FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11232932
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG138993112.373715072787764No Hit
CAACGGAATCCCAAAAGCAGCTG6539875.82205073439419No Hit
CCCCCACTGCTAAATTTGACTG4900814.362894745557082No Hit
TTCAAGTAATCCAGGATAGGCT3524253.1374266309098995No Hit
AACATTCAACGCTGTCGGTGAGT3205032.8532443711045343No Hit
TAGCAGCACGTAAATATTGGCG3119552.7771466968730874No Hit
AAGCTGCCAGTTGAAGAACTGT2856502.5429691909467627No Hit
TAGCTTATCAGACTGATGTTGAC1998451.7790991701899377No Hit
AACATTCAACGCTGTCGGTGAG1900731.6921049642248345No Hit
TATTGCACTTGTCCCGGCCTGTAA1566861.3948806954408697No Hit
TAGCTTATCAGACTGATGTTGACT1548631.37865162897808No Hit
TATTGCACTTGTCCCGGCCTGTAT1522231.3551493056309787No Hit
GCCTGTCTGAGCGTCGCT1395891.2426764445827678No Hit
TGTAAACATCCTTGACTGGAAGCT1332241.1860126990887152No Hit
TGAGGTAGTAGGTTGTATAGTT1193221.06225160091773No Hit
TAGCTTATCAGACTGATGTTGA1067380.9502238596298812No Hit
AGCAGCATTGTACAGGGCTATGA860660.766193545905913No Hit
TATTGCACTTGTCCCGGCCTGT768670.6843004123945556No Hit
TGAGGTAGTAGATTGTATAGTT714800.636343209413179No Hit
TATTGCACTTGTCCCGGCCTGTA701450.6244585118115199No Hit
TGTAAACATCCCCGACTGGAAGCT669860.5963358453518636No Hit
CATAAAGTAGAAAGCACTACT667920.59460878068166No Hit
ACTGGACTTGGAGTCAGAAGGC647870.5767594782911531No Hit
TTCAAGTAATTCAGGATAGGTT545640.48575029208758674No Hit
AACCGGGCGGAAACACCA527540.46963695676249084No Hit
TTCTCACTACTGCACTTGACTAG437680.3896400334302745No Hit
TGTAAACATCCTACACTCTCAGCT437160.38917710887949825No Hit
CAACGGAATCCCAAAAGCAGCTGT416430.3707224436148995No Hit
CCCATAAAGTAGAAAGCACTAC410220.3651940561912064No Hit
CTTCTCACTACTGCACTTGACTAG341660.3041592346504012No Hit
CCCCCACTGCTAAATTTGACTGGCTT307210.27349048316147556No Hit
CGCCTGTCTGAGCGTCGCT305500.27196817358103836No Hit
TGAGGTAGTAGTTTGTACAGTT283600.25247192807719304No Hit
AACATTCAACGCTGTCGGTGAGTT280460.24967657598212115No Hit
CATTGCACTTGTCTCGGTCTGA274400.24428172448653654No Hit
TAGCTTATCAGACTGATGTTGACA270180.24052491370908324No Hit
CCCATAAAGTAGAAAGCACT248560.22127793527104053No Hit
CAAAGAATTCTCCTTTTGGGCT247720.22053013407363278No Hit
CACCCGGCTGTGTGCACATGTGC246910.2198090400618467No Hit
ACCCCCCACTGCTAAATTTGACTG243500.21677332329617946No Hit
TTCAAGTAATCCAGGATAGGCTT243240.21654186102079137No Hit
CCCCCCACTGCTAAATTTGACTGG242190.21560710952403167No Hit
GACCCGCGGGCGCTCTCCAGTCCT226080.2012653508451756No Hit
TCGTACGACTCTTAGCGG220100.1959417185112489No Hit
CAACGGAATCCCAAAAGCAGCT209410.18642505803471437No Hit
TGTAAACATCCTACACTCAGCT208570.1856772568373066No Hit
GGAATACCGGGTGCTGTAGGCTTT208210.18535677060984612No Hit
