FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8800161
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG142689016.214362441778054No Hit
CAACGGAATCCCAAAAGCAGCTG7583368.617296888091024No Hit
CCCCCACTGCTAAATTTGACTG4763715.413207781084915No Hit
TATTGCACTTGTCCCGGCCTGTAA2335132.653508271041859No Hit
AACATTCAACGCTGTCGGTGAGT2088762.373547483960805No Hit
TTCAAGTAATCCAGGATAGGCT1898182.1569832642834603No Hit
TAGCTTATCAGACTGATGTTGACT1795462.0402581270956293No Hit
AACATTCAACGCTGTCGGTGAG1727131.962611820397377No Hit
TATTGCACTTGTCCCGGCCTGTAT1670811.898612991285046No Hit
TAGCTTATCAGACTGATGTTGAC1665181.8922153810595057No Hit
TAGCAGCACGTAAATATTGGCG1565601.7790583604095425No Hit
AAGCTGCCAGTTGAAGAACTGT1245311.4150991101185535No Hit
TAGCTTATCAGACTGATGTTGA1021311.1605583125126915No Hit
TGAGGTAGTAGGTTGTATAGTT871650.990493242112275No Hit
TATTGCACTTGTCCCGGCCTGTA781980.8885973790706783No Hit
TATTGCACTTGTCCCGGCCTGT709510.8062466129881032No Hit
AGCAGCATTGTACAGGGCTATGA599970.681771617587451No Hit
TGTAAACATCCTTGACTGGAAGCT562060.6386928602783517No Hit
TTAATGCTAATCGTGATAGGGGTT513840.5838984082223041No Hit
TGTAAACATCCCCGACTGGAAGCT483320.5492172245485054No Hit
CCCCCACTGCTAAATTTGACTGGCTT442100.5023771724176409No Hit
TGAGGTAGTAGATTGTATAGTT434660.49392278164001774No Hit
CACCCGGCTGTGTGCACATGTGC395420.4493326883451337No Hit
CAACGGAATCCCAAAAGCAGCTGT393200.44681000722600417No Hit
ACTGGACTTGGAGTCAGAAGGC387860.4407419364259358No Hit
TTCAAGTAATTCAGGATAGGTT352830.40093584651462627No Hit
GGAATACCGGGTGCTGTAGGCTTT346850.3941405162928269No Hit
GCCTGTCTGAGCGTCGCT335610.3813680226986756No Hit
CAACGGAATCCCAAAAGCAGCT302200.34340280819862273No Hit
CATTGCACTTGTCTCGGTCTGA295000.3352211397041486No Hit
TAGCTTATCAGACTGATGTTGACA289490.32895989062018294No Hit
ACCCCCCACTGCTAAATTTGACTG286890.32600539921940064No Hit
CATAAAGTAGAAAGCACTACT270540.3074261936798656No Hit
CACTAGATTGTGAGCTCCTGGAA258300.2935173572392596No Hit
TATTGCACTTGTCCCGGCCTGTAG256880.2919037503972939No Hit
TTCTCACTACTGCACTTGACTAG249670.2837107184743552No Hit
TGAGAACTGAATTCCATAGGCTGT239930.272642739149886No Hit
CACTAGATTGTGAGCTCCTGGA236500.2687450831865462No Hit
TGATGTAGTAGGTTGTATAGTT224060.2546089781766493No Hit
CAAAGAATTCTCCTTTTGGGCT221660.2518817553451579No Hit
TATTGCACTTGTCCCGGCCTGTAAT221080.25122267649421415No Hit
CTTCTCACTACTGCACTTGACTAG217830.24752956224323625No Hit
TATTGCACTTGTCCCGGCCTGTAAA212900.24192739201021438No Hit
GGAATACCGGGTGCTGTAGGCTT203440.23117758868275248No Hit
CCCCCCACTGCTAAATTTGACTGG197930.2249163395987869No Hit
