FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6461154
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6365109.851336154501194No Hit
CAACGGAATCCCAAAAGCAGCTG4795397.4218785065330435No Hit
CCCCCACTGCTAAATTTGACTG2681884.150775542573355No Hit
AACATTCAACGCTGTCGGTGAGT1770142.739665391043148No Hit
AACATTCAACGCTGTCGGTGAG1393802.157199781958455No Hit
TTCAAGTAATCCAGGATAGGCT1271661.9681623437546913No Hit
TAGCAGCACGTAAATATTGGCG1114221.72449070243489No Hit
TAGCTTATCAGACTGATGTTGAC1001591.5501719971385917No Hit
TATTGCACTTGTCCCGGCCTGTAA989531.5315066008332257No Hit
AAGCTGCCAGTTGAAGAACTGT947841.4669825235553897No Hit
TAGCTTATCAGACTGATGTTGACT897481.389039790724691No Hit
TATTGCACTTGTCCCGGCCTGTAT766951.1870170560862658No Hit
GCCTGTCTGAGCGTCGCT725101.1222453450266006No Hit
TAGCTTATCAGACTGATGTTGA657111.0170164648606115No Hit
TATTGCACTTGTCCCGGCCTGT576250.8918685423687471No Hit
TGAGGTAGTAGGTTGTATAGTT556870.8618739005447015No Hit
TATTGCACTTGTCCCGGCCTGTA471450.7296684152707086No Hit
TCCTGTCTGAGCGTCGCT444790.6884064363734405No Hit
GGAATACCGGGTGCTGTAGGCTTT342890.5306946715710537No Hit
TGTAAACATCCTTGACTGGAAGCT330000.5107446750224496No Hit
TTAATGCTAATCGTGATAGGGGTT307220.4754878153345362No Hit
TGAGGTAGTAGATTGTATAGTT304210.4708292048138769No Hit
AGCAGCATTGTACAGGGCTATGA301920.4672849463114484No Hit
CATAAAGTAGAAAGCACTACT297170.45993331841339796No Hit
CACCCGGCTGTGTGCACATGTGC292580.4528293242971766No Hit
TGTAAACATCCCCGACTGGAAGCT283110.43817249983516876No Hit
ACCGGGTGCTGTAGGCTT246890.38211440247361383No Hit
TTCAAGTAATTCAGGATAGGTT246730.38186676869178476No Hit
CATTGCACTTGTCTCGGTCTGA238260.3687576553662086No Hit
CAACGGAATCCCAAAAGCAGCT231310.35800106296800854No Hit
TGAATACCGGGTGCTGTAGGCTTT229800.3556640191519967No Hit
CAACGGAATCCCAAAAGCAGCTGT227800.35256859687913333No Hit
GGAATACCGGGTGCTGTAGGCTT223820.34640870655613537No Hit
TCGTACGACTCTTAGCGG216820.33557472860111365No Hit
ACTGGACTTGGAGTCAGAAGGC203130.3143865631433642No Hit
ACCGGGTGCTGTAGGCTTT197050.30497647943385964No Hit
ACCCCCCACTGCTAAATTTGACTG194490.30101433892459456No Hit
TGATGTAGTAGGTTGTATAGTT194150.30048811713820783No Hit
ACCGGGTGCTGTAGGCTTA183130.28343234041473087No Hit
CCCCCACTGCTAAATTTGACTGGCTT182730.2828132559601582No Hit
CGCGACCTCAGATCAGACGTGG179180.2773188814258258No Hit
CAACACCAGTCGATGGGCTGT174290.26975057396867497No Hit
TGAATACCGGGTGCTGTAGGCTT146950.22743615149863322No Hit
CGCGACCTCAGATCAGACGTGGCG144800.22410857255530514No Hit
TAGCTTATCAGACTGATGTTGACA144240.2232418543189034No Hit
CCCATAAAGTAGAAAGCACTAC142210.2201000007119471No Hit
CCCATAAAGTAGAAAGCACT128510.19889635814283332No Hit
AACATTCAACGCTGTCGGTGAGTT126730.19614143231998496No Hit
CAAAGAATTCTCCTTTTGGGCT121600.1882016741900905No Hit
CACTAGATTGTGAGCTCCTGGA120540.18656110038547294No Hit
TTCTCACTACTGCACTTGACTAG117400.1817012874170775No Hit
TATTGCACTTGTCCCGGCCTGTAG116410.18016905339201017No Hit
TGATGTAGTAGATTGTATAGTT109470.1694279381051744No Hit
CACTAGATTGTGAGCTCCTGGAA109140.16891719343015196No Hit
TGAGGTAGTAGTTTGTACAGTT103700.1604976448479637No Hit
CTCCCCACTGCTAAATTTGACTG102930.1593059072729113No Hit
TCGTTTCCCGGCCAATGCACCA101940.15777367324784397No Hit
TATTGCACTTGTCCCGGCCTGTT101880.15768081057965805No Hit
CTCCCACTGCTTCACTTGACTAG98410.1523102529362402No Hit
TAGCAGCACGTAAATATTGGC97220.1504684766838865No Hit
CGCCCCACTGCTAAATTTGACTG94610.14642895061779984No Hit
CCCCCCACTGCTAAATTTGACTGG91550.14169295454031897No Hit
CTTCTCACTACTGCACTTGACTAG89580.1386439636015486No Hit
CCCGCCACTGCTAAATTTGACTG87500.1354247244377707No Hit
CCCCCCACTGCTAAATTTGACTGT86290.1335519939626884No Hit
TGAGAACTGAATTCCATGGGTT84310.1304875259125537No Hit
ACCTGGTGCTGTAGGCTT84170.13027084635345326No Hit
CGCGACCTCAGATCAGACGTGGC83070.12856836410337844No Hit
TATTGCACTTGTCCCGGCCTGTAAT82930.128351684544278No Hit
TACCACCCTGAACGCGCCCGA82240.12728376386014015No Hit
CAAAGAATTCTCCTTTTGGGCTT82000.12691231318739657No Hit
TGTAAACATCCTACACTCTCAGCT81760.12654086251465296No Hit
ACTTGACTTGGAGTCAGAAGGC80650.1248229031532138No Hit
AACGGAATCCCAAAAGCAGCTG79300.12273349311903106No Hit
TTATAAAGCAATGAGACTGATT77090.11931305150751709No Hit
TGAGGTAGTAGTTTGTGCTGTT75340.11660455701876166No Hit
TATTGCACTTGTCCCGGCCTGTAAA75260.11648074012784713No Hit
CCCCCCACTGCTAAATTTGACTGTA75080.11620215212328944No Hit
CGCCTGTCTGAGCGTCGCT71590.11080064025714292No Hit
CTCCCACTGCTTCACTTGACTAGCCT70240.10871123022296017No Hit
TGAGAACTGAATTCCATAGGCTGT67690.10476456682505943No Hit
ACCTGGTGCTGTAGGCTTT66460.10286088212724848No Hit
CCCCCCACTGCTAAATTTGACTGGCT66410.1027834965704269No Hit
AGCTCGGTCTGAGGCCCCTCAGT65310.10108101432035206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)