FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6965994
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG120242017.26128388855919No Hit
CAACGGAATCCCAAAAGCAGCTG5255577.544608852663382No Hit
CCCCCACTGCTAAATTTGACTG4066695.837917747273397No Hit
TTCAAGTAATCCAGGATAGGCT2159203.0996294283342767No Hit
TAGCAGCACGTAAATATTGGCG2031012.9156068753432747No Hit
AAGCTGCCAGTTGAAGAACTGT1618622.32360234591072No Hit
AACATTCAACGCTGTCGGTGAGT1540182.2109981719766054No Hit
AACATTCAACGCTGTCGGTGAG1132271.6254248855224394No Hit
TAGCTTATCAGACTGATGTTGAC1113661.598709387346587No Hit
TAGCTTATCAGACTGATGTTGACT859671.2340952346499294No Hit
TATTGCACTTGTCCCGGCCTGTAA758891.08942097854233No Hit
TATTGCACTTGTCCCGGCCTGTAT717861.0305205545683789No Hit
TGTAAACATCCTTGACTGGAAGCT705581.0128920581901162No Hit
TAGCTTATCAGACTGATGTTGA601240.8631072607871899No Hit
TGAGGTAGTAGGTTGTATAGTT544230.7812668227965743No Hit
CATAAAGTAGAAAGCACTACT464230.6664231981824847No Hit
TATTGCACTTGTCCCGGCCTGT353270.5071350908427426No Hit
TATTGCACTTGTCCCGGCCTGTA350520.5031873412466333No Hit
CAACGGAATCCCAAAAGCAGCTGT349990.502426502233565No Hit
AGCAGCATTGTACAGGGCTATGA332740.4776633456761519No Hit
GGAATACCGGGTGCTGTAGGCTTT322630.46314998261554635No Hit
TGAGGTAGTAGATTGTATAGTT319350.45844139400636863No Hit
TGTAAACATCCCCGACTGGAAGCT317270.4554554597664023No Hit
TTCAAGTAATTCAGGATAGGTT313950.45068944934491767No Hit
TTCTCACTACTGCACTTGACTAG293470.4212894814437107No Hit
CCCATAAAGTAGAAAGCACTAC276110.3963684149024533No Hit
ACTGGACTTGGAGTCAGAAGGC272570.39128658451327974No Hit
CAACGGAATCCCAAAAGCAGCT249030.3574938479705839No Hit
TTAATGCTAATCGTGATAGGGGTT239870.3443442529522707No Hit
CCCCCACTGCTAAATTTGACTGGCTT237480.34091329966692474No Hit
GGAATACCGGGTGCTGTAGGCTT228670.3282661455062982No Hit
TGTAAACATCCTACACTCTCAGCT222990.3201122481586978No Hit
ACCCCCCACTGCTAAATTTGACTG220460.3164803185302772No Hit
CTTCTCACTACTGCACTTGACTAG213680.30674732134423316No Hit
CAAAGAATTCTCCTTTTGGGCT204330.2933249727174614No Hit
CCCCCCACTGCTAAATTTGACTGG192530.2763855380868832No Hit
TAGCTTATCAGACTGATGTTGACA168070.24127209986112533No Hit
CCCATAAAGTAGAAAGCACT166770.2394058909611464No Hit
TAGCAGCACGTAAATATTGGC155120.22268178812671963No Hit
CATTGCACTTGTCTCGGTCTGA145520.20890055317302883No Hit
CACCCGGCTGTGTGCACATGTGC137280.19707165983777766No Hit
CCCCCCACTGCTAAATTTGACTGGCT132170.18973602331555267No Hit
AACATTCAACGCTGTCGGTGAGTT128540.18452499384868837No Hit
TGAGGTAGTAGTTTGTACAGTT123090.1767012719218535No Hit
TGTCAGTTTGTCAAATACCCCA118450.17004034169423632No Hit
AACGGAATCCCAAAAGCAGCTG116940.16787266827964537No Hit
CACTAGATTGTGAGCTCCTGGAA113200.16250372882893668No Hit
CAAAGAATTCTCCTTTTGGGCTT112250.16113996078664436No Hit
TCGTACGACTCTTAGCGG109830.15766594114206817No Hit
TTCAAGTAATCCAGGATAGGCTT108660.1559863531320871No Hit
GACCCGCGGGCGCTCTCCAGTCCT104310.14974173104369598No Hit
CACTAGATTGTGAGCTCCTGGA102730.1474735694575677No Hit
TGTCAGTTTGTCAAATACCCCAA98200.1409705492137949No Hit
CTCCCACTGCTTCACTTGACTAG93510.1342378417207939No Hit
ACCGGGTGCTGTAGGCTT92390.13263003097619663No Hit
TGAATACCGGGTGCTGTAGGCTTT90980.13060591209237332No Hit
ATCACATTGCCAGGGATTTCC87800.12604087801396324No Hit
TATTGCACTTGTCCCGGCCTGTAG86150.12367222825629767No Hit
TTCAAGTAATCCAGGATAGGC86100.12360045099091385No Hit
TTCACAGTGGCTAAGTTCCG86070.12355738463168359No Hit
TGTAAACATCCCCGACTGGAAGC85470.12269605744707791No Hit
AGCTCGGTCTGAGGCCCCTCAGT85310.12246637019784973No Hit
TATTGCACTTGTCCCGGCCTGTT83750.12022691951787498No Hit
CCCCCCACTGCTAAATTTGACTGTA82420.11831764425866574No Hit
CGCCCCACTGCTAAATTTGACTG82360.11823151154020518No Hit
TGTAAACATCCTACACTCAGCT80280.11524557730023884No Hit
ACTGGACTTGGAGTCAGAAGGCA77300.11096765228336401No Hit
TGAGGTAGTAGTTTGTGCTGTT77240.11088151956490346No Hit
CCCCCCACTGCTAAATTTGACTGT76940.11045085597260061No Hit
CAACACCAGTCGATGGGCTGT76360.10961823969414847No Hit
CAACGGAATCCCAAAAGCAGCTGA76300.10953210697568788No Hit
CTACGCCTGTCTGAGCGTCGCT75840.10887175613415688No Hit
CCCATAAAGTAGAAAGCACTA75630.1085702916195449No Hit
TAGCAGCACGTAAATATTGGCGT74490.10693376996879411No Hit
AATGCACCCGGGCAAGGATTCT72300.10378992574498341No Hit
TATTGCACTTGTCCCGGCCTGTAAT72220.10367508212036933No Hit
TTCACAGTGGCTAAGTTCTGC71150.10213904864115589No Hit
ACCGGGTGCTGTAGGCTTT70910.10179451776731362No Hit
ATCACATTGCCAGGGATTTCCA70120.10066043697424948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)