CCCATAAAGTAGAAAGCACTAC192540.21879145165639582No Hit
CAACACCAGTCGATGGGCTGT189100.21488243226459153No Hit
AACATTCAACGCTGTCGGTGAGTT186550.21198475800613195No Hit
CCCCCCACTGCTAAATTTGACTGGCT167960.19086014449053829No Hit
TATTGCACTTGTCCCGGCCTGTT154200.17522406692332107No Hit
CTCCCACTGCTTCACTTGACTAG153830.1748036200701328No Hit
ACTGGACTTGGAGTCAGAAGGCA139520.15854255393736547No Hit
CAAAGAATTCTCCTTTTGGGCTT135290.15373582369686192No Hit
AACGGAATCCCAAAAGCAGCTG135220.1536562796976101No Hit
TGAGGTAGTAGTTTGTACAGTT134980.15338355741446094No Hit
CTCCCACTGCTTCACTTGACTAGCCT134480.15281538599123357No Hit
CCCATAAAGTAGAAAGCACT133640.1518608580002116No Hit
TGAGAACTGAATTCCATGGGTT131000.14886091288557107No Hit
ATGCACCTGGGCAAGGATTCTGA128550.14607687291175697No Hit
TAGCAGCACGTAAATATTGGC128130.14559960891624596No Hit
CCCGCCACTGCTAAATTTGACTG127800.1452246157769159No Hit
TGTAAACATCCTACACTCTCAGCT126590.14384964093270566No Hit
CACCCGTAGAACCGACCTTGCG126210.14341783065105287No Hit
ACCGGGTGCTGTAGGCTT123450.14028152439483776No Hit
AGCTCGGTCTGAGGCCCCTCAGT122220.13888382269369845No Hit
CCCCCCACTGCTAAATTTGACTGTA117930.1340089118824076No Hit
CAACGGAATCCCAAAAGCAGCTGA117250.13323619874681839No Hit
CTCCCCACTGCTAAATTTGACTG116240.13208849247189908No Hit
TGAGGTAGTAGTTTGTGCTGTT114710.13034988791682334No Hit
GGGAATACCGGGTGCTGTAGGCTT114370.12996353134902872No Hit
CCCCCCACTGCTAAATTTGACTGT112050.12732721594525373No Hit
TATTGCACTTGTCCCGGCCTGTTT109590.12453181254297506No Hit
ACCGGGTGCTGTAGGCTTT108970.12382727997817312No Hit
TGATGTAGTAGATTGTATAGTT108890.12373637255045675No Hit
ACCGGGTGCTGTAGGCTTA108820.12365682855120491No Hit
TCGTACGACTCTTAGCGG106580.12111142057514629No Hit
ACTTGACTTGGAGTCAGAAGGC106360.12086142514892625No Hit
AGCTACATCTGGCTACTGGGTCTC106360.12086142514892625No Hit
AGCAGCATTGTACAGGGCTATGT105280.11963417487475513No Hit
GGGAATACCGGGTGCTGTAGGCTTT103900.11806602174664758No Hit
TCGTTTCCCGGCCAATGCACCA102450.11641832461928821No Hit
CGCGACCTCAGATCAGACGTGGCG102380.11633878062003637No Hit
ACTGGACTTGGAGTCAGAAGGCT101220.11502062291814888No Hit
TTATAAAGCAATGAGACTGATT100760.1144979052087797No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC100490.11419109264023691No Hit
GAATACCGGGTGCTGTAGGCTTT95020.1079752972701295No Hit
CGCCCCACTGCTAAATTTGACTG93930.10673668356749383No Hit
GACCCGCGGGCGCTCTCCAGTCCT93610.1063730538566283No Hit
TTCAAGTAATCCAGGATAGGCTT91130.10355492359742054No Hit
TGTAAACATCCCCGACTGGAAGC88610.10069133962435459No Hit
CAAAGTGCTGTTCGTGCAGGTAG88420.10047543448352819No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)