FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9075250
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG116527812.840175201785076No Hit
CAACGGAATCCCAAAAGCAGCTG8076008.898928404176193No Hit
CCCCCACTGCTAAATTTGACTG4441174.8937164265447235No Hit
TTCAAGTAATCCAGGATAGGCT3258563.590600809895044No Hit
TAGCAGCACGTAAATATTGGCG2864283.1561444588303353No Hit
GCCTGTCTGAGCGTCGCT2462252.7131483981157545No Hit
AAGCTGCCAGTTGAAGAACTGT2407042.6523126084680864No Hit
AACATTCAACGCTGTCGGTGAGT1875852.0669953995757693No Hit
AACATTCAACGCTGTCGGTGAG1670301.8405002617007795No Hit
TATTGCACTTGTCCCGGCCTGTAT1207441.3304757444698494No Hit
TATTGCACTTGTCCCGGCCTGTAA1162111.2805267072532438No Hit
TAGCTTATCAGACTGATGTTGAC1093071.2044516679981268No Hit
TGAGGTAGTAGGTTGTATAGTT1062441.1707005316657944No Hit
TAGCTTATCAGACTGATGTTGACT960481.0583510096140603No Hit
TGTAAACATCCTTGACTGGAAGCT844770.9308503897964244No Hit
CATAAAGTAGAAAGCACTACT815170.8982342084240104No Hit
TAGCTTATCAGACTGATGTTGA814360.8973416710283464No Hit
TATTGCACTTGTCCCGGCCTGT668210.7362992755020523No Hit
TGAGGTAGTAGATTGTATAGTT615170.6778546045563484No Hit
TATTGCACTTGTCCCGGCCTGTA555730.6123577862868792No Hit
TTCAAGTAATTCAGGATAGGTT547170.6029255392413433No Hit
CAACGGAATCCCAAAAGCAGCTGT524070.5774716949946282No Hit
GGAATACCGGGTGCTGTAGGCTTT494040.5443816974738989No Hit
AGCAGCATTGTACAGGGCTATGA467460.5150932481198865No Hit
CCCATAAAGTAGAAAGCACTAC467010.5145973940111842No Hit
AACCGGGCGGAAACACCA461770.5088234483898515No Hit
TGTAAACATCCCCGACTGGAAGCT398510.43911737968651No Hit
TTCTCACTACTGCACTTGACTAG361960.3988430070796948No Hit
GGAATACCGGGTGCTGTAGGCTT351290.387085755213355No Hit
TCGTACGACTCTTAGCGG333510.36749400842951985No Hit
ACTGGACTTGGAGTCAGAAGGC314450.34649183218093166No Hit
CACCCGGCTGTGTGCACATGTGC272710.30049860885375057No Hit
CATTGCACTTGTCTCGGTCTGA271980.2996942232996336No Hit
CCCCCACTGCTAAATTTGACTGGCTT248800.27415222721137156No Hit
TGTAAACATCCTACACTCTCAGCT243040.2678052946199829No Hit
TGATGTAGTAGGTTGTATAGTT241710.266339770254263No Hit
CCCATAAAGTAGAAAGCACT234590.2584942563565742No Hit
TAGCAGCACGTAAATATTGGC232550.25624638439712405No Hit
ACCCCCCACTGCTAAATTTGACTG232390.2560700807140299No Hit
TGAGGTAGTAGTTTGTACAGTT228950.25227955152750614No Hit
CTTCTCACTACTGCACTTGACTAG219930.24234043139307457No Hit
TGAGAACTGAATTCCATGGGTT216930.23903473733505962No Hit
TTAATGCTAATCGTGATAGGGGTT215570.23753615602875952No Hit
CAACGGAATCCCAAAAGCAGCT208680.2299440786755186No Hit
CAAAGAATTCTCCTTTTGGGCT205730.22669347951847058No Hit
CCCCCCACTGCTAAATTTGACTGG190640.21006583840665546No Hit
CTCCCACTGCTTCACTTGACTAG180460.19884851656979144No Hit
AACGGAATCCCAAAAGCAGCTG167050.18407206413046473No Hit
TAGCTTATCAGACTGATGTTGACA153500.1691413459684306No Hit
CTACGCCTGTCTGAGCGTCGCT152520.16806148590947909No Hit
TTCAAGTAATCCAGGATAGGCTT150650.16600093661331644No Hit
CAACACCAGTCGATGGGCTGT149850.16511941819784579No Hit
TTCAAGTAATCCAGGATAGGC148480.1636098179113523No Hit
TGTCAGTTTGTCAAATACCCCA146700.1616484394369301No Hit
GACCCGCGGGCGCTCTCCAGTCCT143510.15813338475524089No Hit
ACCGGGTGCTGTAGGCTT143050.15762651166634528No Hit
CACTAGATTGTGAGCTCCTGGA140350.15465138701413184No Hit
TGATGTAGTAGATTGTATAGTT137350.1513456929561169No Hit
TATTGCACTTGTCCCGGCCTGTT136140.15001239635271754No Hit
TGAGGTAGTAGTTTGTGCTGTT134970.14872317567009172No Hit
TGTCAGTTTGTCAAATACCCCAA134640.1483595493237101No Hit
GGGAATACCGGGTGCTGTAGGCTT132600.14611167736425992No Hit
CACTAGATTGTGAGCTCCTGGAA131280.14465717197873337No Hit
GGGAATACCGGGTGCTGTAGGCTTT130630.14394093826616347No Hit
CGCCTGTCTGAGCGTCGCT130450.14374259662268257No Hit
TATTGCACTTGTCCCGGCCTGTAG129690.14290515412798543No Hit
AACATTCAACGCTGTCGGTGAGTT127660.14066830114872866No Hit
CCCCCCACTGCTAAATTTGACTGGCT126000.1388391504366271No Hit
AGCTCGGTCTGAGGCCCCTCAGT123250.13580893088344673No Hit
CAAAGAATTCTCCTTTTGGGCTT122530.13501556430952316No Hit
ATCACATTGCCAGGGATTTCC121750.13415608385443928No Hit
TGTAAACATCCCCGACTGGAAGC120250.1325032368254318No Hit
ACCGGGTGCTGTAGGCTTA119080.13121401614280598No Hit
TTCACAGTGGCTAAGTTCTGC118580.13066306713313683No Hit
TTCACAGTGGCTAAGTTCCGC118130.1301672130244346No Hit
ACCGGGTGCTGTAGGCTTT114300.12594694361036884No Hit
CCCATAAAGTAGAAAGCACTA112070.12348971102724443No Hit
TTCACAGTGGCTAAGTTCCG110710.12199112972094432No Hit
TGAGAACTGAATTCCATAGGCTGT110060.12127489600837443No Hit
GAATACCGGGTGCTGTAGGCTTT108980.12008484614748904No Hit
AGCAGCATTGTACAGGGCTATGT107950.1189498911875706No Hit
TAGCAGCACGTAAATATTGGCGT106480.11733010109914327No Hit
TGTAAACATCCTACACTCAGCT105110.1158205008126498No Hit
TTATAAAGCAATGAGACTGATT101420.11175449712129142No Hit
TCGTTTCCCGGCCAATGCACCA99740.10990330844880307No Hit
TATTGCACTTGTCCCGGCCTGTAAT98350.10837167020192282No Hit
TGAGGTAGTAGGTTGTATAGT91560.10088978265061568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)