FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7390170
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG79336510.735409334291363No Hit
CAACGGAATCCCAAAAGCAGCTG6436708.709813170738968No Hit
GCCTGTCTGAGCGTCGCT5603667.582586056883671No Hit
CCCCCACTGCTAAATTTGACTG3629404.911118418114874No Hit
TTCAAGTAATCCAGGATAGGCT2350103.1800351006810397No Hit
TAGCAGCACGTAAATATTGGCG2323733.1443525656378672No Hit
AACATTCAACGCTGTCGGTGAGT1796532.4309724945434272No Hit
AAGCTGCCAGTTGAAGAACTGT1706022.308498992580685No Hit
AACATTCAACGCTGTCGGTGAG1516692.0523073217530854No Hit
TGAGGTAGTAGGTTGTATAGTT1026441.388926100482127No Hit
TAGCTTATCAGACTGATGTTGAC971631.3147600122865917No Hit
TAGCTTATCAGACTGATGTTGACT896721.213395632306158No Hit
CATAAAGTAGAAAGCACTACT781961.0581082708516856No Hit
TAGCTTATCAGACTGATGTTGA751021.0162418455867728No Hit
TATTGCACTTGTCCCGGCCTGTAA669210.9055407385757027No Hit
TGTAAACATCCTTGACTGGAAGCT636530.8613198343204554No Hit
TATTGCACTTGTCCCGGCCTGTAT628990.8511170920290061No Hit
TCCTGTCTGAGCGTCGCT604670.8182085121181244No Hit
TGAGGTAGTAGATTGTATAGTT536900.7265056148911324No Hit
TATTGCACTTGTCCCGGCCTGT388770.526063676478349No Hit
CAACGGAATCCCAAAAGCAGCTGT383130.5184319169924373No Hit
TTCAAGTAATTCAGGATAGGTT379400.5133846718005134No Hit
AGCAGCATTGTACAGGGCTATGA346900.46940733433736975No Hit
CCCATAAAGTAGAAAGCACTAC344690.46641687538987603No Hit
AACCGGGCGGAAACACCA340650.460950154055996No Hit
TCGTACGACTCTTAGCGG336080.4547662638342555No Hit
TGTAAACATCCCCGACTGGAAGCT328030.44387341563184607No Hit
TATTGCACTTGTCCCGGCCTGTA303960.41130312293221943No Hit
ACTGGACTTGGAGTCAGAAGGC291260.3941181326004679No Hit
TTCTCACTACTGCACTTGACTAG243650.3296947160890751No Hit
CCCATAAAGTAGAAAGCACT228940.30978989657883377No Hit
CATTGCACTTGTCTCGGTCTGA222170.30062907889804974No Hit
TGAGGTAGTAGTTTGTACAGTT220500.29836932032686664No Hit
TGTAAACATCCTACACTCTCAGCT212080.2869758070517999No Hit
CAACGGAATCCCAAAAGCAGCT209780.28386356470825436No Hit
CCCCCACTGCTAAATTTGACTGGCTT208800.2825374788401349No Hit
TAGCAGCACGTAAATATTGGC208750.28246982139788396No Hit
CACCCGGCTGTGTGCACATGTGC185970.2516450907083328No Hit
GGAATACCGGGTGCTGTAGGCTTT175480.2374505593240751No Hit
CAAAGAATTCTCCTTTTGGGCT168870.22850624545849418No Hit
AACGGAATCCCAAAAGCAGCTG161080.21796521595578994No Hit
CAACACCAGTCGATGGGCTGT158070.21389223793228032No Hit
CTTCTCACTACTGCACTTGACTAG154690.2093185948361134No Hit
CCCCCCACTGCTAAATTTGACTGG151610.20515089639345238No Hit
ACCCCCCACTGCTAAATTTGACTG144330.19529997280170822No Hit
TTCACAGTGGCTAAGTTCCGC138410.18728933163919098No Hit
TAGCTTATCAGACTGATGTTGACA129250.1748944882188096No Hit
GGAATACCGGGTGCTGTAGGCTT125910.17037497107644345No Hit
TGTCAGTTTGTCAAATACCCCA125280.16952248730408095No Hit
AACATTCAACGCTGTCGGTGAGTT116010.1569787975107474No Hit
CGCCTGTCTGAGCGTCGCT115650.15649166392654026No Hit
TGTAAACATCCTACACTCAGCT115580.15639694350738886No Hit
TGAGGTAGTAGTTTGTGCTGTT112010.15156620213066818No Hit
ACCGGGTGCTGTAGGCTT111200.15047015156620214No Hit
TGTCAGTTTGTCAAATACCCCAA110960.15014539584339737No Hit
TTCAAGTAATCCAGGATAGGCTT107740.1457882565624336No Hit
TTCAAGTAATCCAGGATAGGC105510.14277073463803944No Hit
ATCACATTGCCAGGGATTTCC104140.14091692072036233No Hit
CAAAGAATTCTCCTTTTGGGCTT103980.14070041690515914No Hit
TTCACAGTGGCTAAGTTCTGC98870.13378582630710795No Hit
CCCCCCACTGCTAAATTTGACTGGCT98590.13340694463050243No Hit
TGTAAACATCCCCGACTGGAAGC91930.12439497332267051No Hit
GACCCGCGGGCGCTCTCCAGTCCT91820.12424612694971834No Hit
ACTGGACTTGGAGTCAGAAGGCT88990.12041671571831229No Hit
CTCCCACTGCTTCACTTGACTAG86300.11677674532520903No Hit
TGAGGTAGTAGGTTGTGTGGTT84870.1148417424768307No Hit
CCCATAAAGTAGAAAGCACTA84020.11369156595856388No Hit
CACTAGATTGTGAGCTCCTGGA83730.11329915279350813No Hit
TTCACAGTGGCTAAGTTCCG83540.11304205451295438No Hit
TGAGGTAGTAGGTTGTATAGT81240.10992981216940882No Hit
ACCGGGTGCTGTAGGCTTT80310.1086713837435404No Hit
TAGCAGCACGTAAATATTGGCGT80180.10849547439368784No Hit
CACTAGATTGTGAGCTCCTGGAA80070.10834662802073566No Hit
TTATAAAGCAATGAGACTGATT79130.10707466810641704No Hit
TGATGTAGTAGGTTGTATAGTT79030.10693935322191506No Hit
TATTGCACTTGTCCCGGCCTGTT77420.10476078358143318No Hit
AGAGGTAGTAGGTTGCATAGTT75520.10218980077589554No Hit
CTACGCCTGTCTGAGCGTCGCT74940.10140497444578406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)