FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9002268
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG107032211.889470520095601No Hit
CAACGGAATCCCAAAAGCAGCTG6656467.394203327428155No Hit
GCCTGTCTGAGCGTCGCT3001923.3346263408287777No Hit
CCCCCACTGCTAAATTTGACTG2901063.2225879078472226No Hit
AACATTCAACGCTGTCGGTGAGT2340922.600366929755924No Hit
TATTGCACTTGTCCCGGCCTGTAT2075402.3054190344033305No Hit
TTCAAGTAATCCAGGATAGGCT2063652.2923667680189035No Hit
TAGCAGCACGTAAATATTGGCG1853792.059247736237135No Hit
TATTGCACTTGTCCCGGCCTGTAA1841242.0453068049073853No Hit
AACATTCAACGCTGTCGGTGAG1795871.9949083941957737No Hit
AAGCTGCCAGTTGAAGAACTGT1647251.8298166639784552No Hit
TAGCTTATCAGACTGATGTTGACT1234621.3714543934928398No Hit
TATTGCACTTGTCCCGGCCTGT1125001.2496850793600012No Hit
TAGCTTATCAGACTGATGTTGAC1122461.2468635681586018No Hit
TGAGGTAGTAGGTTGTATAGTT1058071.1753371483719437No Hit
TAGCTTATCAGACTGATGTTGA1014391.126816042357326No Hit
TCCTGTCTGAGCGTCGCT982221.091080603243538No Hit
TATTGCACTTGTCCCGGCCTGTA869450.9658121708884917No Hit
TGAGGTAGTAGATTGTATAGTT608900.6763851065087154No Hit
AGCAGCATTGTACAGGGCTATGA548400.6091798200186886No Hit
TGTAAACATCCTTGACTGGAAGCT481470.534831889030631No Hit
CTCCCACTGCTTCACTTGACTAG455760.5062724193503237No Hit
TTCAAGTAATTCAGGATAGGTT453850.5041507317933658No Hit
TGTAAACATCCCCGACTGGAAGCT435350.4836003549327792No Hit
ACTGGACTTGGAGTCAGAAGGC432110.4800012619042224No Hit
TGATGTAGTAGGTTGTATAGTT395060.4388449666239663No Hit
CATAAAGTAGAAAGCACTACT378510.42046071056760365No Hit
CAACGGAATCCCAAAAGCAGCTGT370530.41159627773801005No Hit
GGAATACCGGGTGCTGTAGGCTTT348250.3868469590107737No Hit
CATTGCACTTGTCTCGGTCTGA300620.3339380698286254No Hit
CACCCGGCTGTGTGCACATGTGC285050.31664242833028294No Hit
CCCCCACTGCTAAATTTGACTGGCTT262440.29152653531310113No Hit
TATTGCACTTGTCCCGGCCTGTAG259240.2879718755318104No Hit
ACCCCCCACTGCTAAATTTGACTG259050.28776081760729627No Hit
CACTAGATTGTGAGCTCCTGGA257200.2857057799212376No Hit
TTCTCACTACTGCACTTGACTAG247660.2751084504482648No Hit
TCGTACGACTCTTAGCGG242780.2696875942817965No Hit
TATTGCACTTGTCCCGGCCTGTT241880.26868784621830855No Hit
AGCTCGGTCTGAGGCCCCTCAGT241060.2677769646493528No Hit
CCCATAAAGTAGAAAGCACTAC237020.26328920667547334No Hit
CACTAGATTGTGAGCTCCTGGAA236480.26268935783738057No Hit
GGAATACCGGGTGCTGTAGGCTT235750.2618784510747736No Hit
ATCCCGGACGAGCCCCCA229550.2549912977485229No Hit
TGATGTAGTAGATTGTATAGTT220270.24468278438277996No Hit
CCCCCCACTGCTAAATTTGACTGG219400.2437163612547416No Hit
CCCCCCACTGCTAAATTTGACTGGCT213210.23684031624030744No Hit
CTTCTCACTACTGCACTTGACTAG212050.2355517520695896No Hit
TGAGGTAGTAGTTTGTACAGTT206670.22957548031229463No Hit
TATTGCACTTGTCCCGGCCTGTTT199890.22204404490068502No Hit
CCCATAAAGTAGAAAGCACT197270.2191336672047533No Hit
AACCGGGCGGAAACACCA184870.20535936055225196No Hit
GACCCGCGGGCGCTCTCCAGTCCT184280.20470397015507646No Hit
ACTTGACTTGGAGTCAGAAGGC179550.1994497386658562No Hit
CCCCCCACTGCTAAATTTGACTGT175850.19533966329373886No Hit
CAACACCAGTCGATGGGCTGT174650.19400666587575485No Hit
CGCCTGTCTGAGCGTCGCT171110.19007432349270206No Hit
CAACGGAATCCCAAAAGCAGCT168870.18758606164579858No Hit
TAGCTTATCAGACTGATGTTGACA167880.1864863387759618No Hit
CAAAGAATTCTCCTTTTGGGCT157510.1749670194222167No Hit
CTCCCACTGCTTCACTTGACTAGCCT156680.17404502954144443No Hit
TATTGCACTTGTCCCGGCCTGTAAT152630.16954616325574845No Hit
AACATTCAACGCTGTCGGTGAGTT150500.16718009283882684No Hit
TGAATACCGGGTGCTGTAGGCTTT137000.1521838718865068No Hit
TGAGGTAGTAGTTTGTGCTGTT130520.14498568582939322No Hit
TGTAAACATCCTACACTCTCAGCT129720.14409702088407056No Hit
ACTGGACTTGGAGTCAGAAGGCA128550.14279734840153616No Hit
TATTGCACTTGTCCCGGCCTGA123900.13763198340684812No Hit
TGTAAACATCCCCGACTGGAAGC119920.13321087530386788No Hit
CCCCCCACTGCTAAATTTGACTGTA118550.1316890365850028No Hit
TAGCAGCACGTAAATATTGGC118200.13130024567142412No Hit
AACGGAATCCCAAAAGCAGCTG117880.13094477969329507No Hit
TTCAAGTAATCCAGGATAGGCTT117470.1304893389088172No Hit
TATTGCACTTGTCCCGGCCTGTAAA116510.12942294097443No Hit
CGCCCCACTGCTAAATTTGACTG115520.12832321810459318No Hit
AGCAGCATTGTACAGGGCTATGT109840.12201369699280226No Hit
CAAAGAATTCTCCTTTTGGGCTT108030.12000309255400972No Hit
ACCGGGTGCTGTAGGCTTA107050.11891447799598945No Hit
TGAGGTAGTAGGTTGTGTGGTT107040.11890336968417292No Hit
ACTGGACTTGGAGTCAGAAGGCT104020.11554865951557985No Hit
CTCCCCACTGCTAAATTTGACTG99840.11090538517626891No Hit
TGAGGTAGTAGGTTGTATAGT98220.10910583866199051No Hit
CCCCCCACTGCTAAATTTGACTGA97720.10855042307116386No Hit
TTATAAAGCAATGAGACTGATT97040.10779505786763957No Hit
CCCTGTCTGAGCGTCGCT96470.10716188409409717No Hit
CTACGCCTGTCTGAGCGTCGCT94180.10461808068811104No Hit
TGAATACCGGGTGCTGTAGGCTT92510.10276299261474998No Hit
TTCAAGTAATCCAGGATAGGC91070.10116339571316917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)