FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6158891
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG65388210.616878915376162No Hit
CCCCCCACTGCTAAATTTGACTG5275988.566444835604333No Hit
CCCCCACTGCTAAATTTGACTG2333213.788360599335173No Hit
TTCAAGTAATCCAGGATAGGCT2166893.518311981816207No Hit
TAGCAGCACGTAAATATTGGCG2030013.296064177787852No Hit
AACATTCAACGCTGTCGGTGAGT1838792.9855862037499934No Hit
AAGCTGCCAGTTGAAGAACTGT1797622.9187397536342177No Hit
AACATTCAACGCTGTCGGTGAG1409882.2891783601950415No Hit
TAGCTTATCAGACTGATGTTGAC1187251.9277009448616642No Hit
TAGCTTATCAGACTGATGTTGACT811281.3172501348051135No Hit
TGAGGTAGTAGGTTGTATAGTT784041.2730213929748067No Hit
TATTGCACTTGTCCCGGCCTGTAA727681.1815114117135699No Hit
TGTAAACATCCTTGACTGGAAGCT661261.0736673209511258No Hit
TATTGCACTTGTCCCGGCCTGTAT587430.953791843369204No Hit
TAGCTTATCAGACTGATGTTGA520980.8458990425386649No Hit
CATAAAGTAGAAAGCACTACT489350.7945423940771156No Hit
TATTGCACTTGTCCCGGCCTGT428190.6952388019206704No Hit
AGCAGCATTGTACAGGGCTATGA401360.6516757643543294No Hit
TGAGGTAGTAGATTGTATAGTT379310.6158738643044664No Hit
CAACGGAATCCCAAAAGCAGCTGT360700.5856573853961695No Hit
TGTAAACATCCCCGACTGGAAGCT344130.5587531911183361No Hit
TATTGCACTTGTCCCGGCCTGTA328300.5330505118535139No Hit
TGTCAGTTTGTCAAATACCCCAA325690.5288127359292444No Hit
ACTGGACTTGGAGTCAGAAGGC311760.5061950276437754No Hit
TTCAAGTAATTCAGGATAGGTT309860.5031100566644222No Hit
CAACGGAATCCCAAAAGCAGCT291760.4737216489137411No Hit
CCCATAAAGTAGAAAGCACTAC262990.42700869361058674No Hit
CACCCGGCTGTGTGCACATGTGC259150.4207738048944201No Hit
CCCCCACTGCTAAATTTGACTGGCTT213670.346929341662322No Hit
CATTGCACTTGTCTCGGTCTGA207930.3376094819668021No Hit
TGTAAACATCCTACACTCTCAGCT202180.32827338558191727No Hit
TTAATGCTAATCGTGATAGGGGTT201070.32647111306240034No Hit
CGCCTGTCTGAGCGTCGCT200770.32598401238144986No Hit
CCCATAAAGTAGAAAGCACT195760.31784943100957624No Hit
TGTCAGTTTGTCAAATACCCCA183700.29826798363536555No Hit
TTCTCACTACTGCACTTGACTAG182320.2960273205029932No Hit
AACGGAATCCCAAAAGCAGCTG178690.29013340226349194No Hit
TAGCAGCACGTAAATATTGGC168250.27318229856641396No Hit
CTTCTCACTACTGCACTTGACTAG167450.27188336341721264No Hit
TGAGGTAGTAGTTTGTACAGTT162930.26454437982422485No Hit
CAAAGAATTCTCCTTTTGGGCT160220.2601442370063052No Hit
TCGTACGACTCTTAGCGG157230.25528946688616505No Hit
TAGCTTATCAGACTGATGTTGACA153430.24911952492745854No Hit
AACATTCAACGCTGTCGGTGAGTT147890.24012439901923902No Hit
CCCCCCACTGCTAAATTTGACTGGCT136280.22127360266645407No Hit
CAACACCAGTCGATGGGCTGT134920.21906541291281173No Hit
CCCCCCACTGCTAAATTTGACTGG134010.21758787418059516No Hit
CAAAGAATTCTCCTTTTGGGCTT122830.19943525547050597No Hit
CTCCCACTGCTTCACTTGACTAG114970.18667321762960248No Hit
TGAGGTAGTAGTTTGTGCTGTT110420.17928552396851966No Hit
ACCCCCCACTGCTAAATTTGACTG107270.17417096681853925No Hit
AACCGGGCGGAAACACCA102820.1669456400511066No Hit
CTACGCCTGTCTGAGCGTCGCT102190.16592272862111052No Hit
TTCAAGTAATCCAGGATAGGCTT99470.16150634911382583No Hit
TGTAAACATCCCCGACTGGAAGC96430.1565703955468606No Hit
TTCACAGTGGCTAAGTTCTGC95470.155011673367819No Hit
GCCTGTCTGAGCGTCGCT94570.15355037132496743No Hit
CTCCCACTGCTTCACTTGACTAGCCT90950.14767268977483122No Hit
TAGCAGCACGTAAATATTGGCGT90370.14673096179166023No Hit
CAACGGAATCCCAAAAGCAGCTGA90190.1464387013830899No Hit
AATGCACCCGGGCAAGGATTCT90070.1462438611107097No Hit
ATCACATTGCCAGGGATTTCC88850.1442629850081776No Hit
ACTGGACTTGGAGTCAGAAGGCA86140.13986284219025796No Hit
TTCAAGTAATCCAGGATAGGC85770.1392620846837523No Hit
TATTGCACTTGTCCCGGCCTGTAG84450.13711884168757005No Hit
TCGTTTCCCGGCCAATGCACCA83930.13627453384058916No Hit
TACGCCTGTCTGAGCGTCGCT82000.13314085279314086No Hit
CACTAGATTGTGAGCTCCTGGA81310.13202052122695465No Hit
CACTAGATTGTGAGCTCCTGGAA80610.13088395297140346No Hit
TTATAAAGCAATGAGACTGATT80340.13044556235854798No Hit
TGAGGTAGTAGGTTGTGTGGTT79230.1286432898390311No Hit
TGAGAACTGAATTCCATAGGCTGT78580.12758790503030495No Hit
TGTAAACATCCTACACTCAGCT78580.12758790503030495No Hit
TATTGCACTTGTCCCGGCCTGTT77900.12648381015348378No Hit
TCAGTGCACTACAGAACTTTGTC75840.12313905214429027No Hit
ACTGGACTTGGAGTCAGAAGGCT75650.12283055504635494No Hit
TCAGTGCACTACAGAACTTTGT74940.12167775010143872No Hit
TTCACAGTGGCTAAGTTCCGC73910.12000537109684195No Hit
TTCACAGTGGCTAAGTTCCG71180.11557275490019225No Hit
ACTGGACTTGGAGTCAGAAGG69970.11360811548702518No Hit
ATCACATTGCCAGGGATTTCCA68970.11198444655052346No Hit
AGCAGCATTGTACAGGGCTATGT68800.11170842283131817No Hit
ACCACAGGGTAGAACCACGGAC68370.11101024518862243No Hit
AGCTCGGTCTGAGGCCCCTCAGT68120.110604327954497No Hit
ATGCACCTGGGCAAGGATTCTGA65830.10688612608990807No Hit
TAGCAGCACATCATGGTTTACA65820.10686988940054305No Hit
AGAGGTAGTAGGTTGCATAGTT65260.1059606347961021No Hit
ACCGGGTGCTGTAGGCTT64530.10477535647245584No Hit
TGAGAACTGAATTCCATGGGTT63940.10381739179991982No Hit
CCCCCCACTGCTAAATTTGACTGTA61590.10000176979914079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)