FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5734930
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3992706.962072771594423No Hit
CCCCCCACTGCTAAATTTGACTG3879006.7638140308600105No Hit
AAGCTGCCAGTTGAAGAACTGT2010123.505047140941563No Hit
TTCAAGTAATCCAGGATAGGCT1734293.0240822468626467No Hit
TAGCAGCACGTAAATATTGGCG1502342.619630928363555No Hit
AACATTCAACGCTGTCGGTGAGT1493942.604983844615366No Hit
CCCCCACTGCTAAATTTGACTG1488122.5948355080184062No Hit
GCCTGTCTGAGCGTCGCT1229352.143618143551883No Hit
TAGCTTATCAGACTGATGTTGAC915481.596322884499026No Hit
AACATTCAACGCTGTCGGTGAG760901.3267816695234291No Hit
TGAGGTAGTAGGTTGTATAGTT755061.3165984589175457No Hit
TATTGCACTTGTCCCGGCCTGT637891.112289077634775No Hit
TATTGCACTTGTCCCGGCCTGTAA609891.0634654651408126No Hit
TAGCTTATCAGACTGATGTTGACT601851.0494461135532605No Hit
TATTGCACTTGTCCCGGCCTGTAT510720.8905426918898748No Hit
TAGCTTATCAGACTGATGTTGA507930.8856777676449407No Hit
TGTAAACATCCTTGACTGGAAGCT471660.8224337524607973No Hit
AGCAGCATTGTACAGGGCTATGA448050.7812649849257096No Hit
TGAGGTAGTAGATTGTATAGTT389990.680025737018586No Hit
CATAAAGTAGAAAGCACTACT367750.6412458390948103No Hit
ACCGGGTGCTGTAGGCTT366380.6388569694834985No Hit
CGCCTGTCTGAGCGTCGCT363540.6339048602162538No Hit
TATTGCACTTGTCCCGGCCTGTA302980.5283063611935979No Hit
ACTGGACTTGGAGTCAGAAGGC293550.5118632659858098No Hit
TGTAAACATCCCCGACTGGAAGCT276220.4816449372529394No Hit
ACCGGGTGCTGTAGGCTTT269110.4692472270803654No Hit
CAACGGAATCCCAAAAGCAGCTGT263680.45977893365742917No Hit
CCCATAAAGTAGAAAGCACTAC260000.45336211601536547No Hit
TTCAAGTAATTCAGGATAGGTT256740.44767765256071124No Hit
CGCGACCTCAGATCAGACGTGG228330.39813912288380154No Hit
ACCGGGTGCTGTAGGCTTA219070.38199245675186966No Hit
CATTGCACTTGTCTCGGTCTGA186020.3243631570045319No Hit
CGCGACCTCAGATCAGACGTGGCG185250.3230205076609479No Hit
TGAGGTAGTAGTTTGTACAGTT181800.3170047411215132No Hit
CCCCCACTGCTAAATTTGACTGGCTT180220.3142496944164968No Hit
TTCTCACTACTGCACTTGACTAG171060.2982773983291862No Hit
TTAATGCTAATCGTGATAGGGGTT168270.29341247408425214No Hit
TGTAAACATCCTACACTCTCAGCT153660.267937010565081No Hit
CTACGCCTGTCTGAGCGTCGCT152120.2652517118779131No Hit
GGAATACCGGGTGCTGTAGGCTTT152050.26512965284667817No Hit
TACGCCTGTCTGAGCGTCGCT151900.26486809777974624No Hit
CACCCGGCTGTGTGCACATGTGC140110.2443098695188956No Hit
AGCTCGGTCTGAGGCCCCTCAGT138030.24068297259077268No Hit
CTCCCACTGCTTCACTTGACTAG136970.23883465011778696No Hit
TGAGGTAGTAGTTTGTGCTGTT130380.22734366417724367No Hit
AACATTCAACGCTGTCGGTGAGTT126550.22066529146824806No Hit
GCGTTCGCGCTTTCCCCTG124030.21627116634379148No Hit
CCCATAAAGTAGAAAGCACT117850.2054950975861955No Hit
