FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6787345
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT5261267.7515729640971545No Hit
CAACGGAATCCCAAAAGCAGCTG4275186.298751573700762No Hit
TAGCAGCACGTAAATATTGGCG2898634.270638961184381No Hit
AAGCTGCCAGTTGAAGAACTGT2492813.672732121322844No Hit
CCCCCCACTGCTAAATTTGACTG2435533.5883397705582967No Hit
TTCAAGTAATCCAGGATAGGCT2347403.4584951847887497No Hit
TAGCTTATCAGACTGATGTTGAC1799312.650977665051651No Hit
AACATTCAACGCTGTCGGTGAGT1787682.6338428354533328No Hit
TAGCTTATCAGACTGATGTTGA1161411.7111403648996775No Hit
CCCCCACTGCTAAATTTGACTG1097621.61715663488448No Hit
AACATTCAACGCTGTCGGTGAG965211.4220729902487645No Hit
TAGCTTATCAGACTGATGTTGACT958831.4126731439170985No Hit
CATAAAGTAGAAAGCACTACT877911.2934512685004225No Hit
TGAGGTAGTAGGTTGTATAGTT820901.2094567168753023No Hit
TATTGCACTTGTCCCGGCCTGT799151.1774117861991693No Hit
TATTGCACTTGTCCCGGCCTGTAA676350.9964868442667936No Hit
TATTGCACTTGTCCCGGCCTGTAT665070.979867680219585No Hit
TGTAAACATCCTTGACTGGAAGCT648810.9559113320451517No Hit
TTCAAGTAATTCAGGATAGGTT605510.8921161367220909No Hit
TCGTACGACTCTTAGCGG584910.861765535713891No Hit
AGCAGCATTGTACAGGGCTATGA520370.7666768080891718No Hit
TGAGGTAGTAGATTGTATAGTT479150.7059461394698516No Hit
CATTGCACTTGTCTCGGTCTGA403540.5945476471285901No Hit
TATTGCACTTGTCCCGGCCTGTA393360.5795491462420136No Hit
ACTGGACTTGGAGTCAGAAGGC364090.5364247728677414No Hit
TGTAAACATCCCCGACTGGAAGCT324690.47837556511419416No Hit
TGTAAACATCCTACACTCTCAGCT303120.44659583386434604No Hit
CGCCTGTCTGAGCGTCGCT297240.4379326526056948No Hit
AACCGGGCGGAAACACCA270110.3979612057439249No Hit
CAACGGAATCCCAAAAGCAGCTGT233560.34411098890656067No Hit
CCCATAAAGTAGAAAGCACT230700.3398972646889174No Hit
CCCATAAAGTAGAAAGCACTAC212540.3131415892370286No Hit
CACCCGGCTGTGTGCACATGTGC198100.29186670192836817No Hit
CTTCTCACTACTGCACTTGACTAG192860.28414645196317556No Hit
TTCTCACTACTGCACTTGACTAG190460.2806104596127057No Hit
TGAGGTAGTAGTTTGTGCTGTT174520.2571255770850016No Hit
TCAGTGCACTACAGAACTTTGT173060.2549745150717991No Hit
TTATAAAGCAATGAGACTGATT163700.2411841449049665No Hit
AACATTCAACGCTGTCGGTGAGTT163160.2403885466261108No Hit
CAACGGAATCCCAAAAGCAGCT158550.23359649465291657No Hit
TGTAAACATCCTACACTCAGCT157610.2322115643156492No Hit
TGAGGTAGTAGTTTGTACAGTT156230.230178368714129No Hit
CTACGCCTGTCTGAGCGTCGCT155720.22942697033965417No Hit
TTAATGCTAATCGTGATAGGGGTT154700.22792417359070444No Hit
CTCCCACTGCTTCACTTGACTAG151850.2237251826745215No Hit
TAGCTTATCAGACTGATGTTGACA151480.2231800505204907No Hit
CAACACCAGTCGATGGGCTGT149850.22077852238246323No Hit
TTCAAGTAATCCAGGATAGGCTT146700.21613753242247152No Hit
CACTAGATTGTGAGCTCCTGGA139870.20607468752509264No Hit
CAAAGAATTCTCCTTTTGGGCT138440.203967825416271No Hit
TAGCAGCACGTAAATATTGGC132000.1944795792758435No Hit
