FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9488989
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8924139.404721619974477No Hit
CCCCCCACTGCTAAATTTGACTG5873596.189900736527358No Hit
TAGCAGCACGTAAATATTGGCG4308264.540272941616857No Hit
TTCAAGTAATCCAGGATAGGCT4247194.475914135847349No Hit
AAGCTGCCAGTTGAAGAACTGT3108863.276281593328857No Hit
GCCTGTCTGAGCGTCGCT2952613.1116170542509853No Hit
CCCCCACTGCTAAATTTGACTG2687962.832714844542448No Hit
AACATTCAACGCTGTCGGTGAGT2261002.382761746272443No Hit
TAGCTTATCAGACTGATGTTGAC2004032.1119531279886616No Hit
AACATTCAACGCTGTCGGTGAG1861371.961610451861626No Hit
TAGCTTATCAGACTGATGTTGACT1582131.6673325261521539No Hit
TATTGCACTTGTCCCGGCCTGTAA1475061.55449648007812No Hit
TGAGGTAGTAGGTTGTATAGTT1294521.364233850413358No Hit
TATTGCACTTGTCCCGGCCTGTAT1280461.3494166765289748No Hit
TGTAAACATCCTTGACTGGAAGCT1240471.3072730930555405No Hit
TAGCTTATCAGACTGATGTTGA1213681.279040369843405No Hit
CATAAAGTAGAAAGCACTACT1206541.2715158590657023No Hit
TTCAAGTAATTCAGGATAGGTT916100.9654347791951281No Hit
TGAGGTAGTAGATTGTATAGTT893650.9417757782204195No Hit
TATTGCACTTGTCCCGGCCTGT840770.886048028931217No Hit
CAACGGAATCCCAAAAGCAGCT675220.7115826564874298No Hit
AGCAGCATTGTACAGGGCTATGA592860.624787319281327No Hit
TATTGCACTTGTCCCGGCCTGTA575240.6062184285385935No Hit
TGTAAACATCCCCGACTGGAAGCT548840.5783967080159962No Hit
CAACGGAATCCCAAAAGCAGCTGT541990.5711778146228222No Hit
CCCATAAAGTAGAAAGCACTAC428860.4519554190651923No Hit
CACCCGGCTGTGTGCACATGTGC413560.435831467398687No Hit
TTCTCACTACTGCACTTGACTAG406960.4288760372680377No Hit
TGTAAACATCCTACACTCTCAGCT400100.4216466053443628No Hit
ACTGGACTTGGAGTCAGAAGGC396190.41752603991847814No Hit
CATTGCACTTGTCTCGGTCTGA388660.40959052645123734No Hit
TGAGGTAGTAGTTTGTACAGTT378450.3988306868097328No Hit
AACCGGGCGGAAACACCA348190.36694109351375576No Hit
CCCCCACTGCTAAATTTGACTGGCTT344350.362894297801378No Hit
TTAATGCTAATCGTGATAGGGGTT341030.3593955056750514No Hit
CCCATAAAGTAGAAAGCACT313390.3302670073703321No Hit
CAAAGAATTCTCCTTTTGGGCT311540.3283173792276501No Hit
CTTCTCACTACTGCACTTGACTAG300130.31629291592602754No Hit
TAGCAGCACGTAAATATTGGC296290.3122461202136497No Hit
TGAGAACTGAATTCCATAGGCTGT288020.3035307554893361No Hit
TAGCTTATCAGACTGATGTTGACA265590.2798928315756294No Hit
TGAGAACTGAATTCCATGGGTT250380.263863726683633No Hit
AACGGAATCCCAAAAGCAGCTG236810.24956294079379795No Hit
CTCCCACTGCTTCACTTGACTAG236320.24904655279924973No Hit
CCCCCCACTGCTAAATTTGACTGGCT214050.22557724537355878No Hit
CAAAGAATTCTCCTTTTGGGCTT212760.22421777493893186No Hit
