FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11366897
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5729545.040548885065115No Hit
CCCCCCACTGCTAAATTTGACTG5122824.506788440152136No Hit
AAGCTGCCAGTTGAAGAACTGT3484593.0655595805961817No Hit
AACATTCAACGCTGTCGGTGAGT3417413.006458138927449No Hit
GCCTGTCTGAGCGTCGCT3093962.72190378781474No Hit
TTCAAGTAATCCAGGATAGGCT3035062.6700866560152696No Hit
TAGCAGCACGTAAATATTGGCG2901722.5527811151979294No Hit
TAGCTTATCAGACTGATGTTGAC2352282.0694126110230435No Hit
CCCCCACTGCTAAATTTGACTG2178471.9165036860983258No Hit
CGCCTGTCTGAGCGTCGCT1931781.6994787583629902No Hit
TAGCTTATCAGACTGATGTTGA1723601.5163329095002798No Hit
AACATTCAACGCTGTCGGTGAG1703421.4985796035628722No Hit
TAGCTTATCAGACTGATGTTGACT1673251.472037619413636No Hit
TGAGGTAGTAGGTTGTATAGTT1348261.1861284570450494No Hit
TATTGCACTTGTCCCGGCCTGTAA1228601.0808578629682315No Hit
TATTGCACTTGTCCCGGCCTGT1209901.0644065834325762No Hit
CATAAAGTAGAAAGCACTACT1191531.0482456205946091No Hit
TCCTGTCTGAGCGTCGCT1067430.939068947312534No Hit
TGTAAACATCCTTGACTGGAAGCT940110.8270594868590785No Hit
TATTGCACTTGTCCCGGCCTGTAT939810.826795562588453No Hit
AGCAGCATTGTACAGGGCTATGA872240.7673510193679066No Hit
TGAGGTAGTAGATTGTATAGTT750850.6605584619971484No Hit
GGAATACCGGGTGCTGTAGGCTTT749030.6589573214220205No Hit
TTCAAGTAATTCAGGATAGGTT666230.586114222729387No Hit
TATTGCACTTGTCCCGGCCTGTA634000.5577599585885225No Hit
TCGTACGACTCTTAGCGG582460.5124177688950644No Hit
GGAATACCGGGTGCTGTAGGCTT566720.49857054216291397No Hit
ACCGGGTGCTGTAGGCTT553610.4870370515365803No Hit
ACTGGACTTGGAGTCAGAAGGC533170.46905501123129734No Hit
TGTAAACATCCCCGACTGGAAGCT515390.45341309945889363No Hit
CAACACCAGTCGATGGGCTGT486430.42793560986784696No Hit
TACGCCTGTCTGAGCGTCGCT433400.38128259629694894No Hit
CCCATAAAGTAGAAAGCACTAC417800.36755853422442375No Hit
CATTGCACTTGTCTCGGTCTGA408300.3592009323212835No Hit
ACGCCTGTCTGAGCGTCGCT398440.35052662129339257No Hit
ACCGGGTGCTGTAGGCTTT371830.32711653848891215No Hit
CAACGGAATCCCAAAAGCAGCTGT366770.322665015791029No Hit
CCCATAAAGTAGAAAGCACT361560.3180815309578331No Hit
CTACGCCTGTCTGAGCGTCGCT328110.2886539747830917No Hit
TGAGGTAGTAGTTTGTACAGTT328010.28856600002621646No Hit
AACATTCAACGCTGTCGGTGAGTT326310.2870704291593387No Hit
GCGTTCGCGCTTTCCCCTG301660.2653846515896115No Hit
TTCTCACTACTGCACTTGACTAG301500.26524389197861126No Hit
AACCGGGCGGAAACACCA301420.2651735121731111No Hit
ACCGGGTGCTGTAGGCTTA277140.24381324120382195No Hit
ATCCCGGACGAGCCCCCA262250.23071379990511043No Hit
TGAATACCGGGTGCTGTAGGCTTT261520.23007158417992177No Hit
TGATGTAGTAGGTTGTATAGTT260830.22946455835748314No Hit
