FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7165256
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6917519.654239848513438No Hit
CAACGGAATCCCAAAAGCAGCTG4841946.757525481294737No Hit
TAGCAGCACGTAAATATTGGCG2211043.0857794892464416No Hit
AACATTCAACGCTGTCGGTGAGT2101082.9323167239244485No Hit
TTCAAGTAATCCAGGATAGGCT1955802.7295605348922636No Hit
CCCCCACTGCTAAATTTGACTG1923052.6838538637000546No Hit
TAGCTTATCAGACTGATGTTGAC1717452.3969136622613343No Hit
AAGCTGCCAGTTGAAGAACTGT1681292.3464479147709447No Hit
TAGCTTATCAGACTGATGTTGACT1490702.080456022785508No Hit
TATTGCACTTGTCCCGGCCTGTAA1216131.697259665251318No Hit
AACATTCAACGCTGTCGGTGAG1042931.4555376667630577No Hit
TAGCTTATCAGACTGATGTTGA933771.3031914002793479No Hit
TATTGCACTTGTCCCGGCCTGTAT896491.2511625544153622No Hit
TGTAAACATCCTTGACTGGAAGCT776571.0837993785567466No Hit
AGCAGCATTGTACAGGGCTATGA526270.734474804528966No Hit
TGAGGTAGTAGGTTGTATAGTT512840.7157315802812907No Hit
TATTGCACTTGTCCCGGCCTGT507660.7085022503034085No Hit
TGTAAACATCCCCGACTGGAAGCT467060.6518399342605484No Hit
TATTGCACTTGTCCCGGCCTGTA431330.6019743048957358No Hit
CATAAAGTAGAAAGCACTACT396940.5539788110850471No Hit
ACTGGACTTGGAGTCAGAAGGC383060.5346075562408378No Hit
CCCCCACTGCTAAATTTGACTGGCTT364780.5090955577860722No Hit
CAACGGAATCCCAAAAGCAGCTGT350130.4886496728100155No Hit
CCCATAAAGTAGAAAGCACTAC339690.4740793629704228No Hit
TTCAAGTAATTCAGGATAGGTT331130.4621328254007952No Hit
TGAGGTAGTAGATTGTATAGTT290500.4054286406514994No Hit
CTTCTCACTACTGCACTTGACTAG275730.3848152808497003No Hit
TTCTCACTACTGCACTTGACTAG271370.37873036218105816No Hit
TGTAAACATCCTACACTCTCAGCT270420.37740451981059714No Hit
TAGCTTATCAGACTGATGTTGACA264540.3691982533492174No Hit
CCCCCCACTGCTAAATTTGACTGGCT254210.35478146209988864No Hit
AACATTCAACGCTGTCGGTGAGTT248600.34695201399642944No Hit
CAAAGAATTCTCCTTTTGGGCT240540.33570328820072864No Hit
GGAATACCGGGTGCTGTAGGCTTT232850.32497094311773367No Hit
CATTGCACTTGTCTCGGTCTGA184710.25778562552405665No Hit
CCCCCCACTGCTAAATTTGACTGG175580.24504358253215236No Hit
CAAAGAATTCTCCTTTTGGGCTT171610.23950295704717317No Hit
GTCAGGATGGCCGAGCGGTCTAAGG158140.22070390785758387No Hit
CTCCCACTGCTTCACTTGACTAG157620.21997818361269997No Hit
ACCCCCCACTGCTAAATTTGACTG154130.21510745743069057No Hit
GGAATACCGGGTGCTGTAGGCTT141800.19789941908565445No Hit
TGTCAGTTTGTCAAATACCCCAA139360.19449409762889142No Hit
CACCCGGCTGTGTGCACATGTGC139010.1940056293871426No Hit
CGCCTGTCTGAGCGTCGCT137330.1916609818267484No Hit
CAACGGAATCCCAAAAGCAGCT136430.19040492063368009No Hit
CACTAGATTGTGAGCTCCTGGAA136060.1898885399209742No Hit