TGTCAGTTTGTCAAATACCCCA206390.21750473100980516No Hit
TCGTTTCCCGGCCAATGCACCA204500.21551294874511923No Hit
TGAGGTAGTAGTTTGTGCTGTT201380.21222492722881228No Hit
TTCACAGTGGCTAAGTTCTGC196270.20683973814280954No Hit
TTCAAGTAATCCAGGATAGGCTT190210.20045338865921333No Hit
TCAGTGCACTACAGAACTTTGT182450.19227548899044988No Hit
AGCTCGGTCTGAGGCCCCTCAGT179220.1888715436386321No Hit
CAACACCAGTCGATGGGCTGT178860.1884921565405967No Hit
CAACGGAATCCCAAAAGCAGCTGA171370.1805987971953598No Hit
TTCAAGTAATCCAGGATAGGC170910.18011402479231456No Hit
TGTAAACATCCCCGACTGGAAGC170800.17999810095680374No Hit
TATTGCACTTGTCCCGGCCTGTAG166490.1754559943108797No Hit
TGTCAGTTTGTCAAATACCCCAA165210.17410706240675375No Hit
GGAATACCGGGTGCTGTAGGCTTT164810.1736855211867144No Hit
TTCACAGTGGCTAAGTTCCGC161150.16982841902335433No Hit
TATTGCACTTGTCCCGGCCTGTT155170.163526377783766No Hit
CCCCCCACTGCTAAATTTGACTGG153340.16159782670208597No Hit
TTATAAAGCAATGAGACTGATT152870.1611025157685397No Hit
AACATTCAACGCTGTCGGTGAGTT152080.160269971858962No Hit
CACTAGATTGTGAGCTCCTGGA147190.1551166304439809No Hit
ACCCCCCACTGCTAAATTTGACTG145940.15379931413135794No Hit
TGTAAACATCCTACACTCAGCT144900.15270330695925563No Hit
CACTAGATTGTGAGCTCCTGGAA144330.15210261072069955No Hit
ATCACATTGCCAGGGATTTCC140970.14856166447236896No Hit
CTCCCACTGCTTCACTTGACTAGCCT139260.14675957575670073No Hit
CTACGCCTGTCTGAGCGTCGCT136950.14432517521097346No Hit
AATGCACCCGGGCAAGGATTCT134570.14181700495173932No Hit
TCGTACGACTCTTAGCGG132890.14004653182757404No Hit
TCAGTGCACTACAGAACTTTGTC131920.13902429436897862No Hit
TGTCAGTTTGTCAAATACCCC128850.13578896550517658No Hit
GGAATACCGGGTGCTGTAGGCTT124890.131615707426787No Hit
TTCACAGTGGCTAAGTTCCG121530.12807476117845643No Hit
TGATGTAGTAGGTTGTATAGTT119320.125745745937739No Hit
CGCCTGTCTGAGCGTCGCT117750.12409119664908454No Hit
TAGCAGCACGTAAATATTGGCGT116270.12253149413493893No Hit
TATTGCACTTGTCCCGGCCTGTAAT114600.12077155954127462No Hit
AGCAGCATTGTACAGGGCTATGT113890.12002332387570477No Hit
CCCATAAAGTAGAAAGCACTA113680.11980201473518412No Hit
TAGCAGCACATCATGGTTTACA111770.11778915540949621No Hit
TATTGCACTTGTCCCGGCCTGTTT108050.11386882206313022No Hit
TATTGCACTTGTCCCGGCCTGTAAA105060.11071780144333607No Hit
TGTAAACATCCTTGACTGGAAGC104920.1105702620163223No Hit
AGAGGTAGTAGGTTGCATAGTT104380.11000118136926917No Hit
TGAGGTAGTAGGTTGTGTGGTT103940.10953748602722586No Hit
ATGCACCTGGGCAAGGATTCTGA101530.10699770017648877No Hit
GAATACCGGGTGCTGTAGGCTTT101420.10688177634097795No Hit
TGAGGTAGTAGGTTGTATAGT100250.10564876827236283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)