CAAAGAATTCTCCTTTTGGGCTT129850.1913119194618809No Hit
TTCACAGTGGCTAAGTTCCG128840.1898238560143915No Hit
GCGTTCGCGCTTTCCCCTG125810.18535966567192327No Hit
TTCAAGTAATCCAGGATAGGC124090.1828255378207532No Hit
TGTCAGTTTGTCAAATACCCCA120520.17756574919942922No Hit
TGATGTAGTAGGTTGTATAGTT119830.17654915139866914No Hit
TATTGCACTTGTCCCGGCCTGTT119160.17556202020082964No Hit
GGGCTACGCCTGTCTGAGCGTCGCT118950.17525262087016352No Hit
ACCGGGTGCTGTAGGCTT116480.1716134954094716No Hit
TGTAAACATCCCCGACTGGAAGC115040.16949189999918968No Hit
AACGGAATCCCAAAAGCAGCTG114720.16902043435246036No Hit
TGTCAGTTTGTCAAATACCCCAA112740.1661032406633227No Hit
AGCAGCATTGTACAGGGCTATGT110150.16228731558510728No Hit
AGCAGCATTGTACAGGGCTATG109590.16146225070333098No Hit
TGTCAGTTTGTCAAATACCCC108230.15945852170473138No Hit
CCCCCACTGCTAAATTTGACTGGCTT106450.15683599404479956No Hit
AGCTCGGTCTGAGGCCCCTCAGT105970.15612879557470558No Hit
TACGCCTGTCTGAGCGTCGCT105770.15583412954549974No Hit
TAGCAGCACGTAAATATTGGCGT105000.15469966533305735No Hit
CCCCCCACTGCTAAATTTGACTGGCT103860.15302006896658413No Hit
TCCTGTCTGAGCGTCGCT102270.15067747403439785No Hit
ACCACAGGGTAGAACCACGGAC95970.1413954941144144No Hit
CACTAGATTGTGAGCTCCTGGAA95600.14085036196038364No Hit
ACTGGACTTGGAGTCAGAAGG90460.13327744500979397No Hit
ATCCCGGACGAGCCCCCA88710.130699117254243No Hit
AATGACACGATCACTCCCGTTG87240.12853332193958023No Hit
TTCACAGTGGCTAAGTTCTGC86350.1272220581096143No Hit
CAAAGTGCTGTTCGTGCAGGTAG86100.126853725573107No Hit
CAAAGTGCTGTTCGTGCAGGT85410.1258371277723469No Hit
TATTGCACTTGTCCCGGCCTGTAG85240.12558666164752197No Hit
CTCGACACAAGGGTTTGT83940.12367133245768411No Hit
TAGCAGCACATAATGGTTTGT83510.12303780049489159No Hit
ACGCCTGTCTGAGCGTCGCT82910.12215380240727412No Hit
GGAATACCGGGTGCTGTAGGCTTT80770.11900087589477182No Hit
CCCATAAAGTAGAAAGCACTA80680.11886827618162918No Hit
TATTGCACTTGTCCCGGCCTGTAC79980.11783694507940881No Hit
CAGTGCAATAGTATTGTCAAAGC78000.11491975139027116No Hit
TTCACAGTGGCTAAGTTCTG77440.11409468650849484No Hit
CCCCCCACTGCTAAATTTGACTGG77090.11357902095738466No Hit
TGTAAACATCCTTGACTGGAAGC76540.11276868937706865No Hit
TTCACAGTGGCTAAGTTCCGC76300.11241509014202167No Hit
TGAGGTAGTAGGTTGTATAGT76240.11232669033325993No Hit
GGAATACCGGGTGCTGTAGGCTT73470.10824556582875926No Hit
GTCAGGATGGCCGAGCGGTCTAAGG72570.10691956869733305No Hit
TATTGCACTTGTCCCGGCCTGA72520.1068459021900316No Hit
ATCCCACCGCTGCCACCA72210.10638916984476257No Hit
ACCGGGTGCTGTAGGCTTT72080.10619763692577878No Hit
TGATGTAGTAGATTGTATAGTT71530.10538730534546277No Hit
AGAATTACTCTGAGACCT70290.10356037596438666No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT70130.10332464314102201No Hit
CAACGGAATCCCAAAAGCAGCTGA68680.10118831442927978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)