FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11128162
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10385429.33255644552982No Hit
CCCCCCACTGCTAAATTTGACTG5494244.9372394111444455No Hit
TTCAAGTAATCCAGGATAGGCT5157194.634359205051113No Hit
TAGCAGCACGTAAATATTGGCG5119534.600517138409739No Hit
AAGCTGCCAGTTGAAGAACTGT4288653.853870926753223No Hit
GCCTGTCTGAGCGTCGCT4236263.806792172867361No Hit
CCCCCACTGCTAAATTTGACTG2611102.346389277941856No Hit
AACATTCAACGCTGTCGGTGAGT2207671.9838586102538767No Hit
CATAAAGTAGAAAGCACTACT2186971.9652571556740457No Hit
AACATTCAACGCTGTCGGTGAG1990311.7885343509557103No Hit
TAGCTTATCAGACTGATGTTGAC1969161.7695285169284918No Hit
TGAGGTAGTAGGTTGTATAGTT1535621.379940371105309No Hit
TAGCTTATCAGACTGATGTTGACT1459351.3114025478780773No Hit
TAGCTTATCAGACTGATGTTGA1349141.2123655281078762No Hit
TATTGCACTTGTCCCGGCCTGTAA1306461.1740123840756451No Hit
TGTAAACATCCTTGACTGGAAGCT1281531.1516097626903705No Hit
TATTGCACTTGTCCCGGCCTGTAT1213841.0907821075933295No Hit
TATTGCACTTGTCCCGGCCTGT984630.8848091895139557No Hit
TGAGGTAGTAGATTGTATAGTT983990.884234072077671No Hit
TTCAAGTAATTCAGGATAGGTT920430.8271177216866541No Hit
TATTGCACTTGTCCCGGCCTGTA646770.5812011004153247No Hit
AGCAGCATTGTACAGGGCTATGA631520.5674971302538551No Hit
CAACGGAATCCCAAAAGCAGCTGT623280.5600924932616905No Hit
CCCATAAAGTAGAAAGCACTAC564610.5073703995322858No Hit
CATTGCACTTGTCTCGGTCTGA516500.4641377435015774No Hit
TGTAAACATCCCCGACTGGAAGCT512880.46088473550259246No Hit
AACCGGGCGGAAACACCA499660.4490049659593381No Hit
TTCTCACTACTGCACTTGACTAG470750.4230258330171685No Hit
TGAGGTAGTAGTTTGTACAGTT437690.39331742294909083No Hit
TCGTACGACTCTTAGCGG404060.36309679891432206No Hit
CACCCGGCTGTGTGCACATGTGC403880.362935047135367No Hit
ACTGGACTTGGAGTCAGAAGGC395000.3549552927069178No Hit
TAGCAGCACGTAAATATTGGC387190.347937062742257No Hit
TGTCAGTTTGTCAAATACCCCAA383260.3444054822350717No Hit
TGTAAACATCCTACACTCTCAGCT376960.3387441699716449No Hit
CCCATAAAGTAGAAAGCACT368710.3313305467695384No Hit
TGTCAGTTTGTCAAATACCCCA341700.30705879371633876No Hit
CTTCTCACTACTGCACTTGACTAG332060.2983960873323016No Hit
CAACACCAGTCGATGGGCTGT310090.2786533840898434No Hit
CTCCCACTGCTTCACTTGACTAG301220.2706826158713362No Hit
CCCCCACTGCTAAATTTGACTGGCTT293820.26403282051429516No Hit
TTCACAGTGGCTAAGTTCCGC286370.25733809410754444No Hit
TGATGTAGTAGGTTGTATAGTT269400.2420884958360599No Hit
CAAAGAATTCTCCTTTTGGGCT260030.2336684171204553No Hit
CAACGGAATCCCAAAAGCAGCT256720.23069398162967072No Hit
TGAGAACTGAATTCCATGGGTT255850.22991218136472133No Hit
AACGGAATCCCAAAAGCAGCTG253480.22778244960847982No Hit
TTCAAGTAATCCAGGATAGGC249050.22380155860419718No Hit