ACCCCCCACTGCTAAATTTGACTG116830.20371652313105829No Hit
TAGCTTATCAGACTGATGTTGACA112590.19632323323911538No Hit
GGGCTACGCCTGTCTGAGCGTCGCT112070.19541650900708465No Hit
CTTCTCACTACTGCACTTGACTAG112060.19539907200262252No Hit
GTCAGGATGGCCGAGCGGTCTAAGG109840.1915280570120298No Hit
GTGCGGTAACGCGACCGATCCCGGA109760.19138856097633275No Hit
GGAATACCGGGTGCTGTAGGCTT104030.18139715741953258No Hit
CCCCCCACTGCTAAATTTGACTGG103820.18103098032582787No Hit
CCCCCCACTGCTAAATTTGACTGGCT100440.17513727281762811No Hit
AACGGAATCCCAAAAGCAGCTG97900.17070827368424724No Hit
ACGCCTGTCTGAGCGTCGCT96240.16781373094353375No Hit
CAACACCAGTCGATGGGCTGT95800.16704650274720004No Hit
CAAAGAATTCTCCTTTTGGGCT93210.16253031859150852No Hit
TATTGCACTTGTCCCGGCCTGTT93040.16223388951565232No Hit
CGCGACCTCAGATCAGACGTGGC92520.1613271652836216No Hit
TGAGGTAGTAGGTTGTGTGGTT91600.15972296087310567No Hit
TTCAAGTAATCCAGGATAGGCTT88190.15377694235151954No Hit
CAACGGAATCCCAAAAGCAGCT81390.14191977931727154No Hit
CAAAGAATTCTCCTTTTGGGCTT80870.1410130550852408No Hit
TTCAAGTAATCCAGGATAGGC79330.13832775639807288No Hit
CGCGACCTCAGATCAGACGTG77460.13506703656365465No Hit
TGTAAACATCCCCGACTGGAAGC75770.1321201828095548No Hit
TAGCAGCACGTAAATATTGGC74760.1303590453588797No Hit
TGTAAACATCCTACACTCAGCT72930.12716807354231002No Hit
CAAAGTGCTGTTCGTGCAGGTAG72290.12605210525673374No Hit
TGAGGTAGTAGGTTGTATAGT72210.1259126092210367No Hit
TTATAAAGCAATGAGACTGATT71680.12498844798454385No Hit
AGCAGCATTGTACAGGGCTATG70820.1234888656008007No Hit
ATCCCGGACGAGCCCCCA69190.1206466338734736No Hit
ACTGGACTTGGAGTCAGAAGGCA68450.11935629554327602No Hit
TTCACAGTGGCTAAGTTCCG68250.11900755545403344No Hit
AACCGGGCGGAAACACCA67170.11712435897212348No Hit
AGAGGTAGTAGGTTGCATAGTT66170.1153806585259105No Hit
AGCAGCATTGTACAGGGCTATGT65630.11443906028495554No Hit
ACCACAGGGTAGAACCACGGAC64880.11313128495029581No Hit
CACTAGATTGTGAGCTCCTGGA64730.11286972988336387No Hit
TATTGCACTTGTCCCGGCCTGTAG64420.11232918274503786No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT62860.10960901004894567No Hit
TGTCAGTTTGTCAAATACCCCA62380.10877203383476346No Hit
TGAGAACTGAATTCCATGGGTT62010.10812686466966467No Hit
TTCACAGTGGCTAAGTTCCGC60490.10547643999142099No Hit
TAGCAGCACGTAAATATTGGCGT60290.1051276999021784No Hit
TCCCACTGCTTCACTTGACTAG60290.1051276999021784No Hit
TCGTACGACTCTTAGCGG59860.10437790871030683No Hit
TTCACAGTGGCTAAGTTCTGC59810.10429072368799619No Hit
CTCCCACTGCTTCACTTGACTAGCCT59590.10390710958982934No Hit
ACTGGACTTGGAGTCAGAAGG58170.10143105495620697No Hit
GACCCGCGGGCGCTCTCCAGTCCT57710.10062895275094902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)