FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6812550
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG82370512.090993827568237No Hit
CAACGGAATCCCAAAAGCAGCTG4782437.020029210794783No Hit
CCCCCACTGCTAAATTTGACTG2673193.923919824441655No Hit
AACATTCAACGCTGTCGGTGAGT1554532.2818621514704476No Hit
TTCAAGTAATCCAGGATAGGCT1452052.1314338977328604No Hit
TAGCAGCACGTAAATATTGGCG1416222.0788397883318286No Hit
AACATTCAACGCTGTCGGTGAG1323031.9420481317568312No Hit
TATTGCACTTGTCCCGGCCTGTAT1277711.8755238493662432No Hit
TATTGCACTTGTCCCGGCCTGTAA1275141.871751399989725No Hit
AAGCTGCCAGTTGAAGAACTGT1045911.5352694659121768No Hit
TAGCTTATCAGACTGATGTTGACT923121.355028586946151No Hit
GCCTGTCTGAGCGTCGCT887511.3027574109547821No Hit
TAGCTTATCAGACTGATGTTGAC861011.263858613881733No Hit
TATTGCACTTGTCCCGGCCTGT644140.9455196659107089No Hit
TATTGCACTTGTCCCGGCCTGTA591770.8686468356195551No Hit
TGAGGTAGTAGGTTGTATAGTT560690.8230251521089754No Hit
TAGCTTATCAGACTGATGTTGA543880.7983501038524488No Hit
TGTAAACATCCTTGACTGGAAGCT433370.6361347806621602No Hit
AGCAGCATTGTACAGGGCTATGA368800.5413538249260555No Hit
TGTAAACATCCCCGACTGGAAGCT340100.49922569375637604No Hit
TGAGGTAGTAGATTGTATAGTT339500.49834496627547686No Hit
TTCAAGTAATTCAGGATAGGTT329270.48332856272614516No Hit
TCCTGTCTGAGCGTCGCT327740.4810827076498521No Hit
CCCCCACTGCTAAATTTGACTGGCTT312590.45884433875714675No Hit
GGAATACCGGGTGCTGTAGGCTTT283070.415512546696905No Hit
CACCCGGCTGTGTGCACATGTGC272700.40029064006869675No Hit
CTCCCACTGCTTCACTTGACTAG253360.3719018576010451No Hit
CAACGGAATCCCAAAAGCAGCTGT245010.3596450668251976No Hit
ACCCCCCACTGCTAAATTTGACTG243390.3572671026267697No Hit
CATAAAGTAGAAAGCACTACT235450.34561214229620335No Hit
ACTGGACTTGGAGTCAGAAGGC211870.3109995522968639No Hit
ACCGGGTGCTGTAGGCTTA196570.2885410015339337No Hit
CCCATAAAGTAGAAAGCACTAC193250.28366764280629136No Hit
TTCTCACTACTGCACTTGACTAG192540.2826254486205606No Hit
CATTGCACTTGTCTCGGTCTGA181000.26568612340459885No Hit
CAAAGAATTCTCCTTTTGGGCT178900.2626035772214516No Hit
TATTGCACTTGTCCCGGCCTGTAG174160.2556458301223477No Hit
GGAATACCGGGTGCTGTAGGCTT170480.2502440349061658No Hit
CACTAGATTGTGAGCTCCTGGAA169870.2493486286339183No Hit
CCCCCCACTGCTAAATTTGACTGGCT154170.22630292621705528No Hit
TGATGTAGTAGGTTGTATAGTT151800.22282405266750338No Hit
CACTAGATTGTGAGCTCCTGGA151530.2224277253010987No Hit
TAGCTTATCAGACTGATGTTGACA148840.21847913042840053No Hit
TATTGCACTTGTCCCGGCCTGTT144840.21260761388907237No Hit
CTTCTCACTACTGCACTTGACTAG144740.21246082597558918No Hit
ACCGGGTGCTGTAGGCTT143860.21116909233693698No Hit
AGCTCGGTCTGAGGCCCCTCAGT132040.1938187609632223No Hit
ACCGGGTGCTGTAGGCTTT130890.19213069995816545No Hit
CTCCCACTGCTTCACTTGACTAGCCT129200.1896499842202993No Hit
TATTGCACTTGTCCCGGCCTGTTT121050.1776867692714182No Hit
TGAATACCGGGTGCTGTAGGCTTT120390.17671796904242904No Hit
AACATTCAACGCTGTCGGTGAGTT120340.17664457508568746No Hit
CCCCCCACTGCTAAATTTGACTGG119030.1747216534190575No Hit
TGAGGTAGTAGTTTGTACAGTT117420.1723583680119779No Hit
CAACGGAATCCCAAAAGCAGCT112860.1656648391571438No Hit
TCGTACGACTCTTAGCGG112270.1647987904675929No Hit
CCCATAAAGTAGAAAGCACT111520.16369788111646888No Hit
TGTAAACATCCTACACTCTCAGCT109460.16067405009871485No Hit
TATTGCACTTGTCCCGGCCTGTAAA107610.1579584736992756No Hit
TATTGCACTTGTCCCGGCCTGTAAT102300.15016403549331747No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC101510.14900441097680017No Hit
CAAAGAATTCTCCTTTTGGGCTT98200.14414573104050613No Hit
TGATGTAGTAGATTGTATAGTT93540.1373054142721888No Hit
CCCCCCACTGCTAAATTTGACTGT89050.13071463695679297No Hit
CGCGACCTCAGATCAGACGTGGCG87880.1289972183690395No Hit
TAGCAGCACGTAAATATTGGC87550.1285128182545449No Hit
CAACACCAGTCGATGGGCTGT86880.12752933923420745No Hit
TGTAAACATCCCCGACTGGAAGC85990.12622292680420694No Hit
CCCCCCACTGCTAAATTTGACTGA84350.1238156050230824No Hit
CCCGCCACTGCTAAATTTGACTG81460.1195734343234178No Hit
AACGGAATCCCAAAAGCAGCTG81170.1191477493743165No Hit
GACCCGCGGGCGCTCTCCAGTCCT79670.11694593067206845No Hit
ATCCCGGACGAGCCCCCA76770.11268908118105556No Hit
TTCAAGTAATCCAGGATAGGCTT75370.1106340503922907No Hit
TCGTTTCCCGGGCGGCGCACCA73570.10799186794959302No Hit
TGAGGTAGTAGTTTGTGCTGTT72930.10705242530330053No Hit
GGGAATACCGGGTGCTGTAGGCTT71800.10539372188094033No Hit
AACCGGGCGGAAACACCA71710.10526161275880544No Hit
TTCAAGTAATCCAGGATAGGC70620.10366162450183851No Hit
TGAATACCGGGTGCTGTAGGCTT70060.10283961218633259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)