TGTAAACATCCTACACTCTCAGCT259590.22837367137223114No Hit
CACCCGGCTGTGTGCACATGTGC249710.2196817653929652No Hit
TTATAAAGCAATGAGACTGATT247850.21804543491508718No Hit
TAGCTTATCAGACTGATGTTGACA244800.21536220483039475No Hit
TGAGGTAGTAGTTTGTGCTGTT231350.2035296000306856No Hit
TTAATGCTAATCGTGATAGGGGTT224650.1976352913200498No Hit
CTTCTCACTACTGCACTTGACTAG219740.19331573075747938No Hit
CAACGGAATCCCAAAAGCAGCT219620.19321016104922917No Hit
TGAATACCGGGTGCTGTAGGCTT195720.17218419415606562No Hit
TTCACAGTGGCTAAGTTCCG189460.16667697437568052No Hit
CTCCCACTGCTTCACTTGACTAG185880.16352747807954976No Hit
ACCACAGGGTAGAACCACGGAC183020.16101140003292014No Hit
TTCAAGTAATCCAGGATAGGCTT174140.15319924162240584No Hit
CGCGACCTCAGATCAGACGTGG170570.1500585428019626No Hit
AGCAGCATTGTACAGGGCTATGT169260.14890607348689797No Hit
CGCGACCTCAGATCAGACGTGGCG167400.14726974300901996No Hit
TCGTTTCCCGGCCAATGCACCA166220.14623164087789306No Hit
TATTGCACTTGTCCCGGCCTGTT164220.14447214574038983No Hit
AGCTCGGTCTGAGGCCCCTCAGT161890.14242233390519857No Hit
CCTCGACACAAGGGTTTGT161830.1423695490510735No Hit
AGCAGCATTGTACAGGGCTATG159690.140486889253945No Hit
TAGCAGCACGTAAATATTGGC158450.13939600226869303No Hit
CCCCCCACTGCTAAATTTGACTGG151400.13319378190899414No Hit
TACCTGGTTGATCCTGCC149690.13168941356642888No Hit
TGAGGTAGTAGGTTGTGTGGTT149530.13154865395542864No Hit
TGATGTAGTAGATTGTATAGTT144470.1270971312575455No Hit
AACGGAATCCCAAAAGCAGCTG142290.12517928155766697No Hit
CCCCCACTGCTAAATTTGACTGGCTT141650.12461624311366594No Hit
ACTGGACTTGGAGTCAGAAGG137600.12105326546022191No Hit
CAAAGTGCTGTTCGTGCAGGTAG137080.12059579672447107No Hit
TTCAAGTAATCCAGGATAGGC135770.11944332740940644No Hit
TGAGGTAGTAGGTTGTATAGT135050.1188099091599053No Hit
TGTAAACATCCTACACTCAGCT134760.11855478236496733No Hit
TTCACAGTGGCTAAGTTCCGC133290.11726155343890245No Hit
TTCACAGTGGCTAAGTTCTG130910.11516775422527363No Hit
ATCCCACCGCTGCCACCA127890.11251091656764374No Hit
CAAAGAATTCTCCTTTTGGGCT125830.11069863657601543No Hit
CAAAGAATTCTCCTTTTGGGCTT125800.11067224414895288No Hit
ACTGGACTTGGAGTCAGAAGGCA125560.11046110473245249No Hit
TATTGCACTTGTCCCGGCCTGTAG125250.1101883829861395No Hit
TCGTTCGCGCTTTCCCCTG125180.11012680065632686No Hit
CAAAGTGCTGTTCGTGCAGGT124220.10928224299032532No Hit
TTCACAGTGGCTAAGTTCTGC123940.10903591367107487No Hit
ACTTGACTTGGAGTCAGAAGGC122460.1077338872693225No Hit
TAGCAGCACGTAAATATTGGCGT121060.10650224067307024No Hit
TGTAAACATCCCCGACTGGAAGC119460.10509464456306765No Hit
CACTAGATTGTGAGCTCCTGGA115340.101470084579811No Hit
TGTCAGTTTGTCAAATACCCCA115170.10132052749312324No Hit
CCCCCCACTGCTAAATTTGACTGGCT113690.10001850109137085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)