FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17579592
Sequences flagged as poor quality0
Sequence length18-26
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT1009974957.45155518967676No Hit
CAACGGAATCCCAAAAGCAGCTG6328913.6001461239828543No Hit
TTCAAGTAATCCAGGATAGGCT2903561.651665180852889No Hit
CCCCCCACTGCTAAATTTGACTG2609591.4844428698914058No Hit
TAGCAGCACGTAAATATTGGCG2590381.473515426296583No Hit
AAGCTGCCAGTTGAAGAACTGT2411841.3719544799446994No Hit
AACATTCAACGCTGTCGGTGAGT1801241.0246199115428845No Hit
CCCCCACTGCTAAATTTGACTG1743100.9915474716364293No Hit
AACATTCAACGCTGTCGGTGAG1540210.8761352368132321No Hit
CATAAAGTAGAAAGCACTACT1398790.7956896838106366No Hit
TGAGGTAGTAGGTTGTATAGTT1202550.6840602444015765No Hit
GCCGGTCTGAGCGTCGCT1075260.6116524206022529No Hit
CGCCTGTCTGAGCGTCGCT1024920.5830169437379434No Hit
TAGCTTATCAGACTGATGTTGAC934140.5313775200243555No Hit
AACCGGGCGGAAACACCA922060.5245059157231863No Hit
TCGTACGACTCTTAGCGG909690.5174693474114758No Hit
TAGCTTATCAGACTGATGTTGA851110.4841466172821303No Hit
TATTGCACTTGTCCCGGCCTGT750850.4271145769480884No Hit
TAGCTTATCAGACTGATGTTGACT750350.4268301562402586No Hit
GCCTGTCTGAGCGTCGCG679460.3865049882841422No Hit
TATTGCACTTGTCCCGGCCTGTAA653400.3716809809920503No Hit
GCCTGTCTGAGCGTCGCA626880.356595306648755No Hit
TGAGGTAGTAGATTGTATAGTT582700.3314638929049093No Hit
TATTGCACTTGTCCCGGCCTGTAT559010.3179880397679309No Hit
TCCTGTCTGAGCGTCGCT547630.3115146244577235No Hit
CAACACCAGTCGATGGGCTGT508440.2892217293780197No Hit
GCCTGTCTGAGCGTCGCTT490430.27897689548198845No Hit
TTCAAGTAATTCAGGATAGGTT482300.2743522147726751No Hit
TGTAAACATCCTTGACTGGAAGCT455560.25914139531793456No Hit
CATTGCACTTGTCTCGGTCTGA422030.2400681426508647No Hit
CCCATAAAGTAGAAAGCACT418480.23804875562527275No Hit
NCCTGTCTGAGCGTCGCT366930.20872498064801503No Hit
TATTGCACTTGTCCCGGCCTGTA364640.20742233380615432No Hit
AGCAGCATTGTACAGGGCTATGA348650.19832655956975564No Hit
CCCATAAAGTAGAAAGCACTAC339670.19321836365713152No Hit
CACCCGGCTGTGTGCACATGTGC319270.18161399877767356No Hit
CAACGGAATCCCAAAAGCAGCTGT300440.17090271492080134No Hit
TGATGTAGTAGGTTGTATAGTT276310.1571765715609327No Hit
TGAGGTAGTAGTTTGTACAGTT255220.14517970610466957No Hit
ATCCCGGACGAGCCCCCA255020.14506593782153762No Hit
ACCGGGTGCTGTAGGCTT247380.14071998940589747No Hit
ACTGGACTTGGAGTCAGAAGGC241910.1376084268622389No Hit
TGTAAACATCCCCGACTGGAAGCT233070.1325798687478071No Hit
CTACGCCTGTCTGAGCGTCGCT216640.12323380428851818No Hit
TTCACAGTGGCTAAGTTCCGC204630.11640201888644514No Hit
TAGCAGCACGTAAATATTGGC194480.11062827851749915No Hit
TTCTCACTACTGCACTTGACTAG191570.1089729499979294No Hit
ACGCCTGTCTGAGCGTCGCT183100.10415486320729173No Hit
AACGGAATCCCAAAAGCAGCTG177240.10082145251152587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)