FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11797079
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9878688.37383559099672No Hit
CCCCCCACTGCTAAATTTGACTG9755488.269402959834379No Hit
TAGCAGCACGTAAATATTGGCG4096933.472834249902031No Hit
AAGCTGCCAGTTGAAGAACTGT4096753.4726816697590985No Hit
TTCAAGTAATCCAGGATAGGCT3645793.0904175516668153No Hit
AACATTCAACGCTGTCGGTGAGT3537872.998937279304479No Hit
CCCCCACTGCTAAATTTGACTG3019272.5593369341681953No Hit
TAGCTTATCAGACTGATGTTGAC2972632.519801723799595No Hit
TAGCTTATCAGACTGATGTTGACT2160491.831377072239662No Hit
AACATTCAACGCTGTCGGTGAG1746231.4802223499562899No Hit
TATTGCACTTGTCCCGGCCTGTAA1698611.439856425476171No Hit
TAGCTTATCAGACTGATGTTGA1641701.3916156702858393No Hit
TGTAAACATCCTTGACTGGAAGCT1485881.2595321265543784No Hit
TGAGGTAGTAGGTTGTATAGTT1256921.0654501847448847No Hit
TATTGCACTTGTCCCGGCCTGTAT1245681.0559224024862426No Hit
AGCAGCATTGTACAGGGCTATGA1168600.9905841946129207No Hit
TTCAAGTAATTCAGGATAGGTT864660.7329441465976451No Hit
TATTGCACTTGTCCCGGCCTGT854950.7247132955539248No Hit
TGTAAACATCCCCGACTGGAAGCT819770.6948923542853277No Hit
CATAAAGTAGAAAGCACTACT806950.684025257438727No Hit
TGAGGTAGTAGATTGTATAGTT776880.658535896894477No Hit
CAACGGAATCCCAAAAGCAGCTGT698540.5921296280206313No Hit
CCCATAAAGTAGAAAGCACTAC607170.5146782521334307No Hit
TATTGCACTTGTCCCGGCCTGTA573420.4860694753336821No Hit
TTCTCACTACTGCACTTGACTAG555140.47057411415147765No Hit
ACTGGACTTGGAGTCAGAAGGC521930.4424230777805252No Hit
TGTAAACATCCTACACTCTCAGCT477500.4047612125001452No Hit
CCCCCACTGCTAAATTTGACTGGCTT471740.3998786479263214No Hit
TAGCTTATCAGACTGATGTTGACA436840.3702950535467297No Hit
CTTCTCACTACTGCACTTGACTAG433570.3675231809501318No Hit
GCCTGTCTGAGCGTCGCT431630.36587870607630923No Hit
TTAATGCTAATCGTGATAGGGGTT427990.36279319651924No Hit
AACATTCAACGCTGTCGGTGAGTT362390.30718621109513633No Hit
TGAGGTAGTAGTTTGTACAGTT346850.2940134587553411No Hit
TGATGTAGTAGGTTGTATAGTT345830.2931488379453931No Hit
CCCCCCACTGCTAAATTTGACTGGCT330870.2804677327328231No Hit
CAAAGAATTCTCCTTTTGGGCT320280.27149093432365756No Hit
CATTGCACTTGTCTCGGTCTGA319860.2711349139901496No Hit
CGCCTGTCTGAGCGTCGCT300530.2547495019741752No Hit
CCCCCCACTGCTAAATTTGACTGG297620.25228278966344125No Hit
CTCCCACTGCTTCACTTGACTAG281990.23903374725217996No Hit
CAAAGAATTCTCCTTTTGGGCTT255340.21644340942363785No Hit
CACCCGGCTGTGTGCACATGTGC246640.20906870251525822No Hit
TGAGGTAGTAGTTTGTGCTGTT241030.2043132880605445No Hit
ACCCCCCACTGCTAAATTTGACTG237410.20124473185268998No Hit