TGAGGTAGTAGTTTGTGCTGTT238650.21445590026457198No Hit
TTAATGCTAATCGTGATAGGGGTT234520.21074459555854777No Hit
TCCTGTCTGAGCGTCGCT234410.21064574724918633No Hit
CGCCTGTCTGAGCGTCGCT230690.20730287715078197No Hit
TTCACAGTGGCTAAGTTCTGC222710.20013188161710802No Hit
TAGCTTATCAGACTGATGTTGACA215220.1934012103705895No Hit
TTATAAAGCAATGAGACTGATT214350.1926194101056401No Hit
TTCACAGTGGCTAAGTTCCG214190.19247563074656893No Hit
CTACGCCTGTCTGAGCGTCGCT210200.18889013297973195No Hit
TAGCAGCACGTAAATATTGGCGT206450.18552030425150173No Hit
TGAGAACTGAATTCCATAGGCTGT206430.18550233183161785No Hit
TGTAAACATCCTACACTCAGCT205200.18439702800875832No Hit
TTCAAGTAATCCAGGATAGGCTT187450.1684465053618019No Hit
CCCCCCACTGCTAAATTTGACTGGCT186320.16743106363836183No Hit
GGAATACCGGGTGCTGTAGGCTTT186200.16732322911905848No Hit
CAAAGAATTCTCCTTTTGGGCTT184130.16546308366107537No Hit
TCAGTGCACTACAGAACTTTGT182850.16431284878850613No Hit
TGTCAGTTTGTCAAATACCCC178070.16001744043625535No Hit
TGTAAACATCCCCGACTGGAAGC176030.15818425360809807No Hit
TGATGTAGTAGATTGTATAGTT173800.15618032879104385No Hit
ATCACATTGCCAGGGATTTCC172420.1549402318190551No Hit
TATTGCACTTGTCCCGGCCTGTT165050.14831739509183994No Hit
TAGCAGCACATCATGGTTTACA155890.14008602678501625No Hit
CCCCCCACTGCTAAATTTGACTGG154000.13838763310598823No Hit
CCCATAAAGTAGAAAGCACTA153250.13771366736034216No Hit
AACATTCAACGCTGTCGGTGAGTT151700.13632080481934034No Hit
TATTGCACTTGTCCCGGCCTGTAG150620.13535029414561003No Hit
AGCTCGGTCTGAGGCCCCTCAGT149550.13438876968182167No Hit
GGAATACCGGGTGCTGTAGGCTT143310.12878137467804657No Hit
CTCCCACTGCTTCACTTGACTAGCCT141920.1275322914961159No Hit
ATCCCGGACGAGCCCCCA141000.12670556018145673No Hit
CACTAGATTGTGAGCTCCTGGA140620.12636408420366274No Hit
TGAGGTAGTAGGTTGTATAGT139180.12507006997202233No Hit
AGAGGTAGTAGGTTGCATAGTT131330.11801589516759373No Hit
AGCAGCATTGTACAGGGCTATGT129850.11668593609618552No Hit
TGTAAACATCCCCGACTGGA129160.11606588761019114No Hit
ACCCCCCACTGCTAAATTTGACTG129160.11606588761019114No Hit
TTCACAGTGGCTAAGTTCTG128730.11567948058268743No Hit
TAGCAGCACATAATGGTTTGT124490.11186932756730177No Hit
TCAGTGCACTACAGAACTTTGTC124160.11157278263921751No Hit
CAAAGTGCTGTTCGTGCAGGT123700.11115941698188793No Hit
ATCCCACCGCTGCCACCA123260.11076402374444226No Hit
TCGTTTCCCGGCCAATGCACCA119910.10775364341388992No Hit
CACTAGATTGTGAGCTCCTGGAA118720.10668428443079819No Hit
TGAGGTAGTAGGTTGTGTGGTT117320.10542621503892556No Hit
CAAAGTGCTGTTCGTGCAGGTAG116830.10498589075177014No Hit
CATAAAGTAGAAAGCACTAC114720.10308980045401928No Hit
AGCAGCATTGTACAGGGCTATG112650.10122965499603617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)