ACTTGACTTGGAGTCAGAAGGC88810.12212230161417054No Hit
AACGGAATCCCAAAAGCAGCTG88700.12197104102214759No Hit
TGAGAACTGAATTCCATAGGCTGT85580.11768074059386009No Hit
TCGTTTCCCGGGCGGCGCACCA81750.11241412179887897No Hit
TATTGCACTTGTCCCGGCCTGA81370.11189158520825422No Hit
CTCCCACTGCTTCACTTGACTAGCCT78950.10856385218374917No Hit
TTCAAGTAATCCAGGATAGGC78400.10780754922363439No Hit
ACTGGACTTGGAGTCAGAAGGCA76630.1053736287883559No Hit
TGTCAGTTTGTCAAATACCCCA73410.10094581873095662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)