CCCATAAAGTAGAAAGCACT234970.19917642324850074No Hit
CTACGCCTGTCTGAGCGTCGCT227880.19316646095190174No Hit
AGCTCGGTCTGAGGCCCCTCAGT225530.19117444241917853No Hit
TGATGTAGTAGATTGTATAGTT214760.182045063867081No Hit
TGTAAACATCCCCGACTGGAAGC209630.17769652979351924No Hit
TAGCAGCACGTAAATATTGGC209590.1776626230950899No Hit
TTCAAGTAATCCAGGATAGGCTT205830.17447539344273275No Hit
GGAATACCGGGTGCTGTAGGCTTT201680.17095757348068957No Hit
TGTCAGTTTGTCAAATACCCCA187990.15935300594325089No Hit
CAAAGTGCTGTTCGTGCAGGTAG185400.15715754721995165No Hit
CTCCCACTGCTTCACTTGACTAGCCT180270.15280901314638987No Hit
TGAGAACTGAATTCCATAGGCTGT176820.14988456040686005No Hit
TTCACAGTGGCTAAGTTCCG174290.14773996173120482No Hit
TGTAAACATCCTACACTCAGCT174110.14758738158827284No Hit
AGCAGCATTGTACAGGGCTATGT171600.14545973626183228No Hit
AACGGAATCCCAAAAGCAGCTG166050.14075518185476252No Hit
ACTTGACTTGGAGTCAGAAGGC165490.14028048807675186No Hit
TGTCAGTTTGTCAAATACCCCAA157580.13357543846235156No Hit
TAGCAGCACGTAAATATTGGCGT152360.1291506143173238No Hit
ACTGGACTTGGAGTCAGAAGGCA151060.12804864661837054No Hit
TATTGCACTTGTCCCGGCCTGTT150250.12736203597517656No Hit
TTCAAGTAATCCAGGATAGGC149080.12637026504611862No Hit
TACGCCTGTCTGAGCGTCGCT148370.12576842114899797No Hit
TTATAAAGCAATGAGACTGATT147110.12470036014847405No Hit
CACTAGATTGTGAGCTCCTGGA146290.12400527283067274No Hit
CACTAGATTGTGAGCTCCTGGAA144150.1221912644647035No Hit
AGCAGCATTGTACAGGGCTATG142320.12064003301156159No Hit
CAACGGAATCCCAAAAGCAGCT141690.12010600251129962No Hit
AACCGGGCGGAAACACCA140340.11896165143930967No Hit
TCCTGTCTGAGCGTCGCT138550.11744432668459709No Hit
CCCCCACTGCTAAATTTGACTGGCT134990.11442663052438659No Hit
TTCACAGTGGCTAAGTTCCGC134650.11413842358773726No Hit
CGCGACCTCAGATCAGACGTGGCG133490.11315512933328667No Hit
ATCACATTGCCAGGGATTTCCA132690.11247699536470002No Hit
TATTGCACTTGTCCCGGCCTGTAAT132260.11211249835658471No Hit
GGAATACCGGGTGCTGTAGGCTT131740.11167171127700339No Hit
TTCACAGTGGCTAAGTTCTGC127180.1078063476560596No Hit
ACCGGGTGCTGTAGGCTT124360.10541592541679172No Hit
TATTGCACTTGTCCCGGCCTGTAG123660.10482255819427844No Hit
GGGCTACGCCTGTCTGAGCGTCGCT122540.10387317063825716No Hit
ATCACATTGCCAGGGATTTCC122030.10344086023328317No Hit
AACATTCAACGCTGTCGGTGAGA121010.10257623942333521No Hit
TATTGCACTTGTCCCGGCCTGTAAA120420.10207611562150258No Hit
TGAGGTAGTAGGTTGTGTGGTT119950.10167771191495793No Hit
TAGCAGCACATCATGGTTTACA119200.10104196131940797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)