FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9141966
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7992538.742681825769205No Hit
CAACGGAATCCCAAAAGCAGCTG6460567.066926304473239No Hit
GCCTGTCTGAGCGTCGCT3781784.136725076422292No Hit
CCCCCACTGCTAAATTTGACTG2946273.2227969344887084No Hit
TTCAAGTAATCCAGGATAGGCT2169072.372651571882897No Hit
TAGCAGCACGTAAATATTGGCG2165662.368921520819482No Hit
AACATTCAACGCTGTCGGTGAGT2128562.328339440334825No Hit
AAGCTGCCAGTTGAAGAACTGT1792841.9611098969302663No Hit
AACATTCAACGCTGTCGGTGAG1740961.9043606156487565No Hit
TATTGCACTTGTCCCGGCCTGTAT1612041.7633406206061149No Hit
TATTGCACTTGTCCCGGCCTGTAA1509801.6515047201006872No Hit
TCCTGTCTGAGCGTCGCT1343821.4699463988380619No Hit
TATTGCACTTGTCCCGGCCTGT1222051.3367474786058053No Hit
TAGCTTATCAGACTGATGTTGAC1028951.125523765894557No Hit
TGAGGTAGTAGGTTGTATAGTT1024011.1201201142073818No Hit
TAGCTTATCAGACTGATGTTGACT1011961.1069391419744943No Hit
TATTGCACTTGTCCCGGCCTGTA792780.8671876486961337No Hit
TAGCTTATCAGACTGATGTTGA725460.7935492212506587No Hit
TGAGGTAGTAGATTGTATAGTT604630.6613785262382292No Hit
TTCAAGTAATTCAGGATAGGTT553710.605679347308883No Hit
TGTAAACATCCTTGACTGGAAGCT543280.5942704227952719No Hit
AGCAGCATTGTACAGGGCTATGA522800.5718682392824476No Hit
ACCGGGTGCTGTAGGCTTA512520.5606233932613619No Hit
TGTAAACATCCCCGACTGGAAGCT436510.4774793518155723No Hit
CATAAAGTAGAAAGCACTACT430280.470664625092677No Hit
CACCCGGCTGTGTGCACATGTGC418770.45807433543288173No Hit
ACCGGGTGCTGTAGGCTT389620.4261884150520796No Hit
CAACGGAATCCCAAAAGCAGCTGT349340.3821278705258803No Hit
ACCGGGTGCTGTAGGCTTT337310.36896877542532974No Hit
CATTGCACTTGTCTCGGTCTGA324410.35485802506813086No Hit
ACTGGACTTGGAGTCAGAAGGC315730.34536334963398463No Hit
GGAATACCGGGTGCTGTAGGCTTT301790.33011498839527514No Hit
TGATGTAGTAGGTTGTATAGTT290010.3172293574489339No Hit
CTCCCACTGCTTCACTTGACTAG284590.3113006545856767No Hit
TCGTACGACTCTTAGCGG279190.3053938288547562No Hit
CGCGACCTCAGATCAGACGTGG239340.26180364267379685No Hit
CCCATAAAGTAGAAAGCACTAC237270.25953935947694406No Hit
TTCTCACTACTGCACTTGACTAG233800.25574367701651923No Hit
ACCCCCCACTGCTAAATTTGACTG225290.2464349572072353No Hit
ATCCCGGACGAGCCCCCA224970.24608492308984742No Hit
CACTAGATTGTGAGCTCCTGGA222820.24373313136364763No Hit
CCCCCACTGCTAAATTTGACTGGCTT222770.24367843853280577No Hit
TATTGCACTTGTCCCGGCCTGTT221420.24220173210007562No Hit
TGAGGTAGTAGTTTGTACAGTT219770.24039686868229437No Hit
AGCTCGGTCTGAGGCCCCTCAGT215410.2356276538328845No Hit
CAACACCAGTCGATGGGCTGT212660.23261954813658245No Hit
TATTGCACTTGTCCCGGCCTGTAG208900.22850664725727485No Hit
GGAATACCGGGTGCTGTAGGCTT205800.22511569174507978No Hit
AACCGGGCGGAAACACCA205120.22437186924563055No Hit
CGCGACCTCAGATCAGACGTGGCG200340.21914323461714907No Hit
CACTAGATTGTGAGCTCCTGGAA195640.2140021085180146No Hit
CAACGGAATCCCAAAAGCAGCT194880.2131707774892184No Hit
CCCATAAAGTAGAAAGCACT194810.2130942075260398No Hit
TGATGTAGTAGATTGTATAGTT173370.1896419216610519No Hit
CTTCTCACTACTGCACTTGACTAG151150.16533642763493103No Hit
CCCCCCACTGCTAAATTTGACTGG150960.165128594877732No Hit
CGCCTGTCTGAGCGTCGCT149670.1637175198420121No Hit
TATTGCACTTGTCCCGGCCTGTTT149260.16326903862910888No Hit
CAAAGAATTCTCCTTTTGGGCT144580.15814978966231116No Hit
AACATTCAACGCTGTCGGTGAGTT138460.15145538716726797No Hit
TAGCAGCACGTAAATATTGGC137340.15023026775641038No Hit
CCCCCCACTGCTAAATTTGACTGT136170.14895045551471095No Hit
CGCGACCTCAGATCAGACGTGGC133580.1461173668771028No Hit
TGTAAACATCCTACACTCTCAGCT133060.1455485614363475No Hit
TAGCTTATCAGACTGATGTTGACA131840.14421405636380621No Hit
AACGGAATCCCAAAAGCAGCTG131660.14401716217277552No Hit
TGAGGTAGTAGTTTGTGCTGTT129040.14115125783666227No Hit
TGTAAACATCCCCGACTGGAAGC122450.13394274273170564No Hit
CCCCCCACTGCTAAATTTGACTGGCT120990.13234571207112342No Hit
TGAATACCGGGTGCTGTAGGCTTT120740.13207224791691416No Hit
TATTGCACTTGTCCCGGCCTGA119330.1305299100871738No Hit
TATTGCACTTGTCCCGGCCTGTAAT118720.12986265755090318No Hit
ACCTGGTGCTGTAGGCTTA116790.1277515142804075No Hit
CAAAGAATTCTCCTTTTGGGCTT113690.12436055876821243No Hit
TTCAAGTAATCCAGGATAGGC108750.11895690708103705No Hit
TGAGAACTGAATTCCATAGGCTGT108530.11871625862533289No Hit
TCGTTTCCCGGGCGGCGCACCA108400.11857405726514406No Hit
TTCAAGTAATCCAGGATAGGCTT104150.11392516664358629No Hit
CCCTGTCTGAGCGTCGCT102860.1125140916078664No Hit
TTATAAAGCAATGAGACTGATT98770.10804021804500256No Hit
AGCAGCATTGTACAGGGCTATGT96830.10591813620833856No Hit
TATTGCACTTGTCCCGGCCTGTAAA95010.103927317165695No Hit
TTCACAGTGGCTAAGTTCCGC93820.1026256277916588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)