FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6733436
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG82058812.18676467705344No Hit
CAACGGAATCCCAAAAGCAGCTG5083087.5490136090994255No Hit
CCCCCACTGCTAAATTTGACTG3369205.003686082410229No Hit
TTCAAGTAATCCAGGATAGGCT2313663.4360763212125276No Hit
GCCTGTCTGAGCGTCGCT1841202.7344137525031798No Hit
AAGCTGCCAGTTGAAGAACTGT1822012.7059141870510093No Hit
TAGCAGCACGTAAATATTGGCG1659642.4647742994809785No Hit
AACATTCAACGCTGTCGGTGAGT1632172.4239778918222434No Hit
AACATTCAACGCTGTCGGTGAG1352042.007949581758852No Hit
TAGCTTATCAGACTGATGTTGAC990421.4708983645199867No Hit
TATTGCACTTGTCCCGGCCTGTAT807631.1994322066772447No Hit
TAGCTTATCAGACTGATGTTGACT806591.1978876757720724No Hit
TATTGCACTTGTCCCGGCCTGTAA802271.1914719320121259No Hit
TGAGGTAGTAGGTTGTATAGTT696151.033870374649733No Hit
TAGCTTATCAGACTGATGTTGA676901.0052817016453413No Hit
TGTAAACATCCTTGACTGGAAGCT635470.9437529368364086No Hit
CATAAAGTAGAAAGCACTACT594090.8822984283209939No Hit
TATTGCACTTGTCCCGGCCTGT505170.7502410359287591No Hit
TTCAAGTAATTCAGGATAGGTT443880.6592176713345164No Hit
TGAGGTAGTAGATTGTATAGTT406540.6037630713353479No Hit
TATTGCACTTGTCCCGGCCTGTA372570.5533133455192861No Hit
CAACGGAATCCCAAAAGCAGCT372400.5530608741213252No Hit
AGCAGCATTGTACAGGGCTATGA362730.5386997069549633No Hit
CAACGGAATCCCAAAAGCAGCTGT327600.48652723512928614No Hit
TGTAAACATCCCCGACTGGAAGCT303180.45026046137514336No Hit
CCCATAAAGTAGAAAGCACTAC294940.43802302420339334No Hit
ACTGGACTTGGAGTCAGAAGGC268700.39905332136519894No Hit
TTCTCACTACTGCACTTGACTAG234940.34891547198191236No Hit
CACCCGGCTGTGTGCACATGTGC221390.32879201643856126No Hit
AACCGGGCGGAAACACCA215550.3201188813556704No Hit
CATTGCACTTGTCTCGGTCTGA210260.3122625655014765No Hit
CCCATAAAGTAGAAAGCACT205410.30505970503024016No Hit
TGAGAACTGAATTCCATGGGTT196680.2920945561820147No Hit
TGTAAACATCCTACACTCTCAGCT182010.27030775966386256No Hit
TCGTACGACTCTTAGCGG177890.2641890410779875No Hit
ACCGGGTGCTGTAGGCTT177860.2641444873018768No Hit
TTAATGCTAATCGTGATAGGGGTT171940.2553525421493573No Hit
TCCTGTCTGAGCGTCGCT171140.25416444145307093No Hit
TGAGGTAGTAGTTTGTACAGTT170190.2527535718762308No Hit
ACCCCCCACTGCTAAATTTGACTG154300.22915492179624192No Hit
CCCCCACTGCTAAATTTGACTGGCTT151220.22458073411553925No Hit
TCGTTTCCCGGCCAATGCACCA151210.22456588285683565No Hit
CAAAGAATTCTCCTTTTGGGCT137610.20436817101996663No Hit
TAGCTTATCAGACTGATGTTGACA133330.19801183229483432No Hit
AACGGAATCCCAAAAGCAGCTG132940.1974326332053947No Hit
CTTCTCACTACTGCACTTGACTAG132520.1968088803398443No Hit
CCCCCCACTGCTAAATTTGACTGG130300.19351190090764953No Hit
TAGCAGCACGTAAATATTGGC125680.1866506193865955No Hit
CAACACCAGTCGATGGGCTGT124660.18513579099883032No Hit
ACCGGGTGCTGTAGGCTTT124370.1847051044964265No Hit
TTCAAGTAATCCAGGATAGGCTT121120.17987844541776293No Hit
TGAGAACTGAATTCCATAGGCTGT119580.1775913515774116No Hit
CTCCCACTGCTTCACTTGACTAG116150.1724973698420836No Hit
ACCGGGTGCTGTAGGCTTA115380.17135382292190793No Hit
CGCCTGTCTGAGCGTCGCT110400.163957896087525No Hit
CACTAGATTGTGAGCTCCTGGA107260.15929460085460084No Hit
TGAGGTAGTAGTTTGTGCTGTT106870.15871540176516122No Hit
TTCAAGTAATCCAGGATAGGC104340.15495803331315544No Hit
TGTCAGTTTGTCAAATACCCCA101590.15087393716967087No Hit
GGAATACCGGGTGCTGTAGGCTTT101570.1508442346522637No Hit
AACATTCAACGCTGTCGGTGAGTT97780.14521560760360683No Hit
TATTGCACTTGTCCCGGCCTGTT97380.14462155725546363No Hit
AGCTCGGTCTGAGGCCCCTCAGT96900.14390869683769178No Hit
TTCACAGTGGCTAAGTTCTGC96790.1437453329919524No Hit
CACTAGATTGTGAGCTCCTGGAA96610.14347801033528795No Hit
GGAATACCGGGTGCTGTAGGCTT93060.138205813495517No Hit
CAAAGAATTCTCCTTTTGGGCTT91760.13627514986405156No Hit
TATTGCACTTGTCCCGGCCTGTAG90600.1345524038544363No Hit
TGTAAACATCCCCGACTGGAAGC89790.13334945189944628No Hit
TGTCAGTTTGTCAAATACCCCAA88800.13187917728779186No Hit
CAACGGAATCCCAAAAGCAGCTGA87310.1296663397409584No Hit
CCCCCCACTGCTAAATTTGACTGGCT86770.12886437177096507No Hit
AATGCACCCGGGCAAGGATTCT86070.12782478366171446No Hit
TGATGTAGTAGGTTGTATAGTT83620.12418622527933733No Hit
AGCAGCATTGTACAGGGCTATGT81950.12170606507583943No Hit
GACCCGCGGGCGCTCTCCAGTCCT81450.12096350214066043No Hit
TTATAAAGCAATGAGACTGATT81120.12047341060344229No Hit
TGTAAACATCCTACACTCAGCT78200.11613684306199688No Hit
CCCATAAAGTAGAAAGCACTA76320.1133448064257238No Hit
ATCACATTGCCAGGGATTTCC73210.10872606496891038No Hit
TTCACAGTGGCTAAGTTCCG72790.10810231210336No Hit
TTCACAGTGGCTAAGTTCCGC72010.10694391392448074No Hit
GGGAATACCGGGTGCTGTAGGCTT71200.10574096196949076No Hit
TATTGCACTTGTCCCGGCCTGTAAT69830.10370633952710027No Hit
TGAGGTAGTAGGTTGTGTGGTT69520.1032459505072893No Hit
CAAAGTGCTGTTCGTGCAGGTAG69210.1027855614874783No Hit
CTACGCCTGTCTGAGCGTCGCT67890.10082519533860573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)