TGAATACCGGGTGCTGTAGGCTTT134110.1871670740026595No Hit
CTACGCCTGTCTGAGCGTCGCT133880.18684608058665314No Hit
TATTGCACTTGTCCCGGCCTGTAG130240.1817660108724657No Hit
CCCATAAAGTAGAAAGCACT128340.17911432613154366No Hit
ACTGGACTTGGAGTCAGAAGGCA121030.16891231799673312No Hit
CACTAGATTGTGAGCTCCTGGA120280.16786560033584286No Hit
CTCCCACTGCTTCACTTGACTAGCCT116850.16307861156670467No Hit
TGAGGTAGTAGTTTGTACAGTT116460.16253431838304172No Hit
TGTAAACATCCCCGACTGGAAGC116270.1622691499089495No Hit
TTCAAGTAATCCAGGATAGGCTT111950.15624005618222156No Hit
TATTGCACTTGTCCCGGCCTGTT109270.15249978507397363No Hit
TAGCAGCACGTAAATATTGGC108990.1521090104805746No Hit
TCGTTTCCCGGCCAATGCACCA106550.14870368902381156No Hit
TGATGTAGTAGGTTGTATAGTT106390.14848038925615498No Hit
TTCACAGTGGCTAAGTTCCG102460.14299558871308996No Hit
CGCCCCACTGCTAAATTTGACTG102000.14235360188107724No Hit
ATCACATTGCCAGGGATTTCCA98210.13706418863471173No Hit
TATTGCACTTGTCCCGGCCTGTAAT98020.1367990201606195No Hit
TATTGCACTTGTCCCGGCCTGTAAA97260.13573834626425071No Hit
AGCAGCATTGTACAGGGCTATGT96540.13473349730979606No Hit
TGTCAGTTTGTCAAATACCCCA95480.13325413634907113No Hit
TACGCCTGTCTGAGCGTCGCT94360.131691037975475No Hit
CCCCCACTGCTAAATTTGACTGGCT92410.12896957205716028No Hit
AGCTCGGTCTGAGGCCCCTCAGT92110.12855088499280418No Hit
TCGTACGACTCTTAGCGG91700.12797867933818416No Hit
TGTAAACATCCTACACTCAGCT90720.1266109682612875No Hit
TGAGGTAGTAGTTTGTGCTGTT90370.12612250001953873No Hit
TTTAAACATCCTTGACTGGAAGCT90340.12608063131310313No Hit
CAAAGTGCTGTTCGTGCAGGTAG88310.12324751551096012No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC86600.12086099924413028No Hit
AACGGAATCCCAAAAGCAGCTG86490.12070748065386638No Hit
TAGCAGCACGTAAATATTGGCGT85950.11995384393802537No Hit
ACTTGACTTGGAGTCAGAAGGC85300.11904668863192047No Hit
CAACACCAGTCGATGGGCTGT84960.11857217662565023No Hit
TTCAAGTAATCCAGGATAGGC83020.11586466694281404No Hit
TTCAGGATGGCCGAGCGGTCTAAGG82780.11552971729132916No Hit
CGCGACCTCAGATCAGACGTGGCG81830.11420387492086814No Hit
TGAATACCGGGTGCTGTAGGCTT80810.11278033890205737No Hit
ACTGGACTTGGAGTCAGAAGG79920.11153823394446757No Hit
ATGCACCTGGGCAAGGATTCTGA79740.1112870217058539No Hit
TTATAAAGCAATGAGACTGATT75410.1052439717436474No Hit
TATTGCACTTGTCCCGGCCTGTTT73420.10246668088341854No Hit
CTCCCCACTGCTAAATTTGACTG72940.10179678158044876No Hit
TGCAAGTAATCCAGGATAGGCT72840.10165721922566338No Hit
ACTGGACTTGGAGTCAGAAGGCT72740.10151765687087802No Hit
GGGCTACGCCTGTCTGAGCGTCGCT72170.10072215144860142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)