TGTAAACATCCCCGACTGGAAGC198910.17707754306711732No Hit
TAGCAGCACGTAAATATTGGC197420.17575108618123925No Hit
TGTCAGTTTGTCAAATACCCCA192280.1711752550447203No Hit
CTCCCACTGCTTCACTTGACTAG192220.17112184067347688No Hit
CCCCCCACTGCTAAATTTGACTGGCT189470.1686736819914872No Hit
CAAAGAATTCTCCTTTTGGGCTT188820.1680950263030169No Hit
AGCAGCATTGTACAGGGCTATGT187710.16710686043501377No Hit
CTACGCCTGTCTGAGCGTCGCT187590.16700003169252692No Hit
AGCTCGGTCTGAGGCCCCTCAGT186340.16588723229162253No Hit
ACTGGACTTGGAGTCAGAAGGCA182310.1622995670231067No Hit
TGTCAGTTTGTCAAATACCCCAA180550.1607327454666333No Hit
TATTGCACTTGTCCCGGCCTGTAG176320.15696703229397274No Hit
TGAGGTAGTAGTTTGTGCTGTT175570.15629935265343012No Hit
GGAATACCGGGTGCTGTAGGCTT172280.1533704646302497No Hit
TTCACAGTGGCTAAGTTCCG170550.15183035025939798No Hit
ACCGGGTGCTGTAGGCTT170080.15141193768465794No Hit
TATTGCACTTGTCCCGGCCTGTT170070.1514030352894507No Hit
CGCGACCTCAGATCAGACGTGGCG169140.1505751125351778No Hit
TACGCCTGTCTGAGCGTCGCT168230.1497649945713194No Hit
GTCAGGATGGCCGAGCGGTCTAAGG159930.14237600654931412No Hit
AACGGAATCCCAAAAGCAGCTG157560.14026613888519934No Hit
CACTAGATTGTGAGCTCCTGGAA153190.1363757921796375No Hit
ACTGGACTTGGAGTCAGAAGGCT153190.1363757921796375No Hit
TTCAAGTAATCCAGGATAGGC152620.1358683556528251No Hit
ACTGGACTTGGAGTCAGAAGG151090.1345062891861181No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC143410.1276692496669614No Hit
TAGCAGCACGTAAATATTGGCGT142330.12670779098458No Hit
TTATAAAGCAATGAGACTGATT140020.12465133769170864No Hit
TCCTGTCTGAGCGTCGCT139180.12390353649430086No Hit
CACTAGATTGTGAGCTCCTGGA138780.12354744068601145No Hit
ACCGGGTGCTGTAGGCTTA134190.11946124128589045No Hit
GGGCTACGCCTGTCTGAGCGTCGCT133360.11872234248368992No Hit
ATCACATTGCCAGGGATTTCCA130490.1161673550592134No Hit
CAACACCAGTCGATGGGCTGT129110.11493882452061491No Hit
AGCAGCATTGTACAGGGCTATG128060.1140040730238552No Hit
CCCCCCACTGCTAAATTTGACTGTA123210.1096864113483461No Hit
GTGCGGTAACGCGACCGATCCCGGA121800.10843117362412592No Hit
CAAAGTGCTGTTCGTGCAGGTAG121790.10842227122891868No Hit
TATTGCACTTGTCCCGGCCTGTAAT120500.10727386224718533No Hit
TATTGCACTTGTCCCGGCCTGTTT117930.10498594667892586No Hit
GCGTTCGCGCTTTCCCCTG116700.1038909520684359No Hit
TTCACAGTGGCTAAGTTCTGC116530.10373961134991293No Hit
ATCACATTGCCAGGGATTTCC114890.10227961853592632No Hit
ACCGGGTGCTGTAGGCTTT114470.10190571793722245No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT113840.10134486703916662No Hit
GGGAATACCGGGTGCTGTAGGCTT113700.10122023350626533No Hit
GGGAATACCGGGTGCTGTAGGCTTT113340.10089974727880485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)